H1581

Manufactured by American Type Culture Collection

Sourced in United States

The H1581 is a laboratory equipment product provided by the American Type Culture Collection (ATCC). It serves as a core function for laboratory applications, but a detailed unbiased description is not available at this time.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions) H1299, by American Type Culture Collection (5 mentions) H1993, by American Type Culture Collection (4 mentions) Sk mes 1, by American Type Culture Collection (4 mentions) Hcc95, by American Type Culture Collection (3 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) H1581, by Thermo Fisher Scientific (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Azd6244, by Selleck Chemicals (2 mentions) Azd4547, by AstraZeneca (2 mentions) H1703, by American Type Culture Collection (2 mentions) Sh circ 0020123, by GenePharma (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Sh nc, by GenePharma (1 mentions) Pcdna3 vector, by GenePharma (1 mentions) Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions) Pcdna3.1 vector, by RiboBio (1 mentions) Snu475, by American Type Culture Collection (1 mentions) H1993, by Thermo Fisher Scientific (1 mentions) Pcdna3.1 backbone vector, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

NCI-H1581 (6 citations)

21 protocols using h1581

NSCLC Tissue Sampling and Cell Culture

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NSCLC (cancer) and non-cancer tissues were obtained from each patient during biopsy. Weight of tissues ranged from 0.05 to 0.11g. All tissues were confirmed by at least three pathologists.
Human NSCLC cell lines H1581 and H1993 (ATCC, USA) were used in this study. RPMI-1640 medium (10% FBS) was used to cultivate cells. Cell culture conditions were 37 °C and 5% CO₂.

Xu S., Zhai S., Du T, & Li Z. (2020). LncRNA MIR503HG Inhibits Non-Small Cell Lung Cancer Cell Proliferation by Inducing Cell Cycle Arrest Through the Downregulation of Cyclin D1. Cancer Management and Research, 12, 1641-1647.

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Silencing Circular RNA in NSCLC Cells

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Human NSCLC cells (H1299, H1581 and A549) (ATCC, Manassas, VA, USA) and bronchial epithelial cells (16HBE) (Procell Life Science & Technology, Wuhan, China) were maintained in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS, Gibco) and 1% Penicillin-Streptomycin (Gibco). All the cells were cultured at 37°C with 5% CO₂. After the cell density reached 50–60% in 6-well plates, cell transfection could be carried out. The small interfering RNA (siRNA) of circ_0020123 (si-circ_0020123#1: 5ʹ-ATGACCAGCTTACGTTGAATT-3ʹ; si-circ_0020123#2: 5ʹ-ACCAGCTTACGTTGAATTAAT-3ʹ; si-circ_0020123#3: 5ʹ-GACCAGCTTACGTTGAATTAA-3ʹ) and zinc-finger protein X-linked (ZFX) (si-ZFX#1, F 5ʹ-UUACAUAGCGAAAAUCGGCCC-3ʹ, R 5ʹ-GCCGAUUUUCGCUAUGUAAAU-3ʹ; si-ZFX#2, F 5ʹ-UUUACAUAGCGAAAAUCGGCC-3ʹ, R 5ʹ-CCGAUUUUCGCUAUGUAAAUA-3ʹ; si-ZFX#3, F 5ʹ-AUUUACAUAGCGAAAAUCGGC-3ʹ, R 5ʹ-CGAUUUUCGCUAUGUAAAUAU-3ʹ) or their negative control (si-NC), miR-142-3p mimic and inhibitor (miR-142-3p and in-miR-142-3p) or their negative control (miR-NC and in-miR-NC), as well as the lentivirus short hairpin RNA (shRNA) of circ_0020123 (sh-circ_0020123) and its control (sh-NC) were constructed by GenePharma (Shanghai, China). The oligos (50 nM) were transfected into cells using Lipofectamine 3000 Reagent (Invitrogen, Carlsbad, CA, USA) referring to the manufacturer’s protocol.

Lu J., Ma X., Lin J, & Hou P. (2021). Circ_0020123 Increases ZFX Expression to Facilitate Non-Small Cell Lung Cancer Progression by Sponging miR-142-3p. Cancer Management and Research, 13, 1687-1698.

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NSCLC Cell Line Overexpression Assay

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Two human NSCLC cell lines H1993 and H1581 (ATCC, USA) were used. RPMI-1640 medium (90%) was mixed with FBS (10%) to grow cells. Cells were grown at 37 °C in an incubator with 95% humidity and 5% CO2. WT1-AS or UCA1 overexpression vector was constructed with pcDNA3 vector (GenePharma, Shanghai, China). At the confluence of 70–90%, H1993, and H1581 cells were harvested and transfected with either 10 nM WT1-AS or UCA1 overexpression vector. Negative control (NC) experiment was performed by transfecting empty vector into the same number of cells. To perform Control (C) cells, cells without transfections were grown until the end of experiments. After cell transfections, cells were cultivated under normal conditions for another 24 h.

Wan Y., Yao D., Fang F., Wang Y., Wu G, & Qian Y. (2021). LncRNA WT1-AS downregulates lncRNA UCA1 to suppress non-small cell lung cancer and predicts poor survival. BMC Cancer, 21, 104.

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NSCLC Cell Lines for Biopsy Analysis

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All patients received lung biopsy, and the tumor tissues and adjacent healthy tissues (about 0.1 g) were obtained from each patient. NSCLC cell lines H1581 and H1993 (ATCC, USA) were used. H1581 cell line was from a 44 years old male and H1993 cell line was from a 47 years old female. These two cell lines were used to match the patients. The cell culture medium was composed of 90% RPMI-1640 medium and 10% fetal bovine serum (FBS). Cells were cultivated at 37 °C with 5% CO₂.

Zhang L., Jin C., Yang G., Wang B., Hua P, & Zhang Y. (2020). LncRNA WTAPP1 promotes cancer cell invasion and migration in NSCLC by downregulating lncRNA HAND2-AS1. BMC Pulmonary Medicine, 20, 153.

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NSCLC Tissue Collection and Cell Culture

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Fifty two pairs of NSCLC tissues and corresponding adjacent normal tissues were resected from NSCLC patients enrolled at the First Affiliated Hospital of Zhengzhou University from 2013–2015 and preserved at – 80 ℃. All patients were chosen based on the guidelines supplied by World Health Organization (WHO) and the International Association for the Study of Lung Cancer (IASLC) [24 (link)]. Follow-up of these 52 patients was implemented from date of surgery until end of this study or death. All participators signed informed consent.
Human bronchial epithelial cells 16HBE (CL-0249; Procell, Wuhan, China) and NSCLC cells H1299 (ATCC^® CRL-5803D; ATCC, Manassas, VA, USA), A549 (ATCC^® CCL-185), H1581 (ATCC^® CRL-5878) and H23 (ATCC^® CRL-5800) were cultured in Dulbecco’s Modified Eagle Medium (Gibco, Grand Island, NY, USA) mixed with 10% fetal bovine serum (Gibco) and 1% penicillin/streptomycin (Gibco) in a humidified incubator at 37 ℃ containing 5% CO₂.

Zhang F., Cheng R., Li P., Lu C, & Zhang G. (2021). Hsa_circ_0010235 functions as an oncogenic drive in non-small cell lung cancer by modulating miR-433-3p/TIPRL axis. Cancer Cell International, 21, 73.

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NSCLC Cell Line Cultivation

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H650 and H1581 (ATCC, USA) human NSCLC cell lines were used. A mixture containing 5% FBS and 85% RPMI-1640 Medium was used as cell culture medium. Cells were cultivated under conditions of 37 °C and 5% CO₂.

Xia H., Xiu M., Gao J, & Jing H. (2019). LncRNA PLAC 2 downregulated miR-21 in non-small cell lung cancer and predicted survival. BMC Pulmonary Medicine, 19, 172.

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Regulation of HCC Cells by TINCR and ROCK1

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In vitro experiments in this study were performed using HCC cell lines H1581 (Cat# ATCC® CRL-5878™) and SNU-475 (ATCC® CRL-2236™) from ATCC (USA). Cells were cultivated at 37 °C with 5% CO₂ in RPMI-1640 medium (10% FBS) before use. PcDNA3.1 vectors expressing TINCR and ROCK1 were constructed by RIBOBIO (Guangzhou, China). MiR-214-5p mimic and negative control miRNA were also from RIBOBIO. H1581 cells were harvested and were counted, followed by transfection of 10 nM pcDNA3.1 vector expressing TINCR or ROCK2, or 10 nM empty pcDNA3.1 vector (negative control, NC), or 30 nM miR-214-5p mimic, or 30 nM negative control miRNA (NC) into 10⁶ cells through lipofectamine 2000 (Invitrogen, USA)-mediated method. Cells with no transfections were also included to serve was control (C) group. The interval between subsequent experiments and transfection was 24 h.

Hu M., Han Y., Zhang Y., Zhou Y, & Ye L. (2020). lncRNA TINCR sponges miR-214-5p to upregulate ROCK1 in hepatocellular carcinoma. BMC Medical Genetics, 21, 2.

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NSCLC Tissue and Cell Line Experiments

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Paired NSCLC and non-tumor lung tissues were obtained from each patient through biopsy. All tissue samples were confirmed by histopathological analysis and were stored in a liquid nitrogen tank before use. Two NSCLC cell lines, H1581 and H1993, were purchased from ATCC (USA). Cells were cultivated in a 5% CO2 incubator at 37 °C. Cell culture medium was composed of RPMI-1640 medium (90%) and FBS (10%). SNHG9 expression vector was constructed using pcDNA3.1 backbone vector (Invitrogen). Mimic of miR-21 and control miRNA were provided by Sangon (Shanghai, China). H1581 and H1993 cells were harvested at about 85% confluence, followed by the transfection of cells with either vector (10 nM) or miRNA (40 nM) using Lipofectamine 2000 (Invitrogen). PcDNA3.1 empty vector or control miRNA-transfected cells were annotated as negative control (NC) group. Untransfected cells were used as control (C). Subsequent analyses were performed at 48 h post-transfection. Lipofectamine 2000 (Invitrogen) was used as transfection reagent.

Wang D., Cao X., Han Y, & Yu D. (2020). LncRNA SNHG9 is Downregulated in Non-Small Cell Lung Cancer and Suppressed miR-21 Through Methylation to Promote Cell Proliferation. Cancer Management and Research, 12, 7941-7948.

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Culturing NSCLC Cell Lines

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The human NSCLC cell line PC‐9 was provided by Tokyo Medical University (Tokyo, Japan),21, 22 and the LK‐2, A549, H520, H1299, and H1581 lines were obtained from ATCC (Manassas, VA, USA) and authenticated by short tandem repeat‐based DNA profiling (Takara Bio, Shiga, Japan). All cells were cultured under a humidified atmosphere of 5% CO₂ at 37°C in RPMI‐1640 (Sigma, St. Louis, MO, USA) supplemented with 10% heat‐inactivated FBS (Equitech‐Bio, Kerrville, TX, USA).

Hibi M., Kaneda H., Tanizaki J., Sakai K., Togashi Y., Terashima M., De Velasco M.A., Fujita Y., Banno E., Nakamura Y., Takeda M., Ito A., Mitsudomi T., Nakagawa K., Okamoto I, & Nishio K. (2016). FGFR gene alterations in lung squamous cell carcinoma are potential targets for the multikinase inhibitor nintedanib. Cancer Science, 107(11), 1667-1676.

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Establishing Stable Target Cell Lines

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All of the cell lines were monitored regularly for mycoplasma contamination using an in-house PCR assay.⁴³ (link) In preparation for the cell assays, healthy cultures of Jurkat E6-1 cells (catalog no. TIB-152, American Type Culture Collection [ATCC]#) were maintained in RPMI complete (RPMI 1640 supplemented with 10% fetal bovine serum [FBS], 2 mM l-glutamine, 1 mM sodium pyruvate, and 100 μg/mL penicillin/streptomycin), with cell densities between 0.25 and 1 M cells/mL for several weeks; we have found this to be critical for consistent results in Jurkat activation assays. All of the target cell lines described in this paper were modified using the Nuclight-Red Lentiviral reagent (catalog no. 4625, Sartorius) to generate stable red fluorescent cells, which can be easily differentiated from effector cells in flow cytometry or live microscopy analyses. Specific target lines used were as follows: Raji (catalog no. CCL-86, ATCC), Ramos (catalog no. CRL-1596, ATCC), SKOV3 (catalog no. HTB-77, ATCC), MCF7 (catalog no. HTB-22, ATCC), H1581 (catalog no. CRL-5878, ATCC). Target cell lines were cultured in varying media conditions, as recommended by the ATCC cell repository.

McComb S., Arbabi-Ghahroudi M., Hay K.A., Keller B.A., Faulkes S., Rutherford M., Nguyen T., Shepherd A., Wu C., Marcil A., Aubry A., Hussack G., Pinto D.M., Ryan S., Raphael S., van Faassen H., Zafer A., Zhu Q., Maclean S., Chattopadhyay A., Gurnani K., Gilbert R., Gadoury C., Iqbal U., Fatehi D., Jezierski A., Huang J., Pon R.A., Sigrist M., Holt R.A., Nelson B.H., Atkins H., Kekre N., Yung E., Webb J., Nielsen J.S, & Weeratna R.D. (2024). Discovery and preclinical development of a therapeutically active nanobody-based chimeric antigen receptor targeting human CD22. Molecular Therapy Oncology, 32(1), 200775.

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