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Crystal violet reagent

Manufactured by Roche
Sourced in Germany

Crystal Violet reagent is a laboratory stain used for various applications, including cell and tissue staining. It is a basic dye that binds to negatively charged molecules, such as DNA and RNA, producing a distinct purple color. The reagent is commonly used in microbiology, histology, and cytology for the identification and visualization of cellular structures.

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2 protocols using crystal violet reagent

1

Apoptosis Evaluation Protocol

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All antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Crystal Violet reagent, RPMI-1640, fetal bovine serum (FBS), Annexin V-FLUOS Staining kit, Propidium Iodide (PI), and RNase A were purchased from Roche (Manheim, Germany).
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2

Cytotoxicity Assay for Cancer Cells

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Roswell Park Memorial Institute medium (RPMI-1640, BIO-L500-500) and FBS (fetal bovine serum, BIO-S1650-500) and Trypsin (BIO-L0931-100) were obtained from Biowest company (Riverside, MO, USA). Dimethyl sulfoxide (D4540-100), Poly-l-lisyne (P8920-100) and Cisplatin (cis-Diammineplatinum (II) dichloride, 479306-1G) were obtained from Sigma-Aldrich (St. Louis, MO, USA). TUNEL staining kit was acquired from Promega Corporation (Madison, WI, USA). Proteinase K (IB05406) was obtained from IBI Scientific (Kapp Ct, IA, USA). The antibodies anti-PCNA (sc-9857) and Ki67 (sc-15402) were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Crystal Violet reagent was acquired from Roche (Mannheim, Germany).
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