Goat polyclonal anti doublecortin

Brain removal, processing, sectioning, and immunohistochemical detection of BrdU and other cell markers were performed as previously described^{28 (link),29 (link)}. Primary antibodies used were: mouse monoclonal anti-BrdU (1:100) and rabbit polyclonal anti-GFAP (1:3000) from Dako (Glostrup, Denmark); rat monoclonal anti-BrdU (1:100) from Abcam (Cambridge, UK); goat polyclonal anti-doublecortin (1:200) and goat polyclonal anti-nestin (1:200) from Santa Cruz Biotechnology (Santa Cruz, CA, USA); mouse monoclonal anti-NeuN (1:100), goat polyclonal anti-ChAT (1:100), and mouse monoclonal anti-parvalbumin (1:100) from Merk Millipore (Billerica, MA, USA), and mouse monoclonal anti-VGLUT2 (1:100) from Abcam (Cambridge, UK). Secondary fluorescent antibodies used (1:1000) were all from Invitrogen (Carlsbad, CA, USA): donkey anti-rabbit IgG conjugated to AlexaFluor 594 or AlexaFluor 488; donkey anti-mouse IgG conjugated to AlexaFluor 405 or AlexaFluor 488; donkey anti-goat conjugated to AlexaFluor 594; and donkey anti-rat conjugated to AlexaFluor 488 or AlexaFluor 594. Biotinylated donkey anti-goat IgG (1:250) was from Sigma-Aldrich.

Brain removal, processing, sectioning and immunohistochemical detection of BrdU and other cell markers were performed as previously described (Moreno-López et al., 2004 (link); Rabaneda et al., 2008 (link)). Primary antibodies used were: mouse monoclonal anti-BrdU (1:100) from Dako (Glostrup, Denmark), goat polyclonal anti-doublecortin (1:200) from Santa Cruz Biotechnology (Santa Cruz, CA, USA) Secondary fluorescent antibodies used (1:1,000) were all from Invitrogen (Carlsbad, CA, USA): donkey anti-goat IgG conjugated to AlexaFluor 594 and donkey anti-mouse IgG conjugated to AlexaFluor 488.