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Human gapdh

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GAPDH is an enzyme involved in the glycolysis pathway, which is responsible for the breakdown of glucose to produce energy. The human GAPDH product provides a reliable and consistent source of this important protein for use in various laboratory applications.

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Lab products found in correlation

Quantifast sybr green rt pcr kit, by Qiagen (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Quantitect reverse transcriptase kit, by Qiagen (1 mentions) Direct zol rna miniprep plus kit, by Zymo Research (1 mentions) Lightcycler 480 sybr green, by Roche (1 mentions)

Spelling variants of this product

GAPDH (170 citations) RT2 qPCR Primer Assays for Human GAPDH and ABAT (2 citations) RT2 qPCR Primer Assay for Human GAPDH (2 citations)

2 protocols using human gapdh

1

Quantitative RT-PCR of GAPDH and MTSS1

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RNA was harvested with Trizol (Life Technologies), resuspended in RNAse-free water, and analyzed for purity by 260/280 absorbance via Nanodrop. RT-PCR was conducted on the BioRad-CFX-Connect cycler using Qiagen QuantiFast SYBR Green RT-PCR kit (Qiagen 204154) and the following primers: Human GAPDH (Qiagen PPH00150F) and Human MTSS1 (Qiagen PPH10073B).
Zeleniak A.E., Huang W., Fishel M.L, & Hill R. (2017). PTEN-Dependent Stabilization of MTSS1 Inhibits Metastatic Phenotype in Pancreatic Ductal Adenocarcinoma. Neoplasia (New York, N.Y.), 20(1), 12-24.
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2

Quantification of Gene Expression in EBV B Cells

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Total RNA was isolated from EBV B cells carrying the UBE2L3-YDJC non-risk or risk haplotype using Direct-zol RNA MiniPrep Plus kit (Zymo Research) according to the manufacturer’s protocol. cDNA synthesis was performed using QuantiTect reverse transcriptase kit (Qiagen) as per the manufacturer’s recommendations. Gene expression was measured by real-time PCR analysis using Light Cycler480 SYBR Green (Roche Diagnostics). Gene expression primers for human UBE2L3, human YDJC, human SDF2L1, and human PPIL2 were purchased from Integrated DNA Technologies (Supplemental Table 5); human GAPDH (#PPH00150F) was from Qiagen.
Gopalakrishnan J., Tessneer K.L., Fu Y., Pasula S., Pelikan R.C., Kelly J.A., Wiley G.B, & Gaffney P.M. (2021). Variants on the UBE2L3-YDJC autoimmune disease risk haplotype increase UBE2L3 gene expression by modulating CTCF and YY1 binding.. Arthritis & rheumatology (Hoboken, N.J.), 74(1), 163-173.
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