For immunophenotyping of cell surface antigens, third-passage PD-MSCsPRL-1 were detached, stained with antibodies conjugated with fluorescein isothiocyanate (FITC) and phycoerythrin (PE) and analyzed with a FACSCalibur flow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA). The following monoclonal antibodies were used: CD34-PE, CD90-PE, HLA-ABC-FITC, HLA-DR-FITC (BD Bioscience, San Jose, CA, USA), CD13-PE (BioLegend, San Diego, CA, USA), CD105-FITC (R&D Systems, Minneapolis, MN, USA), and HLA-G (Abcam, Cambridge, UK). For each sample, at least 10,000 events were acquired.
Cd13 pe
Manufactured by BioLegend
Sourced in United States
CD13-PE is a fluorescently labeled antibody that binds to the CD13 antigen, also known as aminopeptidase N. CD13 is expressed on the surface of various cell types, including myeloid cells, endothelial cells, and epithelial cells. The PE (phycoerythrin) fluorescent label allows for the detection and analysis of CD13-positive cells using flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
Hla g, by Abcam (6 mentions)
Cd34 pe, by BD (5 mentions)
Cd90 pe, by BD (5 mentions)
Hla dr fitc, by BD (5 mentions)
Hla abc fitc, by BD (5 mentions)
Cd105 fitc, by R&D Systems (5 mentions)
Facscalibur ow cytometer, by BD (5 mentions)
Facscalibur flow cytometer, by BD (4 mentions)
Hla abc, by BD (4 mentions)
Cd105 pe, by R&D Systems (4 mentions)
Hla g fitc, by Abcam (3 mentions)
Appropriate isotype antibodies, by BD (2 mentions)
Hla dr, by BD (2 mentions)
Isotype antibodies, by BD (2 mentions)
9 protocols using cd13 pe
1
Immunophenotyping of PD-MSCs
2
Phenotyping Mesenchymal Stem Cells
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PD-MSCsPRL-1 (5 passages) were stained with monoclonal antibodies specific for the following proteins to phenotype cell-surface antigens: CD34 (PE), CD90 (PE), HLA-ABC (FITC), HLA-DR (FITC) (BD Bioscience, San Diego, CA, USA), CD13 (PE) (BioLegend, San Diego, CA, USA), CD105 (PE) (R&D Systems, Minneapolis, MN, USA), and HLA-G (FITC) (Abcam). After staining, cells were washed in PBS and treated with appropriate isotype antibodies (BD Biosciences, San Jose, CA, USA). Cells were analyzed by a FACSCalibur flow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA). For each sample, at least 10,000 events were acquired.
3
Immunophenotyping of PD-MSCs
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For immunophenotyping of cell surface antigens, PD-MSCs PEDF (passage = 3) were stained with fluorescein isothiocyanate (FITC)-and phycoerythrin (PE)-conjugated antibodies. Antibodies against CD34-PE, CD90-PE, HLA-ABC-FITC, HLA-DR-FITC (BD Bioscience, San Jose, CA, USA), CD13-PE (BioLegend, San Diego, CA, USA), CD105-FITC (R&D Systems, Minneapolis, MN, USA), and HLA-G-FITC (Abcam, Cambridge, UK) were analyzed with a FACSCalibur flow cytometer (Becton Dickinson, Franklin Lakes, NJ). A total of 10,000 events were acquired. All reactions were analyzed at least in triplicate.
4
PD-MSCs surface antigen profiling
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PD-MSCs PRL-1 (5 passages) were stained with monoclonal antibodies specific for the following proteins to phenotype cell-surface antigens: CD34 (PE), CD90 (PE), HLA-ABC (FITC), HLA-DR (FITC) (BD Bioscience, San Diego, CA, USA), CD13 (PE) (BioLegend, San Diego, CA, USA), CD105 (PE) (R&D Systems, Minneapolis, MN, USA), and HLA-G (FITC) (Abcam). After staining, cells were washed in PBS and treated with appropriate isotype antibodies (BD Biosciences, San Jose, CA, USA). Cells were analyzed by a FACSCalibur flow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA). For each sample, at least 10,000 events were acquired.
5
Cell Surface Antigen Immunophenotyping of PD-MSCs
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For immunophenotyping of cell surface antigens, third-passage PD-MSCs PRL-1 were detached, stained with antibodies conjugated with uorescein isothiocyanate (FITC) and phycoerythrin (PE) and analyzed with a FACSCalibur ow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA). The following monoclonal antibodies were used: CD34-PE, CD90-PE, HLA-ABC-FITC, HLA-DR-FITC (BD Bioscience, San Jose, CA, USA), CD13-PE (BioLegend, San Diego, CA, USA), CD105-FITC (R&D Systems, Minneapolis, MN, USA), and HLA-G (Abcam, Cambridge, UK). For each sample, at least 10,000 events were acquired.
6
Cell Surface Antigen Immunophenotyping of PD-MSCs
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For immunophenotyping of cell surface antigens, third-passage PD-MSCs PRL-1 were detached, stained with antibodies conjugated with uorescein isothiocyanate (FITC) and phycoerythrin (PE) and analyzed with a FACSCalibur ow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA). The following monoclonal antibodies were used: CD34-PE, CD90-PE, HLA-ABC-FITC, HLA-DR-FITC (BD Bioscience, San Jose, CA, USA), CD13-PE (BioLegend, San Diego, CA, USA), CD105-FITC (R&D Systems, Minneapolis, MN, USA), and HLA-G (Abcam, Cambridge, UK). For each sample, at least 10,000 events were acquired.
7
Phenotyping PD-MSCs Using Flow Cytometry
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PD-MSCs PRL-1 (5 passages) were stained with monoclonal antibodies speci c for the following proteins to phenotype cell-surface antigens: CD34 (PE), CD90 (PE), HLA-ABC (FITC), HLA-DR (FITC) (BD Bioscience, San Diego, CA, USA), CD13 (PE) (BioLegend, San Diego, CA, USA), CD105 (PE) (R&D Systems, Minneapolis, MN, USA), and HLA-G (FITC) (Abcam). After staining, cells were washed in PBS and treated with appropriate isotype antibodies (BD Biosciences, San Jose, CA, USA). Cells were analyzed by a FACSCalibur ow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA). For each sample, at least 10,000 events were acquired.
8
Cell Surface Antigen Immunophenotyping of PD-MSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For immunophenotyping of cell surface antigens, third-passage PD-MSCs PRL-1 were detached, stained with antibodies conjugated with uorescein isothiocyanate (FITC) and phycoerythrin (PE) and analyzed with a FACSCalibur ow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA). The following monoclonal antibodies were used: CD34-PE, CD90-PE, HLA-ABC-FITC, HLA-DR-FITC (BD Bioscience, San Jose, CA, USA), CD13-PE (BioLegend, San Diego, CA, USA), CD105-FITC (R&D Systems, Minneapolis, MN, USA), and HLA-G (Abcam, Cambridge, UK). For each sample, at least 10,000 events were acquired.
9
Phenotyping PD-MSCs Using Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PD-MSCs PRL-1 (5 passages) were stained with monoclonal antibodies speci c for the following proteins to phenotype cell-surface antigens: CD34 (PE), CD90 (PE), HLA-ABC (FITC), HLA-DR (FITC) (BD Bioscience, San Diego, CA, USA), CD13 (PE) (BioLegend, San Diego, CA, USA), CD105 (PE) (R&D Systems, Minneapolis, MN, USA), and HLA-G (FITC) (Abcam). After staining, cells were washed in PBS and treated with appropriate isotype antibodies (BD Biosciences, San Jose, CA, USA). Cells were analyzed by a FACSCalibur ow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA). For each sample, at least 10,000 events were acquired.
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