Anti erα

All the chemicals and drugs including 4-hydroxy-tamoxifen (Tam or 4OH-Tam;
Cat#H7904) and 17β-estradiol (E2; Cat#E2257) were purchased from
Sigma-Aldrich (St Louis, MO, USA). CCN5 human recombinant protein (hrCCN5;
Cat#120–16) was purchased from PeproTech (Rocky Hill, NJ, USA).
Doxycycline (Dox) was purchased from Takara Bio (Mountain View, CA, USA, Cat #
631311). Antibodies for western blot analysis, immunofluorescence,
immunohistochemical staining were purchased from following vendors: Anti-ERα
(Cell Signaling, Danvers, MA, USA; Cat#13258 and Abcam, Cambridge, MA, USA;
Cat#ab32063), anti-p-ER-α (Cell Signaling, Cat#9924), Anti-CCN5
(Abcam, Cat#ab38317), anti-Akt (Cell Signaling, Cat#4691), anti-p-Akt
(Cell Signaling, Cat # 9271), mouse Anti-FLAG/DDK (Origene, Rockville, MD,
USA, Cat#TAG0011), mouse anti-β-actin (Sigma, Cat#A3853),
anti-Integrins α6 (Millipore, Billerica, MA, USA, Cat#MAB1378) and
anti-Integrins β1 (Millipore, Cat#MAB1987Z). The authentication
certificates for all these chemicals, drugs and antibodies were provided by these
companies. The fresh working solutions of the chemicals and drugs were prepared
once a month to guarantee effectivity.

Tamoxifen was purchased from Nacalai Tesque (Tokyo, Japan). Caffeine, caffeic acid, chlorogenic acid, pyrocatechol, and trigonelline were purchased from Sigma-Aldrich (St. Louis, MO). LY294002 and U0126 were purchased from Tocris Bioscience (Bristol, UK). Anti-ERα, anti-phospho-MEK1/2 (S217/221), anti-MEK1/2, anti-phospho-ERK1/2 (T202/Y204), anti-ERK1/2, anti-phospho-Akt (S473), anti-Akt, and anti-cleaved caspase-3 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-p53, anti-cyclin D1, and anti-β-actin antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Peroxidase-conjugated rabbit anti-mouse, goat anti-rabbit, and swine anti-goat secondary antibodies were from Dako (Glostrup, Denmark).