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Fully motorized rotary microtome

Manufactured by Leica
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The Fully motorized rotary microtome is a precision instrument designed for the sectioning of biological samples. It features a motorized rotation mechanism that allows for controlled and consistent cutting of specimens, ensuring accurate and reproducible sections.

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Microtome (1520 citations) Rotary microtome (327 citations)

3 protocols using fully motorized rotary microtome

1

Tissue Fixation and Sectioning Protocol

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Embryos and tissues samples were fixed in 2% paraformaldehyde at 4°C overnight, ethanol dehydrated, embedded in paraffin, and sectioned at 6- to 8-μm thickness using a Leica fully motorized rotary microtome.
Janardhan H.P., Milstone Z.J., Shin M., Lawson N.D., Keaney JF J.r, & Trivedi C.M. (2017). Hdac3 regulates lymphovenous and lymphatic valve formation. The Journal of Clinical Investigation, 127(11), 4193-4206.
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2

Tissue Fixation and Sectioning Protocol

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Tissue samples were collected, fixed in 4% paraformaldehyde in 1× phosphate-buffered saline (PBS) at 4°C overnight, dehydrated through an ethanol gradient, embedded in paraffin, and sectioned at 8-μm thickness on a Leica fully motorized rotary microtome.
Milstone Z.J., Saheera S., Bourke L.M., Shpilka T., Haynes C.M, & Trivedi C.M. (2020). Histone deacetylases 1 and 2 silence cryptic transcription to promote mitochondrial function during cardiogenesis. Science Advances, 6(15), eaax5150.
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3

Histological processing and staining of murine tissues

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Murine embryos and tissues were fixed in 2% paraformaldehyde at 4°C overnight, ethanol dehydrated, embedded in paraffin, and sectioned at 8-µm thickness using a Leica fully motorized rotary microtome. FFPE tissue sections were deparaffinized in xylene and rehydrated through an ethanol gradient, followed by 2-min Harris-modified hematoxylin and 30-s eosin-Y staining. Slides were dehydrated with ethanol, cleared with xylene, and mounted with Vectashield glass mounting medium.
Janardhan H.P., Meng X., Dresser K., Hutchinson L, & Trivedi C.M. (2020). KRAS or BRAF mutations cause hepatic vascular cavernomas treatable with MAP2K–MAPK1 inhibition. The Journal of Experimental Medicine, 217(7), e20192205.
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