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5 protocols using glass fiber

1

BALB/c Mice Lateral Flow Assay

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Nitrocellulose membranes, absorbent pads, glass fibers and PVC plates were purchased from Millipore Corp (Billerica, MA, USA). An XYZ-3060 dispensing platform and CM4000 Guillotine Cutter (BioDot, Irvine, CA, USA) were used to prepare test strips. A membrane strip reading instrument (TSR5000) was purchased from Jiening Biotech Co., Ltd. (Shanghai, China). A Forma Series II CO2 incubator was supplied by Thermo Electron (Waltham, MA, USA). A vacuum drying oven was purchased from Shanghai Senxin Experimental Instrument Co., Ltd. (Shanghai, China). A dehumidifier was purchased from Zhuyan Biological Co., Ltd. (Nanjing, China).
BALB/c female mice were purchased from the Center of Comparative Medicine of Yangzhou University (Yangzhou, China).
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2

Development of Fluoroquinolone Antibody Assay

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MBF, N-hydroxy succinimide (NHS), N, N-dimethylformamide (DMF), Na2B4O7, 1-(3-(dimethylamino) propyl)-3-ethylcarbodiimide hydrochloride (EDC), Freund’s adjuvant, and a mouse monoclonal antibody isotyping kit were purchased from Sigma (St Louis, MO, USA). ENR, CIP, NOR, OFL, and DIF were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). HRP-conjugated goat-anti-mouse IgG was purchased from Sino-American Biotechnology Co., Ltd. (Luoyang, China). BSA and OVA were both obtained from Yuanye Biotechnology Co., Ltd. (Shanghai, China). Filter membranes, nitrocellulose (NC) membranes, absorbent pads, and glass fibers were purchased from Millipore (Bedford, MA, USA).
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3

Fluorescent Labeling of Proteins Using QDs

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Orange–red (λem = 610 nm, particle size = 24 nm) ZnCdSe/ZnS QDs were purchased from Wuhan Jiayuan Quantum Dots Corporation, Ltd. (Wuhan, China). 1-(3-dimethylaminopropyl)-3-ethylcar-bodiimide hydrochloride (EDC-HCl) and Bovine serum albumin (BSA) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). BCA Protein Assay Kits (PC0020) were purchased from Sorlarbio Life Sciences (Beijing, China). Staphylococcal protein A (SPA) was purchased from Beijing Bersee Science and Technology Corporation, Ltd. (Beijing, China). HRP-conjugated anti-His-Tag monoclonal antibody (5C3) and HRP-conjugated goat anti-mouse IgG were purchased from Abbkine Scientific Corporation, Ltd. (Wuhan, China). eECL Western Blot Kit was purchased from CWbiotech Corporation, Ltd. (Jiangsu, China). Nitrocellulose membranes, glass fiber and absorbent pads were purchased from Millipore (Bedford, MA, USA).
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Rapid Lateral Flow Immunoassay

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Pluronic F127, potassium tetrachloroplatinate(II) (K2PtCl4), sodium tetrachloropalladate(II) (Na2PdCl4), hydrochloric acid (HCl), ascorbic acid, potassium carbonate (K2CO3), acetone, sucrose, HRP, hydrogen peroxide (H2O2), 3,3′,5,5′-tetramethylbenzidine (TMB), Tween-20, sucrose, phosphate-buffered saline (1× PBS, pH 7.4, 0.01 M), and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (St. Louis, MO). Mouse anti-S. Enteritidis monoclonal antibody, mouse anti-E. coli O157:H7 monoclonal antibody, and goat antimouse IgG antibody were obtained from Abcam Inc. (Cambridge, MA). Goat anti-S. Enteritidis polyclonal antibody and goat anti-E. coli O157:H7 polyclonal antibody were purchased from KPL Inc. (Gaithersburg, MD). Nitrocellulose membrane, glass fiber, backing cards, and an absorbent pad were purchased from Millipore (Billerica, MA).
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5

Lateral Flow Assay for M. pneumoniae

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Chloroauric acid (HAuCl4·3H2O) and sodium citrate were purchased from the company of Sigma (USA). Bovine serum albumin (BSA) and goat anti-mouse IgG were purchased from DAKO (DK). High-flow nitrocellulose membrane, glass fiber, and absorption pad were purchased from Millipore (Bedford, MA). The mouse momoclonal antibodies against MP (named P-Ab1501 and P-Ab1502) were prepared by Benxi TestJet Biotechnologies. Bovine serum albumin (BSA) and goat anti-mouse IgG were purchased from Sigma (USA). Single-walled nanotubes were purchased from Beijing DK Daoking Technology Co.Standard M. pneumoniae FH (ATCC 15531) and M129 (ATCC 29342) strains were purchased from ATCC. Haemophilus influenzae, Streptococcus pneumoniae, Staphylococcus aureus, Escherichia coli, Klebsiella Pneumoniae, Pseudomonas aeruginosa, Enterobacter cloacae, Staphylococcus epidermidis, Acinetobacter baumannii, Chlamydia pneumoniae, adenovirus, respiratory syncytial virus, parainfluenza virus, influenza virus, human cytomegalovirus, human metapneumovirus and enteroviruses were provided by Benxi TestJet Biotechnologies(China). All other chemicals used in the present study were analytically pure.
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