Elite 1

The GC-MS analysis was carried out using a Clarus 500 PerkinElmer (Auto system XL) Gas Chromatograph equipped and coupled to a mass detector Turbo mass gold - PerkinElmer Turbomass 5.1 spectrometer with an Elite - 1 (100% Dimethylpolysiloxane), 30 m × 0.25 mm ID × 1 μm capillary column. The instrument was set to an initial temperature of 70 °C and maintained at this temperature for 3 min. At the end of this period, the oven temperature was increased to 300 °C, increasing 10 °C/min, and maintained for 9 min. Injection port temperature was ensured at 250 °C and Helium flow rate at 1.5 ml/min. The ionization voltage was 70 eV. The samples were injected in split mode as 10:1. Mass spectral scan range was set at 40–700 (m/z). The ion source temperature was maintained at 230 °C, and the interface temperature was at 240 °C. The MS start time was 3 min, and the end time was 35 min, with the solvent cut time of 3 min. The identification of components was accomplished by comparing retention time, fragmentation pattern, and mass spectra on a NIST Ver. 11 MS data library and comparing the spectrum obtained through GC-MS, compounds present in the plant samples were identified. The relative percentage of each extract constituent was expressed as a percentage with peak area normalization.

The GC-MS analysis was carried out using a Clarus 500 Perkin-Elmer (Auto system XL) Gas Chromatograph equipped and coupled to a mass detector Turbo mass gold - Perkin Elmer Turbomass 5.1 spectrometer with an Elite - 1 (100% Dimethyl poly siloxane), 30 m × 0.25 mm ID × 1 μm of capillary column. The instrument was set to an initial temperature of 70°C, and maintained at this temperature for 3 min. At the end of this period the oven temperature was rose up to 300°C, at the rate of an increase of 10°C/min, and maintained for 9 min. Injection port temperature was ensured at 250°C and Helium flow rate at 1.5 ml/min. The ionization voltage was 70 eV. The samples were injected in split mode as 10:1. Mass spectral scan range was set at 40-700 (m/z). The ion source temperature was maintained at 230°C and Interface temperature was at 240°C. The MS start time was 3 min, and end time was 35 min with solvent cut time was of 3 min.
Using computer searches on a NIST Ver. 11 MS data library and comparing the spectrum obtained through GC-MS, compounds present in the plant samples were identified.