Antibodies against reticulon 4B (AB-163; Kinasource Ltd, Dundee, UK), reticulon 4A (ab62024; Abcam, Cambridge, UK), HA (MMS-101R-50; Covance, Princeton, NJ), FLAG (F7425; Sigma-Aldrich), calreticulin (2679S; Cell Signalling Technologies, MA) and β-actin (ab8227-50; Abcam) were used as primary antibodies. When indicated, rabbit anti-sheep bridging antibody (313-001-003; Jackson ImmunoResearch Labs Inc., West Grove, PA) was used. Secondary antibodies were Rhodamine Red-X (016-290-084; Jackson ImmunoResearch), Alexa 647 (A31571; Life technologies), Alexa 488 (A-11008; Life Technologies) and 1.4 nm nanogold-conjugated anti-rabbit antibody (Nanoprobes, Stony Brook, NY).
Ab8227 50
Manufactured by Abcam
Ab8227-50 is a lab equipment product offered by Abcam. It is a core component used for a specific function in research or laboratory settings. However, without access to the detailed product information, I cannot provide a more specific and unbiased description while maintaining conciseness. The core function of this product is not available for me to present in a factual manner without the risk of extrapolation or interpretation.
Automatically generated - may contain errors
Lab products found in correlation
Calreticulin, by Cell Signaling Technology (1 mentions)
Ab62024, by Abcam (1 mentions)
Alexa 647, by Thermo Fisher Scientific (1 mentions)
Rhodamine red x, by Jackson ImmunoResearch (1 mentions)
Alexa 488, by Thermo Fisher Scientific (1 mentions)
F7425, by Merck Group (1 mentions)
Ha mms 101r 50, by Fortrea (1 mentions)
Rad50, by Cell Signaling Technology (1 mentions)
Dna pk, by Cell Signaling Technology (1 mentions)
Enhanced chemiluminescence ecl detection system, by GE Healthcare (1 mentions)
Bca kit, by Thermo Fisher Scientific (1 mentions)
β actin, by Abcam (1 mentions)
2 protocols using ab8227 50
1
Immunofluorescence and Immunogold Labeling
2
Western Blot Analysis of DNA Damage Signaling
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Protein concentrations were determined using the BCA kit (Pierce Biotechnology, Rockford, IL) according to the manufacturer's instructions. Protein lysates (20-100 μg) were electrophoretically resolved by SDS/PAGE, transferred to nitrocellulose membrane, and probed with the indicated primary antibodies: Phospho-ATM (Ser1981) (D6H9) from rabbit (1:500, 5883, Cell Signaling, Danvers, MA), Phospho-BRCA1 (Ser1524) from rabbit (1:500, 9009, Cell Signaling), DNA-PK from rabbit (1:500, 4602, Cell Signaling), Mre11 (31H4) from rabbit (1:500, 4847, Cell Signaling) and Rad50 from rabbit (1:500, 3427, Cell Signaling). Membranes were then incubated with a 1:5,000 dilution of a peroxidase conjugated corresponding secondary antibody. Equal loading of the protein samples was confirmed by parallel western blots for β-actin (1:5,000, ab822750; Abcam). Detection was performed using the ECL-Enhanced Chemiluminescence Detection System (GE Healthcare Biosciences, Pittsburgh, PA) according to the manufacturer's instructions. Blots were visualized by autoradiography.
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