Hepaticult organoid growth medium

Manufactured by STEMCELL

HepatiCult Organoid Growth Medium is a specialized cell culture medium designed to support the growth and maintenance of human hepatic organoids. It provides the necessary nutrients and growth factors to facilitate the expansion and differentiation of liver progenitor cells into mature hepatocyte-like cells within a three-dimensional organoid structure.

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Lab products found in correlation

Prestoblue hs cell viability reagent, by Thermo Fisher Scientific (1 mentions) Matrigel, by Corning (1 mentions) Intesticult organoid growth medium, by STEMCELL (1 mentions)

2 protocols using hepaticult organoid growth medium

Mouse Hepatic Progenitor Organoid Assay

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Mouse hepatic progenitor organoids (70932, STEMCELL Technologies) were seeded in 96-well plate using 20 ul Matrigel (Corning, #354230) and cultured in HepatiCult Organoid Growth Medium (06031, STEMCELL Technologies) with or without TM2. Medium was replaced after every 48 hr with fresh compound. Organoid viability was measured using PrestoBlue HS Cell Viability Reagent (Thermo Fisher, # P50200) following the manufacturer’s protocol.

Hu L., Sun Y., Liu S., Erb H., Singh A., Mao J., Luo X, & Wu X. (2022). Discovery of a new class of reversible TEA domain transcription factor inhibitors with a novel binding mode. eLife, 11, e80210.

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Scalable Organoid Culture Protocol

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For organoid cultures, we used cryopreserved mouse intestinal organoids (70931, STEMCELL Technologies) and cryopreserved mouse hepatic progenitor organoids (70932, STEMCELL Technologies) derived from the small intestine and the liver of C57BL/6 mice, respectively. Intestinal and liver organoids were cultured in 24-well plates according to the manufacturer’s protocols using IntestiCult organoid growth medium (06005, STEMCELL Technologies) and Hepaticult organoid growth medium (06030, STEMCELL Technologies), respectively. To briefly explain, we injected 50 μl of the organoid/Matrigel mixture (1:1, v/v) into 24-well plates to form Matrigel drops, which were then incubated at 37°C and 5% CO₂ for 10 min to allow gelation of Matrigel. Upon completion of this step, 750 μl of pre-warmed organoid growth medium was added to each well. Organoids were passaged every 5–7 d in fresh Matrigel until use as recommended by the manufacturer.

Sutton A.J., Duval S.J., Tweedie R.L., Abrams K.R, & Jones D.R. (2000). Empirical assessment of effect of publication bias on meta-analyses. BMJ : British Medical Journal, 320(7249), 1574-1577.

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