Cd4 percpcy5.5 clone

Manufactured by BD

Sourced in United States

CD4-Percpcy5.5 (clone) is a fluorescently labeled monoclonal antibody that binds to the CD4 protein expressed on the surface of T helper cells. It is used in flow cytometry applications to identify and quantify CD4+ T cells in biological samples.

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Lab products found in correlation

Cd3 pe cy7 clone, by BioLegend (1 mentions) Apc mouse igg1, by BD (1 mentions)

2 protocols using cd4 percpcy5.5 clone

Multiparametric Flow Cytometry of Murine Hematopoietic Cells

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Bone marrow and liver MNCs were harvested from the mice. Single-cell suspensions were blocked with an Fc receptor CD16/CD32 (0.5 μL/10⁶ cells) at room temperature. After 10 min, the cells were stained with a cocktail of antibodies. After 30 min of staining at room temperature, the cells were washed with a 1 × phosphate buffer solution.
Monoclonal antibodies for murine lineage antibodies cocktail comprised of Peridinin chlorophyll protein complex (PerCP)-cy5.5 (clone, 51-9006977), CD127-Phycoerythrin (PE)-Cy7 (clone, A7R34), marker of proliferation Ki-67 (Ki67)-PE (clone, SolA), CD4-Percpcy5.5 (clone, RM4-5), and CD8aα-Percpcy5.5 (clone, 53-6.7) were from BD Biosciences (San Diego, CA, USA). Anti-mouse CD45-Percpcy5.5 (clone, 30-F11), CD48-Allophycocyanin (APC)-Cy7 (clone, HM48-1), CD135-PE (clone, A2F10), CD34-Fluorescein isothiocyanate (FITC) (clone, RAM34), CD44-FITC (clone, IM7), CD3-PE-cy7 (clone, 145-2C11), T cell receptor gamma delta (TCRγ/δ)-APC (clone, GL3), Notch1-BV421 (clone, HMN1-12), Notch2-APC (clone, HMN2-35), and stem cell antigen 1 (Sca-1)-APC (clone, D7) were purchased from BioLegend (San Diego, CA, USA). CD117 (c-Kit)-PE-eFluor610 (clone, 2B8), CD25-PE (clone, PC61.5), and TCRβ-PE-eFluor610 (clone, H57-591) were purchased from eBioscience (San Diego, CA, USA).

Qin Y., Fang K., Lu N., Hu Y., Tian Z, & Zhang C. (2019). Interferon gamma inhibits the differentiation of mouse adult liver and bone marrow hematopoietic stem cells by inhibiting the activation of notch signaling. Stem Cell Research & Therapy, 10, 210.

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Flow Cytometry Immunophenotyping Protocol

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Fluorochrome-conjugated monoclonal antibodies (mAbs) were CD4-FITC (Fluoresceine isothiocyanate) clone SK3, CD25-APC (Allophycocyanin) clone 2A3, CD127-PERCP (Peridinin chlorophyll protein)-CY5.5 clone HIL-7R-M21, mouse-IgG1-FITC clone X40, mouse-IgG-PE (Phycoerythrin) clone MOPC-21, mouse-IgG1-APC clone SJ25C1, mouse-IgG1- PERCP-CY5.5 clone X40, CD4- PERCP-CY5.5 clone SK3, CD8-FITC clone 2D1, and CD279(PD-1)-PE clone EH12.1 (BD Biosciences, CA, USA).

Chen Q., Hu W., Xiong H., Ying S., Ruan Y., Wu B, & Lu H. (2019). Changes in plasma EBV-DNA and immune status in patients with nasopharyngeal carcinoma after treatment with intensity-modulated radiotherapy. Diagnostic Pathology, 14, 23.

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