Streptavidin biotin complex sabc kit

Manufactured by Boster Bio

Sourced in United States

The Streptavidin-Biotin Complex (SABC) kit is a laboratory tool designed to facilitate high-affinity interactions between streptavidin and biotin. This complex is widely used in various immunoassay techniques, such as ELISA, Western blotting, and immunohistochemistry, to detect and quantify target molecules.

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Lab products found in correlation

Imager a1 microscope, by Zeiss (1 mentions) Rabbit anti f4 80 antibody, by Santa Cruz Biotechnology (1 mentions) Imager a2 fluorescence microscope, by Zeiss (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Roche (1 mentions) Nonessential amino acid, by Thermo Fisher Scientific (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Hoechst 33342, by Merck Group (1 mentions) Image pro plus 6, by Media Cybernetics (1 mentions) Vascular endothelial growth factor (vegf), by Immunoway (1 mentions) Bcl 2, by Immunoway (1 mentions) Hif 1α, by Immunoway (1 mentions) Tnf α, by Immunoway (1 mentions) Caspase3, by Immunoway (1 mentions) Il 1β, by Immunoway (1 mentions) Fitc conjugated goat anti mouse igg, by Proteintech (1 mentions) Tritc conjugated goat anti rat igg, by Proteintech (1 mentions)

Close spelling variants of this product

Streptavidin-Biotin Complex solution (SABC kit, Streptavidin-biotin-peroxidase complex (SABC) kit Streptavidin Biotin Complex kit SABC kit

6 protocols using streptavidin biotin complex sabc kit

Immunolabeling Techniques for Tissue Analysis

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Immunohistochemistry (IHC) and immunofluorescence analyses were performed according to standard protocols. IHC was performed using a ready-to-use StreptAvidin Biotin-Complex (SABC) kit (Boster Biological Technology Co. Ltd., Wuhan, China). Sections were incubated with a rabbit anti-F4/80 antibody (Santa Cruz Biotechnology Inc., Dallas, TX, USA; 1:500 dilution), a rabbit anti-CK-19 antibody (Bioss; 1:200 dilution), a rabbit anti-amylase antibody (Bioss; 1:200 dilution), a rabbit anti-CD3 antibody (Bioss; 1:200 dilution), and a goat anti-COX-2 antibody (Santa Cruz; 1:200 dilution) for 48 h at 4°C. The corresponding secondary antibodies were subsequently incubated for 1 h at room temperature. Images were captured using a ZEISS Imager A1 microscope (Carl Zeiss AG, Oberkochen, Germany).
For double staining of immunofluorescence, a 1:200 dilution of COX-2 antibody and a 1:400 dilution of F4/80 antibody or CK-19 antibody was incubated in a humid chamber for 48 h at 4°C, and a 1:500 dilution of FITC-labeled anti-goat or CY3-labeled anti-rabbit secondary antibodies was incubated for 1.5 h. Nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) (Roche) for 5 min, dehydrated, and mounted on a coverslip with anti-fade mounting medium. Images were captured with a ZEISS Imager A2 fluorescence microscope (Carl Zeiss AG).

Xu X.F., Fan J.W., Xin J.Q., Wu N., Gao H., Duan L.F., Zou W.B., Zhang H, & Li Z.S. (2022). Aspirin Ameliorates Pancreatic Inflammation and Fibrosis by Inhibiting COX-2 Expression in Experimental Chronic Pancreatitis. Journal of Inflammation Research, 15, 4737-4749.

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Optimized Transfection of Sox9 Plasmid in Cells

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All experimental protocols were approved by the First Affiliated Hospital of Nanjing Medical University and followed the principles of laboratory and animal care of the university. (2,3-Dioleoyloxy-propyl)-trimethylammonium (DOTAP) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) were purchased from A.V.T. (Shanghai) Pharmaceutical Co., Ltd. Hoechst 33342 and 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (Cambridge, MA, USA). Sox9 plasmid was purchased from Bioworld Technology, Inc., China (PPL00081-2b) a, and the species of the Sox9 plasmid is Homo sapiens (human) with vector backbone of pcDNA3 (Figure S1). Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were bought from Gibco BRL (Thermo Fisher, USA). Additionally, Penicillin-streptomycin, 0.25% trypsin-EDTA, and non-essential amino acid were obtained from Invitrogen (Thermo Fisher), anti-Collagen II (ab34712) and anti-collagen IX (ab134568) were bought from Abcam (USA), Streptavidin-Biotin Complex (SABC) kit was purchased from Boster (SA1025, USA). Other chemicals used in this work were all of the analytical pure grades and were used as received.

Zhang G., Nie M., Webster T.J., Zhang Q, & Fan W. (2019). Ectopic chondrogenesis of nude mouse induced by nano gene delivery enhanced tissue engineering technology. International Journal of Nanomedicine, 14, 4755-4765.

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Immunohistochemistry for CD31 and CD34

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Immunohistochemistry (IHC) procedure was performed using the streptavidin biotin complex (SABC) kit (Boster Biological Technology). Briefly, after deparaffinization and rehydration, the tissue sections were microwaved for 10 min in citrate buffer for antigen retrieval and then incubated separately with primary rabbit polyclonal antibody against CD31 (1:100; cat. no. ab28364; Abcam) and primary rabbit polyclonal antibody against CD34 (1:100; cat. no. ab110643; Abcam) overnight at 4 °C to stain target protein expression. Following 20-min incubation with secondary anti-rabbit antibody, 3,3-diaminobenzidine (DAB) was applied for visualizing immunoreactivity. Five randomly selected fields in each slice were quantified and the integrated optical density (IOD) immunoreactivity was calculated as the relative products of the stained area using Image Pro Plus 6.0 (Media Cybernetics, Silver Spring, MD, USA).

Xiang R., Zhang Q.P., Zhang W., Kong Y.G., Tan L., Chen S.M., Deng Y.Q., Tao Z.Z, & Xu Y. (2018). Different effects of allergic rhinitis on nasal mucosa remodeling in chronic rhinosinusitis with and without nasal polyps. European Archives of Oto-Rhino-Laryngology, 276(1), 115-130.

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Immunohistochemical and Immunofluorescent Staining

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Immunohistochemistry and immunofluorescence were performed with the Streptavidin-Biotin Complex (SABC) kit (Boster Bio, Pleasanton, CA, USA). Following dewaxing, paraffin sections were placed in heated 3% citric acid and subsequently incubated with 3% H₂O₂ for 10 min to remove endogenous oxidases. The sections were then washed three times with PBS and blocked with 5% goat serum for 30 min at 37°C. After rewashing, the sections were incubated with a rabbit IgG primary antibody (IL-1β, IL-6, TNF-α, VEGF; 1:200; ImmunoWay) overnight at 4°C. Subsequently, the samples were rewashed, treated with biotinylated goat anti-rabbit IgG drop-wise (1:200), and incubated for 30 minutes at 37°C. SABC-POD or SABC-FITC reagent was then added dropwise to the samples, and the preparation was incubated for 30 minutes at 37°C. The primary antibody was replaced with PBS for the negative controls. The sections intended for immunohistochemistry were stained with DAB for 70 seconds and counterstained with hematoxylin, mounted with neutral gum, and photographed under the microscope, whereas sections intended for immunofluorescence were photographed after mounting with anti-quenching mounting tablets. Immunohistochemical-positive cells were described by integral optical density using Image-pro plus 6.0.

Wang Y., Wang X., Wang Y.X., Ma Y, & Di Y. (2022). The Long-Noncoding RNA TUG1 Regulates Oxygen-Induced Retinal Neovascularization in Mice via MiR-299. Investigative Ophthalmology & Visual Science, 63(1), 37.

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Immunohistochemical Analysis of Cellular Markers

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Immunohistochemistry was performed with the Streptavidin-Biotin Complex (SABC) kit (Boster Bio, Pleasanton, CA, United States). The paraffin sections were first dewaxed and thermally repaired with a 3% citric acid repair solution. Then, 3% H₂O₂ was added dropwise and incubated at room temperature for 10 min. The sections were washed three times with PBS and blocked with goat serum at 37°C for 30 min. Then, the sections were incubated with diluted primary antibody [CCN1, hypoxia-inducible factor-1 α (HIF-1α), VEGF, caspase-3, Bcl-2, IL-1β, and TNF-α, 1:200, ImmunoWay, Plano, TX, United States] at 4°C overnight. On the second day, the sections were washed with PBS three times. Biotinylated goat anti-rabbit IgG was dropped and incubated at 37°C for 30 min, then washed with PBS three times. The SBC-POD reagent was added dropwise to the samples and incubated at 37°C for 30 min. For negative control groups, the primary antibody was replaced with PBS. Under the microscope, 3, 3′-diaminobenzidine was applied for 50 s and counterstained with hematoxylin, sealed with neutral gum, and then observed and photographed under the microscope. Brown cells were positive for the respective antibody, and ImageJ was used to calculate the cumulative optical density of each immunohistochemical section by the double-blind method.

Wang Y., Wang Y., Wang X., Ma Y., Li Z, & Di Y. (2022). LncRNA TUG1 Promotes Apoptosis, Invasion, and Angiogenesis of Retinal Endothelial Cells in Retinopathy of Prematurity via MiR-145-5p. Frontiers in Medicine, 9, 803214.

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Immunofluorescence and Histochemical Analysis of Muscle Vasculature

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Mice were perfused with PBS containing 4% paraformaldehyde through a cannula inserted into the left ventricle. The calf muscle was excised, embedded in O.C.T. compound (Sakura Fine Chemical, Tokyo, Japan), frozen on dry ice, and sectioned (8 μm). For immunofluorescence measurements, acetone-fixed frozen sections were incubated with rat monoclonal anti-CD31/PECAM-1 antibody (1:100, clone MEC13.3, BD Biosciences, San Jose, CA) and mouse monoclonal anti-αsmooth muscle actin (αSMA) (1:100, clone 1A4, Sigma). Bound antibodies were detected using TRITC-conjugated goat anti-rat IgG (Proteintech, Chicago, USA) and FITC-conjugated goat anti-mouse IgG (Proteintech) secondary antibodies. For immunohistochemistry, tissue sections probed with primary antibodies were incubated with StreptAvidin-Biotin Complex (SABC) kit, (Boster, Wuhan, P.R. China), followed by visualization with 3,3’–diaminobenzidine tetrahydrochloride (Maxim, Fuzhou, P.R. China).
CD31-positive capillary densities were counted in ten randomly chosen high power fields per mouse and expressed as the number of capillaries per mm². Quantification of the extent of αSMA-positive microvasculatures was carried out using an Image J (NIH) system.

Qi X., Yuan Y., Xu K., Zhong H., Zhang Z., Zhai H., Guan G, & Yu G. (2015). (2-Hydroxypropyl)-β-Cyclodextrin Is a New Angiogenic Molecule for Therapeutic Angiogenesis. PLoS ONE, 10(5), e0125323.

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