A total of 3-μm-thick consecutive tissue sections were prepared from formalin-fixed and paraffin-embedded tissues. Deparaffinization, rehydration, antigen retrieval, and peroxidase blocking were performed on a fully automated system BENCHMARK ULTRA (Roche) according to manufacturer recommendations. The sections were incubated with the following primary antibodies: anti-CD68 clone Kp1 (Dako) and anti-DPD (Thermo Fisher Scientific). Revelation was performed using the Ultraview DAB revelation kit (Roche). Nuclei were counterstained with hematoxylin solution (Dako). Images were captured using an APERIO ATS scanner (Leica).
Ultraview dab revelation kit
Manufactured by Roche
Sourced in Switzerland
The Ultraview DAB revelation kit is a laboratory equipment product designed for the detection of specific target proteins in tissue samples. It utilizes a diaminobenzidine (DAB) chromogenic substrate to visualize the target proteins, providing a clear and reliable method for immunohistochemical analysis.
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2 protocols using ultraview dab revelation kit
1
Immunohistochemical Analysis of CD68 and DPD
2
Immunohistochemical Evaluation of HER2 Expression
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HER2 protein expression was assessed by immunohistochemistry. Tissue slides of 3 to 4 µm thickness were prepared from formalin-fixed paraffin-embedded (FFPE) GEC blocks, and the immunoreaction was performed with an anti-HER2/neu monoclonal antibody (clone 4B5), followed by ultraView DAB Revelation Kit (Roche, Switzerland), according to the manufacturer's recommendations. The criteria for the HER2 interpretation score system established by Hofmann et al. (11 (link)) was used: negative result, score 0, no reactivity or membrane reactivity in less than 10% of neoplastic cells; negative result, score 1+, weak or incomplete membrane reactivity in 10% or more neoplastic cells (reactive cells in only part of the membrane); doubtful or suspicious result, score 2+, complete to basolateral or lateral weak to moderate membrane reactivity in 10% or more of neoplastic cells; positive result, 3+ score, was complete strong basolateral or lateral membrane reactivity in 10% or more of neoplastic cells (11 (link),16 (link),17 (link)).
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