Sinomenine was purchased from Solarbio Life Sciences (#SS8560,Solarbio, Beijing, China), dissolved in DMSO and placed away from light at −20°C. Anti-MARCH1 antibody (#bs-9335R) was obtained from Bioss (Beijing, China). Anti-p-STAT3 (#ab32143), p-AMPK (#ab92701) and AMPK (#ab32047) antibodies were purchased from Abcam (Cambridge, United Kingdom). Anti-Bcl-2 (#12789-1-AP), CyclinB1 (#55004-1-AP), CyclinD1 (#26939-1-AP), Mcl-1 (#16225-1-AP), Bax (#50599-2-Ig), STAT3 (#10253-2-AP), GAPDH (#10494-1-AP) and Peroxidase-conjugated Affinipure Goat anti-Rabbit IgG (H + L) (#SA00001-2) antibodies were purchased from the Proteintech Group (Chicago, IL, United States).
Sinomenine
Manufactured by Solarbio
Sourced in China
Sinomenine is a laboratory equipment product manufactured by Solarbio. It is a chemical compound that can be used for various research and analytical applications. The core function of Sinomenine is to serve as a reference standard or analytical reagent in scientific experiments and studies.
Automatically generated - may contain errors
Lab products found in correlation
Mcl 1, by Proteintech (1 mentions)
Anti bcl 2, by Proteintech (1 mentions)
Peroxidase conjugated affinipure goat anti rabbit igg h l sa00001 2, by Proteintech (1 mentions)
Gapdh, by Proteintech (1 mentions)
Cyclin b1, by Proteintech (1 mentions)
Bcl2 associated x (bax), by Proteintech (1 mentions)
Cyclin d1, by Proteintech (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
2 protocols using sinomenine
1
Sinomenine Modulates STAT3 and AMPK
2
Sinomenine Impacts PDLSC Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The effects of sinomenine on the proliferation of PDLSCs were determined by Cell Counting Kit-8 (CCK-8; Dojindo Laboratories, Kumamoto, Japan). PDLSCs were plated in 96-well plates at a density of 3 × 103 cells per well. After the cell cultured in complete medium for 24 h, the medium was replaced by complete medium supplemented with 0, 0.1, 0.2, 0.5, 1.0 or 2.0 M sinomenine (Solarbio, Beijing, China) for 24, 48, and 72 hours. The cell-free group was set as the blank control. Five replicates were performed for each group. After aspirating the medium, CCK-8 was added according to the instruction (α-MEM and CCK-8 reagent were mixed at a 9:1 ratio), and the absorbance was measured at 450 nm after incubation at 37 °C in a darkroom for 2 h on a microplate reader (SPECTRAstar Nano, BMG Labtech, Ortenberg, Germany).
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