Rabbit anti phospho smad3 ser423 425

Manufactured by Cell Signaling Technology

Sourced in United States

Rabbit anti-phospho-Smad3 (ser423/425) is a primary antibody that specifically recognizes Smad3 protein when phosphorylated at serine residues 423 and 425. It is designed for use in immunoassay applications to detect and quantify phosphorylated Smad3.

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Lab products found in correlation

2 protocols using rabbit anti phospho smad3 ser423 425

Ghrelin Signaling Pathway Regulation

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Rapamycin and DMSO were obtained from Sigma-Aldrich (St Louis, MO, USA). Ghrelin peptide was purchased from Phoenix Pharmaceuticals Inc. (Burlingame, CA). Rabbit anti-phospho-mTOR (ser235/236), rabbit anti-mTOR, rabbit anti-phospho-S6 (ser235/236), rabbit anti-S6, rabbit anti-phospho-AKT (ser473), rabbit anti-AKT, rabbit anti-insulin, rabbit anti-phospho-Smad2 (ser465/467), mouse anti-Smad2, rabbit anti-phospho-Smad3 (ser423/425), mouse anti-GAPDH, and mouse anti-β-actin antibodies were obtained from Cell Signaling Technology (Beverly, MA). Rabbit anti-αSMA and mouse anti-ghrelin antibodies were purchased from Abcam (Cambridge, UK). IRDye-conjugated affinity purified anti-rabbit, anti-mouse IgGs were purchased from Rockland (Gilbertsville, PA). Goat anti-rabbit fluoresceinisothiocyanate-conjugated IgG, goat anti-mouse Texas Red-conjugated IgG, and goat anti-insulin A (C-12) antibody were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Trizol reagent and the reverse transcription (RT) system were from Promega Inc. (Madison, WI).

Yu R., Li Z., Liu S., Huwatibieke B., Li Y., Yin Y, & Zhang W. (2018). Activation of mTORC1 signaling in gastric X/A-like cells induces spontaneous pancreatic fibrosis and derangement of glucose metabolism by reducing ghrelin production. EBioMedicine, 36, 304-315.

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Phosphorylation Signaling Antibody Panel

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The primary antibodies used in this study include rabbit anti-phospho-Smad3^Ser423/425 (#9520, 1:500; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-Smad3 (#9513, 1:1000; Cell Signaling Technology), rabbit anti-phospho-ezrin^Thr567 (PA5-37763, 1:500; Invitrogen, Carlsbad, CA, USA), mouse anti-ezrin (#35-7300, 1:1000; Invitrogen), rabbit anti-phospho-PKA α/β CAT (pThr197) (SAB4301240, 1:500; Sigma-Aldrich, Burlington, MA, USA), rabbit anti-PKA (06-903, 1:800; Sigma-Aldrich), rabbit anti-phospho-PKA substrates antibody (#9624, 1:1000; Cell Signaling Technology), rabbit anti-NADPH oxidase 4 (ab133303, 1:750; Abcam, Cambridge, MA, USA), and mouse anti-GAPDH (G8795, 1:10,000; Sigma-Aldrich) antibodies.

Guo W., Gao H., Pan W., Yu P, & Che G. (2021). High glucose induces Nox4 expression and podocyte apoptosis through the Smad3/ezrin/PKA pathway. Biology Open, 10(5), bio055012.

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