Protosappanin B was purchased from Shanghai Yuanye Bio-Technology Co. Ltd. (B21622-20 mg, Shanghai, China). Reagents, signaling pathway agonists and antibodies were purchased from the following companies: RPMI-1640 medium, DMEM medium (Shanghai Yuanye Bio-technology, Shanghai, China); Streptavidin Peroxidase immunohistochemistry kit (GS4961, GS4962), cell counting kit-8 (QN1293), Annexin V-FITC/PI double staining apoptosis detection kit (SNM530), and ECL western blotting detection kit (QN1155) (Beijing BioRab Technology Co. Ltd., Beijing, China); BCA protein assay kit (C05-02001), western blocking buffer (5% BSA, C05-06002) (Beijing Biosynthesis Biotechnology Co., Ltd, Beijing, China); IGF-1 (K002504P), AZD2858 (A126819), and TPA (bs-1545R) (Shanghai Hengfei Biotechnology Co. Ltd. Shanghai, China); Anti-GOLPH3 (ab98023), Anti-p-AKT (ab8805), Anti-AKT (ab38449), and anti-PCNA (proliferating cell nuclear antigen) (ab18197) (Abcam, Cambridge, UK); Anti-p-p70S6K (ribosomal protein S6 kinase, 70 kDa) (sc-8416), Anti-p70S6K (sc-8418), Anti-β-catenin (sc-7963), Anti-p-ERK1/2(extracellular signal-regulated kinase 1 and 2) (sc-81492), Anti-ERK1/2 (sc-514302), Anti-GAPDH (sc-365062), and secondary antibodies (sc-516102, sc-2357) (Santa Cruz Biotechnology, Inc., Dallas, TX, USA).
Ab98023
Manufactured by Abcam
Sourced in China, United States
Ab98023 is a lab equipment product manufactured by Abcam. It is a device designed for specific laboratory applications. The core function of this product is to facilitate certain experimental procedures, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Ab18197, by Abcam (1 mentions)
Ab8805, by Abcam (1 mentions)
Anti β catenin sc 7963, by Santa Cruz Biotechnology (1 mentions)
Bca protein assay kit, by Bioss Antibodies (1 mentions)
Sc 516102 sc 2357, by Santa Cruz Biotechnology (1 mentions)
Sc 8418, by Santa Cruz Biotechnology (1 mentions)
Rpmi 1640 medium, by Yuanye Bio-Technology (1 mentions)
Ab38449, by Abcam (1 mentions)
Ab109268, by Abcam (1 mentions)
Ab126171, by Abcam (1 mentions)
Ab208943, by Abcam (1 mentions)
Ab192890, by Abcam (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
2 protocols using ab98023
1
Protosappanin B Anti-Cancer Protocol
2
Western Blot Analysis of Neurological Markers
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N2a cells, the ipsilateral cortex of SD rats, and OM-MSCs were processed for western blotting as described previously. Proteins were transferred to the PVDF membrane (Millipore, USA) when the bromophenol blue reached the bottom, and the blots were blocked in 4% BSA in TBST (0.05%) solution for 1 h at room temperature and then incubated at 4°C overnight with the corresponding primary antibody. After incubation with secondary antibodies at room temperature for at least 1 h, the blot was visualized by the ChemiDoc XRS imaging system.
The primary antibodies were as follows: anti-caspase3 (19677-1-AP, Proteintech, USA), anti-GOLPH3 (ab98023, Abcam, Cambridge, UK), anti-SPCA1 (ab126171, Abcam, Cambridge, UK), anti-LC3 (ab192890, Abcam, Cambridge, UK), anti-LAMP1 (ab208943, Abcam, Cambridge, UK), anti-Akt (60203-2-Ig, Proteintech, Chicago, USA), anti-p-Akt (ab38449, Abcam, Cambridge, UK), anti-mTOR (20657-1-AP, Proteintech, Chicago, USA), anti-p-mTOR (ab109268, Abcam, Cambridge, UK), anti-PEDF (bs-20784R, Bioss, Beijing, China), and anti-β-actin (66009-1-Ig, Proteintech, Chicago, USA). The anti-rabbit and anti-mouse IgG secondary antibodies were obtained from Proteintech (Chicago, USA).
The primary antibodies were as follows: anti-caspase3 (19677-1-AP, Proteintech, USA), anti-GOLPH3 (ab98023, Abcam, Cambridge, UK), anti-SPCA1 (ab126171, Abcam, Cambridge, UK), anti-LC3 (ab192890, Abcam, Cambridge, UK), anti-LAMP1 (ab208943, Abcam, Cambridge, UK), anti-Akt (60203-2-Ig, Proteintech, Chicago, USA), anti-p-Akt (ab38449, Abcam, Cambridge, UK), anti-mTOR (20657-1-AP, Proteintech, Chicago, USA), anti-p-mTOR (ab109268, Abcam, Cambridge, UK), anti-PEDF (bs-20784R, Bioss, Beijing, China), and anti-β-actin (66009-1-Ig, Proteintech, Chicago, USA). The anti-rabbit and anti-mouse IgG secondary antibodies were obtained from Proteintech (Chicago, USA).
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