Human umbilical vein ECs (HUVECs) and 293 cells were purchased from the Shanghai Institutes for Biological Sciences (CAS). HUVECs were cultured in endothelial cell medium (ECM) supplemented with 5% fetal bovine serum (FBS), 1% endothelial cell growth supplement (ECGS), penicillin (100 U/ml) and streptomycin (100 mg/ml) (Sciencell, Inc.). When HUVEC confluency reached approximately 70-80%, FBS in ECM was replaced by 5% exosome-free FBS (System Biosciences) and HUVECs were stimulated with or without 100 µM H2O2 for 24 h to produce exosomes. HUVECs incubated with exosomes were all cultured in basal medium (without FBS). 293 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% FBS, penicillin (100 U/ml) and streptomycin (100 µg/ml). All the cells were cultured in a 5% CO2 incubator at 37°C (Thermo Fisher Scientific, Inc.).
Huvecs
Manufactured by System Biosciences
Sourced in United States
HUVECs are primary human umbilical vein endothelial cells. They are a type of cell line derived from the endothelium of human umbilical veins.
Automatically generated - may contain errors
Lab products found in correlation
Co2 incubator, by Thermo Fisher Scientific (1 mentions)
Exosome free fbs, by System Biosciences (1 mentions)
Fetal bovine serum (fbs), by System Biosciences (1 mentions)
Endothelial cell growth supplement (ecgs), by ScienCell (1 mentions)
Huvecs, by ScienCell (1 mentions)
Penicillin streptomycin, by ScienCell (1 mentions)
2 protocols using huvecs
1
Exosome Production from Stressed HUVECs
2
Senescent HUVEC-derived Exosome Isolation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human umbilical vein endothelial cells (HUVECs) were purchased from ScienCell Research Laboratories (Carlsbad, CA). Cells were maintained at 37 °C in a humidified atmosphere of 5 % CO2 in the air and cultured in endothelial cell medium (ECM) supplemented with 5 % heat-inactivated FBS, 1 % penicillin-streptomycin and 1 % endothelial cell growth supplement (ScienCell Research Laboratories). Senescent HUVECs at passage 22-30 were cultured in conditioned culture medium consisted of ECM supplemented with 5 % exosome-depleted FBS (System Biosciences, United States) and the conditioned medium was recovered for exosomes isolation. Senescence in HUVECs was confirmed by flow cytometry using fluorogenic substrate C12FDG to measure senescence-associated-beta-galactosidase (SA-β-gal) activity. Experiments that involved treatment with senescent HUVECs-secreted exosomes were performed using young HUVECs at passage 9-11 cultured in the conditioned culture medium.
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