Pf4708671

Manufactured by Santa Cruz Biotechnology

Sourced in United States

PF4708671 is a small molecule inhibitor. It functions by targeting and inhibiting a specific enzyme in cellular pathways.

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Lab products found in correlation

3 protocols using pf4708671

Ligands and Inhibitors for Cell Signaling

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Des[1–3]IGF-1 and recombinant human epidermal growth factor (EGF) were obtained from Cell Sciences (Canton, MA). alamarBlue, Dulbecco modified Eagle’s medium (DMEM)/F-12 media with HEPES, Roswell Park Memorial Institute (RPMI) 1640 media, and fetal bovine serum were obtained from Invitrogen (Carlsbad, CA). Hydrocortisone was purchased from Sigma–Aldrich (St. Louis, MO). OSI-906 and BMS754807 were obtained from Chemietek (Indianapolis, IN). Gefitinib, lapatinib, crizotinib, and cederanib were obtained from LC Laboratories (Woburn, MA). Dasatinib, BMS599626, NVP-BEZ235, PF-04691502, XL-147, MKK2206, GSK690693, temsirolimus, AT7867, foretinib, panobinostat, SAHA, diclofenac, serdemetan, and YM-155 were obtained from Selleck Chemicals (Houston, TX). PF-4708671 was obtained from Santa Cruz Biotechnology (Dallas, TX). PP242 was kindly provided by Dr. Michael Harding (University of Virginia, Charlottesville, VA). Anti-IGF-1Rβ, anti-AKT, anti-pAKT (S473), anti-pAKT (T308), anti-EGFR, and anti-pEGFR, were obtained from Cell Signaling Technology (Beverly, MA). Anti-pIGF-1R/pIR (Y1158/Y1162/Y1163) was obtained from Millipore (Bill-erica, MA).

Axelrod M.J., Mendez R.E., Khalil A., Leimgruber S.S., Sharlow E.R., Capaldo B., Conaway M., Gioeli D.G., Weber M.J, & Jameson M.J. (2015). Synergistic apoptosis in head and neck squamous cell carcinoma cells by co-inhibition of insulin-like growth factor-1 receptor signaling and compensatory signaling pathways. Head & neck, 37(12), 1722-1732.

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Insulin and Doxorubicin Cytotoxicity Pathway

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Insulin, human recombinant from Saccharomyses cerevisiae, was purchased from Sigma-Aldrich. Doxorubicin was obtained from Tocris. Anti-survivin, anti-caspase-3 (cleaved form), anti-phospho-p53 (Ser¹⁵), anti-phospho-Akt (Ser⁴⁷³), anti-Akt, anti-phospho-mTOR (Ser²⁴⁸¹) and anti-mTOR antibodies were obtained from Cell Signaling. Anti-Sp1, anti-phospho-p70S6K (Thr⁴²¹/Ser⁴²⁴), anti-p70S6K, anti-β-actin, anti-GAPDH, anti-Smac/DIABLO antibodies and p70S6K inhibitor PF4708671 were purchased from Santa Cruz Biotechnology. Anti-VDAC1, anti-p53, anti-Bcl-2, and anti-BAX antibodies were obtained from Abcam. PI3K inhibitor LY294002 and mTOR inhibitor Rapamycin were purchased from Calbiochem. Anti-cytochrome c antibody was included in ApoAlert Cell Fractionation Kit (Clontech).

Lee B.S., Oh J., Kang S.K., Park S., Lee S.H., Choi D., Chung J.H., Chung Y.W, & Kang S.M. (2015). Insulin Protects Cardiac Myocytes from Doxorubicin Toxicity by Sp1-Mediated Transactivation of Survivin. PLoS ONE, 10(8), e0135438.

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Characterization of High-Grade Serous OC Cell Lines

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The high-grade serous OC cell lines PEA1, PEO1 and PEO14 have been described elsewhere.^{42 (link)} Cell line verification was performed by Identifiler kit (Applied Biosystems-Thermo Fisher Scientific, Waltham, MA, USA). All lines were maintained in RPMI 1640 media with 10% fetal bovine serum, penicillin, streptomycin, glutamine at 37 °C/5% CO₂. Cell lines are routinely monitored in our laboratory by microscopic morphology check.
For wound-healing and gene expression experiments, cells were treated with the selective p70S6K inhibitor PF4708671 (Santa Cruz Biotechnology, Dallas, TX, USA, sc-361288), dissolved in DMSO.

Amoroso M.R., Matassa D.S., Agliarulo I., Avolio R., Lu H., Sisinni L., Lettini G., Gabra H., Landriscina M, & Esposito F. (2016). TRAP1 downregulation in human ovarian cancer enhances invasion and epithelial–mesenchymal transition. Cell Death & Disease, 7(12), e2522-.

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