Maldi biotyper sirius system

Manufactured by Bruker

Sourced in Germany, United States

The MALDI Biotyper Sirius system is a mass spectrometry-based microbial identification platform designed for rapid, reliable, and cost-effective microbial identification. The system utilizes matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) technology to generate unique protein profiles, which are then compared against a comprehensive reference database for accurate identification of microorganisms.

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Lab products found in correlation

Flexanalysis v 3, by Bruker (2 mentions) Flexcontrol 3, by Bruker (2 mentions) 4800 proteomics analyzer, by Thermo Fisher Scientific (1 mentions) Data explorer version 4, by Thermo Fisher Scientific (1 mentions) Zirconium beads, by Biospec (1 mentions) Magna lyser, by Roche (1 mentions) 2 hydroxy 5 methoxybenzoic acid super dhb, by Merck Group (1 mentions) 2 5 dihydroxybenzoic acid, by Merck Group (1 mentions) Asl buffer, by Qiagen (1 mentions)

Close spelling variants of this product

Biotyper Sirius ROU MALDI-ToF system MALDI Biotyper Sirius Biotyper Sirius system MALDI Biotyper system MALDI Biotyper Biotyper system

9 protocols using maldi biotyper sirius system

MALDI-TOF Mass Spectrometry Protocol

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MS analyses were performed on a MALDI Biotyper Sirius system (Bruker Daltonik, Germany). The mass spectra were scanned in the range of m/z 400 to 1,000. The mass profiles were acquired using FlexControl 3.4 software (Bruker Daltonik, Germany). The spectra were recorded in the linear positive-ion mode (laser intensity 95%, ion source 1 = 10.00 kV, ion source 2 = 8.98 kV, lens = 3.00 kV, detector voltage = 2652 V, pulsed ion extraction = 150 ns). Each spectrum corresponded to ion accumulation of 5,000 laser shots randomly distributed on the spot. The spectra obtained were processed with default parameters using FlexAnalysis v.3.4 software (Bruker Daltonik, Germany).
Assignments were based on the MS/MS fragmentation profile acquired on a 4800 Proteomics Analyzer (with TOF-TOF Optics, Applied Biosystems, plate: 384 Opti-TOF 123 mm × 84 mm AB Sciex NC0318050, 1016629) using the reflectron mode. Samples were analyzed operating at 20 kV in the positive ion mode. MS/MS mass spectrometry data were analyzed using Data Explorer version 4.9 from Applied Biosystems.

England P., Tang W., Kostrzewa M., Shahrezaei V, & Larrouy-Maumus G. (2020). Discrimination of bovine milk from non-dairy milk by lipids fingerprinting using routine matrix-assisted laser desorption ionization mass spectrometry. Scientific Reports, 10, 5160.

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Quantifying S. aureus in Mouse Tissues

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Samples were collected in 2 ml tubes containing 0.5 ml saline with 5 sterilized zirconium beads (Ø 2 mm, BioSpec Products) and stored on ice. To neutralize mouse endogenous antimicrobial peptide (AMP) activity, 50 μl of a 0.05% (v/v) sodium polyanethole sulfonate (SPS; Sigma) solution was added to the samples [35 (link)]. Due to technical issues, no SPS was added to samples from the P4HB, PP and sham non-infected mice at 9 days and samples from the sham infected mice at 9 days. Samples were homogenized with 3 cycles of 30 s at 7000 rpm with 30 s cooling on ice in between the cycles (MagNA Lyser, Roche). Of this homogenate, 100 μl was ten-fold serially diluted in phosphate buffered saline (PBS) and five-fold 10 μl aliquots of the sample and the dilutions were spotted on blood agar plates. After overnight incubation, colonies were counted and converted to log CFU per homogenized biopsy. Morphologically distinct colonies were selected for species determination (MALDI Biotyper, Sirius System, Bruker) to rule out contamination. In case of bacterial contamination other than S. aureus, these colonies were not included in the CFU counts.

Verhorstert K.W., Riool M., Bulten T., Guler Z., de Boer L., Roovers J.P, & Zaat S.A. (2022). The impact of bacterial contamination on the host response towards fully absorbable poly-4-hydroxybutyrate and nonabsorbable polypropylene pelvic floor implants. Materials Today Bio, 15, 100268.

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MALDI-TOF MS Bacterial Identification Protocol

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A 10-μl inoculation loop of bacteria, grown on Mueller-Hinton agar for 18 to 24 h, was resuspended in 200 μl of water. Mild acid hydrolysis was performed on 100 μl of this suspension, by adding 100 μl of 2% (vol/vol) acetic acid and incubating the mixture at 98°C for 5 min. Hydrolyzed cells were centrifuged at 17,000 × g for 2 min, the supernatant was discarded, and the pellet was resuspended in ultrapure water to a McFarland standard of 10. A 0.4-μl aliquot of this suspension was loaded onto the target and immediately overlaid with 1.2 μl of a matrix consisting of a 9:1 mixture of 2,5-dihydroxybenzoic acid and 2-hydroxy-5-methoxybenzoic acid (super-DHB) (Sigma-Aldrich) dissolved in 90:10 (vol/vol) chloroform/methanol to a final concentration of 10 mg/ml. The bacterial suspension and matrix were mixed directly on the target by pipetting, and the mixture was dried gently under a stream of air for less than 1 min. MALDI-TOF MS analysis was performed with a MALDI Biotyper Sirius system (Bruker Daltonics), using the newly introduced linear negative-ion mode.

Furniss R.C., Dortet L., Bolland W., Drews O., Sparbier K., Bonnin R.A., Filloux A., Kostrzewa M., Mavridou D.A, & Larrouy-Maumus G. (2019). Detection of Colistin Resistance in Escherichia coli by Use of the MALDI Biotyper Sirius Mass Spectrometry System. Journal of Clinical Microbiology, 57(12), e01427-19.

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MALDI-TOF Mass Spectrometry Protocol

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MS analyses were performed on an MALDI Biotyper Sirius system (Bruker Daltonics, Germany). The mass spectra were scanned in the range of m/z 1,100–1,600. The mass profiles were acquired using FlexControl 3.4 software (Bruker Daltonics, Germany). The spectra were recorded in the linear positive ion mode (laser intensity 95%, ion source 1 = 10.00 kV, ion source 2 = 8.98 kV, lens = 3.00 kV, detector voltage = 2652 V, pulsed ion extraction = 150 ns). Each spectrum corresponded to an ion accumulation of 5,000 laser shots randomly distributed on the spot. The spectra obtained were processed with default parameters using FlexAnalysis v.3.4 software (Bruker Daltonics, Germany).

Jia Khor M., Broda A., Kostrzewa M., Drobniewski F, & Larrouy-Maumus G. (2021). An Improved Method for Rapid Detection of Mycobacterium abscessus Complex Based on Species-Specific Lipid Fingerprint by Routine MALDI-TOF. Frontiers in Chemistry, 9, 715890.

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MALDI-TOF Identification of Candida species

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The isolates were identified using MALDI-TOF with the Microflex LT mass spectrometer and a MALDI Biotyper Sirius system (Bruker). The MALDI Biotyper CA system has been FDA approved for the identification of all Candida species that have been included in this study and is CE-IVD certified (approved by the European Commission for in vitro use) as well. MBT Compass software version 4.1.100 and the Bruker Taxonomy database version 9 were used. Identification of yeasts was first conducted automatically in research use only (RUO) mode. The default “spiral_small” pattern of the MALDI Biotyper was used for the automatic acquisition and identification of the spot-plated colonies. With the acceptance criterion for identification on the species level set at a score of ≥1.8 (validated in-house), yeasts with an identification score of <1.8 were reanalyzed on the MALDI-TOF through manual reshooting of the target. The same workup was performed for the manually spot-plated strains.

Heestermans R., Herroelen P., Emmerechts K., Vandoorslaer K., De Geyter D., Demuyser T., Wybo I., Piérard D, & Muyldermans A. (2022). Validation of the Colibrí Instrument for Automated Preparation of MALDI-TOF MS Targets for Yeast Identification. Journal of Clinical Microbiology, 60(7), e00237-22.

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MALDI-TOF Identification of Microbial Isolates

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Five macroscopically identical microbial colonies isolated from each sample/culture medium, as part of the analyses mentioned in Chapter 2.2, were sub-cultured on Mueller–Hinton agar (37 °C, 24–48 h) with subsequent identification using matrix-assisted laser desorption/ionization–time-of-flight (MALDI-TOF) mass spectrometry (MS). Each strain for identification was transferred to the target steel plate in the form of a thin film followed by overlaying 1 μL of the matrix (α-cyano-4-hydroxycinnamic acid). After drying, the plates were prepared for analysis in a MALDI Biotyper Sirius System (Bruker Daltonics GmbH, Bremen, Germany) in linear positive ion mode over the m/z range of 2000 to 20,000. Based on the TOF information, a characteristic spectrum was generated using MBT Compass Software (MBT Compass Library Revision H 2021). An achieved identification score of 2.00–3.00 represents a high-confidence identification (species level), 1.70–1.99 represents a low-confidence identification (genus level) and a score of 0–1.69 represents an unsuccessful strain identification.

Šilha D., Syrová P., Syrová L, & Janečková J. (2022). Smoothie Drinks: Possible Source of Resistant and Biofilm-Forming Microorganisms. Foods, 11(24), 4039.

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Epidemiology of Candidemia in Catania

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The study involved 629 yeast isolates from positive blood cultures of hospitalized patients at the University Hospital in Catania. The period in which the selected patients were hospitalized fluctuated between January 2017 and December 2021. All the isolates were taken from a mycoteca, inoculated in Sabouraud Dextrose agar plates, and added with 2% of glucose (Vakutest Kima, Arzergrande, Italy). Plates were incubated for at least 24–72 h at a temperature of 37 °C. Species or genus identification were confirmed through MALDI Biotyper^® Sirius System (Bruker, Billerica, MA, USA) for all the selected isolates. The following microorganisms were identified: C. albicans (283), C. glabrata (53), C. krusei (23), C. tropicalis (68), C. parapsilosis complex (151), S. cerevisiae (12), C. neoformans (5), S. capitata (12), Rhodotorula species (4), C. famata (6), and C. guilliermondii (12).

Calvo M., Scalia G., Palermo C.I., Oliveri S, & Trovato L. (2023). Comparison between EUCAST Broth Microdilution and MIC Strip Test in Defining Isavuconazole In Vitro Susceptibility against Candida and Rare Yeast Clinical Isolates. Antibiotics, 12(2), 251.

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Multiplex PCR for Aeromonas Identification

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Stool samples were transported from the community clinics and were tested daily except on weekends, where part of each sample was suspended in ASL buffer (Qiagen, Hilden, Germany) and refrigerated at 4 °C until tested by PCR only (culturing was done from the original sample tube). Multiplex PCR for Aeromonas and other bacterial pathogens was performed as previously described [7 (link)]. Stool samples that were positive by PCR for Aeromonas were inoculated into alkaline peptone water 0.5 M NaCl with Cephalothin (10 mg/l) and incubated overnight [8 (link)] followed by sub-culturing onto SS agar plates (Hylabs, Rehovot, Israel). Presumptive Aeromonas colonies were identified by MALDI Biotyper Sirius system (Bruker Daltonics, Bremen, Germany) using the MBT IVD Library Revision software. Definite determination of species was based on whole genome sequencing as described below.

Sagas D., Hershko Y., Levitskyi K., Strauss M., Slutzkin M., Chazan B, & Adler A. (2024). Phenotypic and genotypic analysis of antimicrobial resistance and population structure of gastroenteritis-related Aeromonas isolates. Annals of Clinical Microbiology and Antimicrobials, 23, 45.

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GBS Detection from Swab Specimens

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Based on SEIMC, 2015 [4 ] procedures, swab specimens were cultured with automation systems WASP^® (Walk Away Specimen Processor) (Copan, Brescia, Italy) on the specific, selective and differential medium Granada (Oxoid^®, Hampshire, UK) at 35 °C in anaerobic conditions by covering the inoculated plate with a cover slide. Simultaneously, swabs were cultured on the selective medium for Gram-positive Columbia CNA (Colistin and Nalidixic Acid) agar (bioMerieux^®, Marcy-l’Étoile, France) at 35 °C in aerobic conditions, to look for β-haemolytic colonies. If no GBS presence was obtained after 48 h incubation, the sample was considered negative. The isolates’ identification was confirmed by matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry (MALDI Biotyper^® sirius System, Bruker Daltonics, Billerica, MA, USA) using the MBT Compass Library Rev L 2020 and the software version MBT Compass, and rapid agglutination as an alternative technique.

Peris M.P., Martín-Saco G., Alonso-Ezcurra H., Escolar-Miñana C., Rezusta A., Acero R, & Milagro-Beamonte A. (2022). Retrospective Study for the Clinical Evaluation of a Real-Time PCR Assay with Lyophilized and Ready-to-Use Reagents for Streptococcus agalactiae Detection in Prenatal Screening Specimens. Diagnostics, 12(9), 2189.

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