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Mouse igg1κ isotype control antibody

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The Mouse IgG1κ isotype control antibody is a laboratory reagent used to establish appropriate background staining levels in flow cytometry experiments. It serves as a negative control to differentiate specific antigen binding from non-specific binding.

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Lab products found in correlation

Decloaking chamber, by Biocare Medical (1 mentions) Diva decloaker, by Biocare Medical (1 mentions) Anti mouse nk1.1 antibody, by BioLegend (1 mentions) Nkg2d blocking antibody clone 1d11, by BioLegend (1 mentions)

Spelling variants of this product

Isotype control (56 citations) Isotype control antibody (45 citations) Mouse IgG1κ isotype (5 citations) Isotype control mouse IgG1κ (2 citations) Mouse IgG1κ isotype control antibody (MOPC21) (2 citations)

4 protocols using mouse igg1κ isotype control antibody

1

Immunohistochemical Staining of Formalin-Fixed Tissues

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Tissues obtained from US Biomax (Derwood, MD) were formalin-fixed, paraffin embedded, and sectioned at 5 μm. The sections were then deparaffinized, rehydrated in water, and prepared by heat-induced antigen retrieval by Diva Decloaker (Biocare Medical Cat. No. DV2004MX), and Decloaking Chamber (Biocare Medical). Sections were stained with Bsg (1 mg/mL, 1:500) or Mouse IgG1κ isotype control antibody (Biolegend Cat. No. 401407, 1:500) by using PowerVision+ poly-HRP IHC detection systems (Leica Cat. No.PV6106).
White J.M., Kuda-Wedagedara A.N., Wicker M.N., Spratt D.E., Schopperle W.M., Heath E, & Viola N.T. (2020). Detecting TRA-1–60 in Cancer via a Novel Zr-89 Labeled ImmunoPET Imaging Agent. Molecular pharmaceutics, 17(4), 1139-1147.
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2

SSEA-5 Antibody Labeling of Qβ VLPs

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Qβ(ZZ)@EGFP or Qβ(ZZ)@CD VLPs were labeled with anti-SSEA-5 antibodies. Briefly, VLPs were diluted to 0.1 mg/mL and 20 μg VLP was incubated with 50 μg purified anti-human SSEA-5 mouse IgG1, κ antibody (Clone 8e11, BioLegend) for 2 h at 37°C. A antibody:ZZ-domain mole ratio of 0.75 was used for all labeling reactions and was sufficient for uniform labeling of the particles. To serve as a control, the same procedure was repeated with purified mouse IgG1, κ isotype control antibody (Clone MG1–45, BioLegend).
Rampoldi A., Crooke S.N., Preininger M.K., Jha R., Maxwell J., Ding L., Spearman P., Finn M.G, & Xu C. (2018). Targeted Elimination of Tumorigenic Human Pluripotent Stem Cells Using Suicide-inducing Virus-like Particles. ACS chemical biology, 13(8), 2329-2338.
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3

NK-1.1 Antibody Coating Assay

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A 96-well flat bottom plate was coated with 5 μg/ml purified anti-mouse NK-1.1 antibody (BioLegend) or 5 μg/ml purified Mouse IgG1, κ, Isotype Control Antibody (BioLegend) incubated for 1 day. The supernatant was then removed, and cells were cultured for a week.
Wada H., Shibata Y., Abe Y., Otsuka R., Eguchi N., Kawamura Y., Oka K., Baghdadi M., Atsumi T., Miura K, & Seino K.I. (2019). Flow cytometric identification and cell-line establishment of macrophages in naked mole-rats. Scientific Reports, 9, 17981.
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4

NKG2D Blocking Antibody Assay

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NKG2D blocking antibody (clone 1D11) and mouse IgG1κ isotype control antibody were purchased from BioLegend (San Diego, CA, USA). T cells and NK cells were incubated with the blocking antibody (5 μg/mL) for 30 min at room temperature. The blocking and isotype control antibodies were maintained at 5 μg/mL in the killing assay medium.
Li S., Zhao R., Zheng D., Qin L., Cui Y., Li Y., Jiang Z., Zhong M., Shi J., Li M., Wang X., Tang Z., Wu Q., Long Y., Hu D., Wang S., Yao Y., Liu S., Yang L.H., Zhang Z., Tang Q., Liu P., Li Y, & Li P. (2022). DAP10 integration in CAR-T cells enhances the killing of heterogeneous tumors by harnessing endogenous NKG2D. Molecular Therapy Oncolytics, 26, 15-26.
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