Plp139 151 peptide

All animal studies and procedures were approved by the University of Sharjah Animal care and use committee (ACUC) and following the UoS research policy and procedures for animal care. Mice were kept under pathogen-free conditions at the University of Sharjah. Female SJL/J mice (H-2ˢ, obtained from Charles River laboratories, Fairfield, USA), ages 4–6 weeks old were immunized subcutaneously (SC) with 200 μg of PLP139–151 peptide purchased from ABBIOTEC (San Diego, CA, USA) emulsified in complete Freund’s adjuvant containing 1 mg Mycobacterium tuberculosis (Sigma-Aldrich, Sharjah branch, UAE), at four sites in the right and left flanks. Following each injection, 200 ng of Bordetella pertussis toxin (EMD chemicals, Darmstadt, Germany) was injected intraperitoneal (IP) 0 and 48 hrs after immunization with the peptide. The animals were independently observed and monitored daily, and the EAE clinical score was measured according to the following scoring scheme: 0 = no clinical disease, 1 = tail flaccidity, 2 = hind limb weakness, 3 = hind limb paralysis, 4 = forelimb paralysis, and 5 = moribund or death, as described.19 (link)

All animal studies were reviewed and approved by the University of Sharjah animal care and use committee “ACUC”; approval number UOS-ACUC-16-003, and following the UoS research policy and procedures for animal care. For in vivo experiments, female SJL/J mice (H-2ˢ, purchased from Jackson Laboratories, Bar Harbor, ME, USA), aged 4–6 weeks, were used. These mice were injected subcutaneously (SC) with 200 μg of myelin proteolipid protein “PLP” 139–151 peptide (Abbiotec, San Diego, CA, USA) emulsified in complete Freund’s adjuvant containing 1 mg Mycobacterium tuberculosis (Sigma-Aldrich, Darmstadt, Germany). A 200 ng of Bordetella pertussis toxin (Sigma-Aldrich) was injected intraperitoneally “IP” at a similar time of PLP injection, and again after 48 h. The animals were independently monitored and checked daily, and the EAE clinical score was measured according to the following scoring protocol: 0 = no clinical disease, 1 = tail flaccidity, 2 = hind limb weakness, 3 = hind limb paralysis, 4 = forelimb paralysis, and 5 = moribund or death, as described.21 (link)