Mammary glands, uterus, vagina, kidneys, and liver were dehydrated by a series of ethanol solution, embedded in paraffin blocks before cutting into 5 μm sections, and stained with hematoxylin and eosin as previously reported by Zingue et al. [9 (link), 15 (link)]. Histomorphological changes were determined under an Axioskop 40 microscope connected to a computer where the image was transferred using MRGrab1.0 and Axio Vision 3.1 software (Zeiss Hallbergmoos, Germany). Atlas and histologic classification of tumors of rat mammary gland from Russo and Russo [16 ] were used in this study.
Mrgrab1
Manufactured by Zeiss
Sourced in Germany
The MRGrab1.0 is a laboratory equipment product designed for precise and controlled manipulation of small objects. It features a robotic arm with multiple degrees of freedom, allowing for delicate and intricate handling tasks. The core function of the MRGrab1.0 is to provide researchers and engineers with a versatile tool for handling and positioning small samples or components with high accuracy and repeatability.
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Lab products found in correlation
16 protocols using mrgrab1
1
Histomorphological Analysis of Rat Organs
2
Histopathological Examination of Organs
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Organs (mammary gland, lung, kidney, liver, and brain) embedded in paraffin were cut into 5 μm sections and stained with hematoxylin and eosin. Histoarchitectures of abovementioned organs were examined using Axioskop 40 microscope connected to a computer where the images were transferred using MRGrab1.0 and Axio Vision 3.1 softwares, all from Zeiss (Hallbergmoos, Germany).
3
Histological Analysis of Reproductive Tissues
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In addition, 5 μm thick sections of paraffin embedded tissues (uterus, vagina and ovaries) were prepared and stained with hematoxylin–eosin. The photomicroscopic observation/analysis (uterine and vaginal epithelial heights, identification of the ovarian follicles) was performed using a complete set of Zeiss (Hallbermoos, Germany) equipment (microscope Axioskop 40 and the software programs MRGrab 1.0 (Carl Zeiss, Hallbermoos, Germany, 2001) and AxioVision 3.1 (Carl Zeiss, Hallbermoos, Germany, 2001) installed in a computer). Regarding the folliculogenesis, the tenth section from each ovary was selected. We considered as primary the follicles composed of oocytes surrounded with one layer of cuboidal follicular cells; secondary or preantral follicles those with more than one follicular cell layer; and antral follicles those with present antrum of follicular fluid and graafian follicles with one single large antrum of follicular fluid. Ruptured follicles with hypertrophied follicular cells and cavity filled with blood were considered as corpora lutea.
4
Histomorphological Analyses of Organ Changes
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Histomorphological changes in mammary glands, ovaries, uterus, brain and liver were determined by using 5 μm tissue sections of paraffin-embedded organs stained with hematoxylin and eosin. Histological sections were observed and images were captured using the complete Zeiss equipment consisting of a microscope Axioskop 40 connected to a computer where the images were transferred and analyzed with the MRGrab 1.0 and Axio Vision 3.1 softwares, all provided by Zeiss (Hallbermoos, Germany). The mammary tumors were classified using the well characterized criteria [40 (link)].
5
Tissue Preparation and Histological Analysis
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Liver, lung, and kidneys were dehydrated by a series of ethanol solution and embedded in paraffin blocks before cutting into 5 μm sections. These sections were stained with hematoxylin and eosin (HE) and examined under an Axioskop 40 microscope connected to a computer where the image was transferred using MRGrab1.0 and Axio Vision 3.1 softwares (Zeiss, Hallbermoos, Germany).
6
Histomorphology of Mammary and Reproductive Tissues
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Using the complete Zeiss equipment consisting of a microscope Axioskop 40 connected to a computer where the image was transferred, and analyzed with the MRGrab1.0 and Axio Vision 3.1 softwares, all provided by Zeiss (Hallbermoos, Germany), the histomorphology of the mammary glands, as well as the uterine and vaginal epithelial heights, were assessed from 5-μm sections of paraffin-embedded tissues following hematoxylin-eosin staining.
7
Histological Assessment of Organ Changes
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Vaginal smears were taken using an eyedropper containing 10 mL of NaCl 0.9%, placed on ringed slides, fixed in ethanol 95% for 30 min, and stained with a Papanicolaou method [31 ]. Cellular types were observed under a light microscope using 400 magnifications. To determine the histomorphological changes in mammary gland, uterus, vagina, femur, VL-3, mandibular bone, liver, lungs, and kidneys, paraffin-embedded organs were cut to 5 μm tissue sections and stained with hematoxylin and eosin. Stained sections were visualized and images captured using the complete Zeiss equipment consisting of a microscope Axioskop 40 connected to a computer where the images were transferred and analyzed with the MRGrab1.0 and Axio Vision 3.1 software, all provided by Zeiss (Hallbergmoos, Germany).
8
Histological Analysis of Mammary and Reproductive Tissues
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The formalin-fixed tissues were embedded in paraffin, and sections of 5-μm thickness were cut. Following hematoxylin-eosin staining, mammary gland differentiation, uterine and vaginal epithelial height were assessed on microphotography using the complete Zeiss equipment consisting of a microscope Axioskop 40 connected to a computer where the image was transferred, and analyzed with the MRGrab 1.0 and Axio Vision 3.1 softwares, all provided by Zeiss (Hallbermoos, Germany).
9
Histomorphological Analysis of Organ Tissues
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Organs (mammary glands, uterus, vagina, femur, brain, liver, and lung) were dehydrated by a series of ethanol solution and embedded in paraffin blocks before cutting into 5 μm sections and stained with hematoxylin and eosin. Histomorphological changes were determined under an Axioskop 40 microscope connected to a computer where the image was transferred using MRGrab1.0 and Axio Vision 3.1 software (Zeiss, Hallbergmoos, Germany). Atlas and histologic classification of tumors of rat mammary gland from J. Russo and I. H. Russo [24 ] were used in this study.
10
Histomorphological Brain Analysis
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The histomorphology of the brain was performed from 5 μm sections of paraffin-embedded tissues following hematoxylin-eosin staining. Brains were photographed at 40x magnification using the complete Zeiss equipment consisting of a microscope Axioskop 40 connected to a computer where the image was transferred, and analyzed with the MRGrab1.0 and AxioVision 3.1 software, all provided by Zeiss (Hallbergmoos, Germany).
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