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Cd34 pe cy7

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The CD34-PE-CY7 is a fluorescently-labeled antibody that can be used to detect and analyze CD34-positive cells in flow cytometry applications. It is composed of a CD34-specific antibody conjugated to the PE-Cy7 fluorescent dye.

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Lab products found in correlation

Facsaria 2, by BD (4 mentions) Cd38 pe, by Thermo Fisher Scientific (2 mentions) Cd38 apc, by Thermo Fisher Scientific (2 mentions) Cd45 fitc, by Thermo Fisher Scientific (2 mentions) Facscan flow cytometer, by Beckman Coulter (2 mentions) Cd184 brilliant violet 421, by BioLegend (2 mentions) Cd144 pe, by Thermo Fisher Scientific (2 mentions) Cd19 pe cy7, by BD (1 mentions) Lsrii flow cytometer, by BD (1 mentions) Cd45 apc, by BD (1 mentions) Cd123 apc, by Thermo Fisher Scientific (1 mentions) Cd90 percpcy5, by Thermo Fisher Scientific (1 mentions) Cd47 apc, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 conjugated goat anti rabbit antibody, by Thermo Fisher Scientific (1 mentions) Cd133 pe, by Miltenyi Biotec (1 mentions) Influx cell sorter, by BD (1 mentions) Cd10 apc, by BioLegend (1 mentions) Cd19 pe, by BioLegend (1 mentions) Cd14 apc cy7, by BioLegend (1 mentions)

Spelling variants of this product

CD34-PE (38 citations) CD34-PE Cy7 clone 4H11 (2 citations)

10 protocols using cd34 pe cy7

1

Assessing Apoptosis in CML Stem Cells

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Bone marrow samples were obtained with written informed consent from three patients with CML. This study was approved by the Ethical Review Committee for Biomedical Research of Fujian Medical University. Mononuclear cells from CML bone marrow were treated with or without XN4 for 48 h. The cells were labeled with CD34-PE-Cy7 and CD38-PE (eBioscience Inc, CA, USA) and then analyzed by flow cytometry for apoptosis via Annexin V-APC staining.
Wu L., Chen X., Huang L., Tian J., Ke F., Xu J., Chen Y, & Zheng M. (2015). A Novobiocin Derivative, XN4, Inhibits the Proliferation of Chronic Myeloid Leukemia Cells by Inducing Oxidative DNA Damage. PLoS ONE, 10(4), e0123314.
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2

Phenotypic Characterization of MSCs

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MSCs were also characterized by flow cytometry after three cell culture passages. MSCs were stained with the following commercially available antibodies: CD105-PE (PE mouse anti-human CD105, Cat. 323205, BioLegends); CD34-PE-Cy7 (mouse PE-Cyanine7 anti-human CD34, Cat. 25-0349-42, eBioscience); CD45-APC (mouse APC anti-human CD45, Cat. 555485, BD Pharmingen™); CD19-PE-Cy  7 (mouse PE-Cy™7 anti-human CD19, Cat. 557835, BD Pharmingen); CD73-APC (mouse APC anti-human CD73, Cat. 560847, BD Pharmingen); CD146-PE (mouse PE anti-human CD146, Cat. 550315, BD Pharmingen); CD14-PE (mouse PE anti-human C14 antibody, Cat. 301805, BioLegend); CD90-APC (mouse APC anti-human CD90, Clone 5E10 (RUO), Cat. 559869, BD Biosciences). Antibody staining was performed at 4°C in PBS containing 5% (v/v) fetal calf serum (FCS). Approximately 50,000–100,000 cells were stained per well. Cells were acquired using a LSR II flow cytometer (Becton Dickinson). Analysis was performed using FlowJo (Tree Star).
Juneja S.C., Viswanathan S., Ganguly M, & Veillette C. (2016). A Simplified Method for the Aspiration of Bone Marrow from Patients Undergoing Hip and Knee Joint Replacement for Isolating Mesenchymal Stem Cells and In Vitro Chondrogenesis. Bone Marrow Research, 2016, 3152065.
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3

Flow Cytometric Immunophenotyping Assay

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The following antibodies were used for these studies: KDR (clone 89106), CD235a-APC (clone HIR2), CD4-PE-Cy7 (clone RPA-T4), CD5-PE-Cy7 (clone L17F12), CD7-FITC (clone M-T701), CD8-PE (clone RPA-T8), CD34-APC (clone 8G12), CD34-PE-CY7 (clone 4H11), CD43-PE or FITC (clone 1G10), CD45-APC-eFluor750 (clone 2D1), CD56-PE or APC (clone B159), CD117-APC (clone 104D2), CD144-PE (clone 123413). All antibodies were purchased from BD Biosciences (San Diego, CA) except CD34-PE-CY7 which was purchased from eBioscience (San Diego, CA), and KDR was purchased from R&D systems. Cells were sorted with FACSAria™II (BD) cell sorter at the Sick Kids/UHN Flow Cytometry Facility.
Sturgeon C.M., Ditadi A., Awong G., Kennedy M, & Keller G. (2014). Wnt Signaling Controls the Specification of Definitive and Primitive Hematopoiesis From Human Pluripotent Stem Cells. Nature biotechnology, 32(6), 554-561.
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4

Quantifying SIRT1 Expression in CD34+ Cells

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CD34+ cells were labeled with CD34-PeCy7, Lin-APC-Cy7 (including CD2, CD3, CD7, CD10, CD19, and CD235a), and CD38-APC or CD38-PE, CD90-PercpCy5.5, CD123-APC, or CD47-APC (eBioscience) antibodies; fixed and permeabilized (Cytofix/Cytoperm Kit, Beckman Coulter); labeled with rabbit anti-human SIRT1 (E1104-1, Millipore) and Alexa Fluor 488-conjugated goat anti-rabbit antibodies (Invitrogen); and analyzed by flow cytometry. Data were analyzed using FlowJo software (version 8.5.2; TreeStar).
Li L., Osdal T., Ho Y., Chun S., McDonald T., Agarwal P., Lin A., Chu S., Qi J., Li L., Hsieh Y.T., Santos C.D., Yuan H., Ha T.Q., Popa M., Hovland R., Bruserud Ø., Gjertsen B.T., Kuo Y.H., Chen W., Lain S., McCormack E, & Bhatia R. (2014). SIRT1 Activation by a c-MYC Oncogenic Network Promotes the Maintenance and Drug Resistance of Human FLT3-ITD Acute Myeloid Leukemia Stem Cells. Cell stem cell, 15(4), 431-446.
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5

Enumeration of Hematopoietic Stem Cells

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At day 10, we harvested the nonadherent and adherent cells from five different culture conditions, and the total nucleated cells (TNCs) counted via trypan blue staining method. Flow cytometry staining was performed with CD34-PECY7, CD45-FITC, and CD38-APC (eBioscience, San Diego, USA) antibodies, then samples were analyzed using a FACScan flow cytometer (Beckman Coulter, USA). For each sample, at least 10,000 events were recorded. The isotype antibodies were used to determine the level of nonspecific binding.
Li Q., Zhao D., Chen Q., Luo M., Huang J., Yang C., Wang F., Li W, & Liu T. (2019). Wharton’s jelly mesenchymal stem cell-based or umbilical vein endothelial cell-based serum-free coculture with cytokines supports the ex vivo expansion/maintenance of cord blood hematopoietic stem/progenitor cells. Stem Cell Research & Therapy, 10, 376.
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6

Flow Cytometric Analysis of Gene-Targeted Cells

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Gene‐targeted cells were analyzed by flow cytometry (LSR Fortessa; Becton Dickinson Pharmingen). Immunophenotypic analysis of the hematopoietic differentiated cells was performed using CD34 PE‐Cy7 (eBioscience), CD133 PE (Miltenyi Biotech) and CD90 APC (Becton Dickinson Pharmingen) antibodies according to the manufacturer's instructions:
Fluorochrome‐matched isotypes were used as controls. 4′,6‐diamidino‐2‐phenylindole (DAPI; Roche)‐positive cells were excluded from the analysis. Analysis was performed using FlowJo software v7.6.5.
Cell sorting was also used to select gene‐edited LCLs by the selection of EGFP+ cells in a BD Influx cell sorter (BD Biosciences).
Diez B., Genovese P., Roman‐Rodriguez F.J., Alvarez L., Schiroli G., Ugalde L., Rodriguez‐Perales S., Sevilla J., Diaz de Heredia C., Holmes M.C., Lombardo A., Naldini L., Bueren J.A, & Rio P. (2017). Therapeutic gene editing in CD34+ hematopoietic progenitors from Fanconi anemia patients. EMBO Molecular Medicine, 9(11), 1574-1588.
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7

Flow Cytometric Immunophenotyping Assay

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The following antibodies were used for these studies: KDR (clone 89106), CD235a-APC (clone HIR2), CD4-PE-Cy7 (clone RPA-T4), CD5-PE-Cy7 (clone L17F12), CD7-FITC (clone M-T701), CD8-PE (clone RPA-T8), CD34-APC (clone 8G12), CD34-PE-CY7 (clone 4H11), CD43-PE or FITC (clone 1G10), CD45-APC-eFluor750 (clone 2D1), CD56-PE or APC (clone B159), CD117-APC (clone 104D2), CD144-PE (clone 123413). All antibodies were purchased from BD Biosciences (San Diego, CA) except CD34-PE-CY7 which was purchased from eBioscience (San Diego, CA), and KDR was purchased from R&D systems. Cells were sorted with FACSAria™II (BD) cell sorter at the Sick Kids/UHN Flow Cytometry Facility.
Sturgeon C.M., Ditadi A., Awong G., Kennedy M, & Keller G. (2014). Wnt Signaling Controls the Specification of Definitive and Primitive Hematopoiesis From Human Pluripotent Stem Cells. Nature biotechnology, 32(6), 554-561.
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8

Multicolor Flow Cytometry Immunophenotyping

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The following antibodies were used for these studies: CD34-APC (clone 8G12, 1:100), CD34-PE-CY7 (clone 4H11, 1:100), CD43-PE or FITC (clone 1G10, 1:30 and 1:10 respectively), CD4-PE-Cy7 (clone RPA-T4, 1:100), CD8-PE (clone RPA-T8, 1:30), CD45-APC-Cy7 or eFluor450 (clone 2D1, 1:50), CD56-APC (clone B159, 1:30), CD144-PE (clone 123413, 1:50), CD184-Brilliant Violet 421 (clone 12G5, 2:100), CD73-APC (clone AD2, 1:400), KDR (clone 89106, 1:7) and CD235a-APC (clone HIR2, 1:50). Most antibodies were purchased from BD Biosciences (San Diego, CA). The exceptions are CD34-PE-CY7 and CD144-PE that were purchased from eBioscience, CD184-Brilliant Violet 421 purchased from Biolegend and KDR purchased from R&D systems. Cells were sorted with FACSAria™II (BD) cell sorter at the Sick Kids/UHN Flow Cytometry Facility.
Ditadi A., Sturgeon C.M., Tober J., Awong G., Kennedy M., Phillips A., Azzola L., Ng E.S., Stanley E., French D.L., Cheng X., Gadue P., Speck N., Elefanty A.G, & Keller G. (2015). HUMAN DEFINITIVE HAEMOGENIC ENDOTHELIUM AND ARTERIAL VASCULAR ENDOTHELIUM REPRESENT DISTINCT LINEAGES. Nature cell biology, 17(5), 580-591.
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9

Multicolor Flow Cytometry Immunophenotyping

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The following antibodies were used for these studies: CD34-APC (clone 8G12, 1:100), CD34-PE-CY7 (clone 4H11, 1:100), CD43-PE or FITC (clone 1G10, 1:30 and 1:10 respectively), CD4-PE-Cy7 (clone RPA-T4, 1:100), CD8-PE (clone RPA-T8, 1:30), CD45-APC-Cy7 or eFluor450 (clone 2D1, 1:50), CD56-APC (clone B159, 1:30), CD144-PE (clone 123413, 1:50), CD184-Brilliant Violet 421 (clone 12G5, 2:100), CD73-APC (clone AD2, 1:400), KDR (clone 89106, 1:7) and CD235a-APC (clone HIR2, 1:50). Most antibodies were purchased from BD Biosciences (San Diego, CA). The exceptions are CD34-PE-CY7 and CD144-PE that were purchased from eBioscience, CD184-Brilliant Violet 421 purchased from Biolegend and KDR purchased from R&D systems. Cells were sorted with FACSAria™II (BD) cell sorter at the Sick Kids/UHN Flow Cytometry Facility.
Ditadi A., Sturgeon C.M., Tober J., Awong G., Kennedy M., Phillips A., Azzola L., Ng E.S., Stanley E., French D.L., Cheng X., Gadue P., Speck N., Elefanty A.G, & Keller G. (2015). HUMAN DEFINITIVE HAEMOGENIC ENDOTHELIUM AND ARTERIAL VASCULAR ENDOTHELIUM REPRESENT DISTINCT LINEAGES. Nature cell biology, 17(5), 580-591.
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10

Isolation and Characterization of Cord Blood Hematopoietic Stem and Progenitor Cells

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We defined the CD34+, CD10−, CD14−, and CD19− cells as CD34+Linage− (CD34+Lin−) cells, which represent cord blood HSPCs. At day 7, we collected the supernatant and detached the adherent cells containing WJ-MSCs and HSPCs (attached to the WJ-MSCs). Viable total nucleated cells (TNC) were counted via the Trypan Blue staining method. Flow cytometry staining was performed with CD34-PECY7, CD45-FITC (eBioscience, San Diego, CA, USA), CD10-APC, CD14-APC/Cy7, and CD19-PE (Biolegend, San Diego, CA, USA) antibodies, and then samples were analyzed using a FACScan flow cytometer (Beckman Coulter, USA). For each sample, at least 30,000 events were recorded. The isotype antibodies were used to determine the level of nonspecific binding.
Zhao D., Liu L., Chen Q., Wang F., Li Q., Zeng Q., Huang J., Luo M., Li W., Zheng Y, & Liu T. (2018). Hypoxia with Wharton’s jelly mesenchymal stem cell coculture maintains stemness of umbilical cord blood-derived CD34+ cells. Stem Cell Research & Therapy, 9, 158.
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