Dexamethasone (dex)

Manufactured by Viatris

Sourced in France

Dexamethasone is a synthetic glucocorticoid medication used as a laboratory reagent. It is a potent anti-inflammatory and immunosuppressant compound. Dexamethasone is commonly used in research applications to modulate cellular processes and gene expression.

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Lab products found in correlation

Il 1β, by Thermo Fisher Scientific (2 mentions) Methylprednisolone, by Viatris (1 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions) Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (1 mentions) Collagenase type 2, by Worthington (1 mentions) Isobuthylmethylxanthine, by Merck Group (1 mentions) Low glucose dmem, by Thermo Fisher Scientific (1 mentions) Oil red o solution, by Bio-Optica (1 mentions) Indomethacin, by Merck Group (1 mentions) Exendin 9 39, by Merck Group (1 mentions) Ldh assay kit, by Abcam (1 mentions) Mouse pge2, by Abcam (1 mentions) Interleukin 6 (il 6), by Abcam (1 mentions) Collagenase d, by Roche (1 mentions) Griess reagent system, by Promega (1 mentions)

4 protocols using dexamethasone (dex)

Macrophage Activation and Modulation

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Monocytes immediately after adherence, or 7-day, 14-day, or 21-day culture macrophages were treated with 0.1 or 2 μg/mL LPS or 0.01, 1, 10 μM dexamethasone (Mylan, Saint Priest, France) for 24 h in the absence of HS unless otherwise stated. Results of time- and concentration-dependent experiments are presented in the supplementary data. Based on these results (S1 and S2 Figs), most of the treatments were done, unless otherwise specified, using 100 ng/mL LPS and 1μM dexamethasone. Methylprednisolone (Mylan 20 mg, Saint Priest France), another synthetic glucocorticosteroid was used on selected experiments performed on 7-day culture macrophages from 4 donors.
Monocytes were alternatively treated after washing with 1X PBS with 100 ng/mL IL1β (Cat No. 200-01B, Peprotech), 100 ng/mL IL6 (Cat No. 200–06, Peprotech) and 1μM dexamethasone (Mylan) for 24 h to compare with the results observed with the HepG2 cell line.
Following treatment, cell culture supernatants were collected and stored at -20°C for cytokine measurement by ELISA and cell lysates were used for RNA isolation, immunoprecipitation or western blot analysis.

Jumeau C., Awad F., Assrawi E., Cobret L., Duquesnoy P., Giurgea I., Valeyre D., Grateau G., Amselem S., Bernaudin J.F, & Karabina S.A. (2019). Expression of SAA1, SAA2 and SAA4 genes in human primary monocytes and monocyte-derived macrophages. PLoS ONE, 14(5), e0217005.

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Isolation and Expansion of Canine Myoblasts

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Myoblasts were isolated from dog BF biopsies. Muscle was minced in small fragments, rinsed with PBS + 2% FBS, and digested with type II collagenase (Worthington) for 1 h at 37°C followed by mechanic trituration using a 18G needle. Digestion product was filtrated with 100 μm and then 40 μm cell strainers. Cells were seeded in a flask with custom MCDB120-modified medium (HyClone) supplemented with 20% FBS (HyClone), 25 μg/mL gentamicin, 10 ng/mL basic fibroblast growth factor (bFGF) (Peprotech), and 10-6 M dexamethasone (Mylan) and cultured in a humidified incubator at 37°C with 20% O₂ and 5% CO₂. This custom made medium allows an enrichment of myoblasts cells through substitution of L-valine by D-valine inducing efficient inhibition of fibroblasts proliferation in benefit of myoblasts proliferation.⁴⁷ (link) Cultured cells were immunophenotyped by the expression of desmin and CD56 as myogenic markers.⁴⁸ (link) Myotube formation was induced by culturing myoblasts in DMEM supplemented with 2% horse serum and 1% penicillin/streptomycin for 3 to 7 days.

Punzón I., Mauduit D., Holvoet B., Thibaud J.L., de Fornel P., Deroose C.M., Blanchard-Gutton N., Vilquin J.T., Sampaolesi M., Barthélémy I, & Blot S. (2020). In Vivo Myoblasts Tracking Using the Sodium Iodide Symporter Gene Expression in Dogs. Molecular Therapy. Methods & Clinical Development, 17, 317-327.

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Adipogenic Differentiation of hMSCs

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hMSCs were incubated in 6-well microplates in DMEM low glucose (Gibco) with 20% FBS, 60 μM indomethacin (Sigma-Aldrich), 0,5 mM isobuthyl methylxanthine (IBMX) (Sigma-Aldrich), 10⁻⁶ M dexamethasone (Mylan) and 1% ATB-ATM solution. Medium was changed every two days. At day 21, cells were stained or frozen at -80°C until RNA isolation. For staining, cells were fixed in 4% paraformaldehyde (PAF) (Electron Microscopy Sciences) and stained in oil red O solution (Bio-Optica).

Boucher H., Vanneaux V., Domet T., Parouchev A, & Larghero J. (2016). Circadian Clock Genes Modulate Human Bone Marrow Mesenchymal Stem Cell Differentiation, Migration and Cell Cycle. PLoS ONE, 11(1), e0146674.

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Investigating GLP-1 Receptor Signaling

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The GLP-1 analog, liraglutide, was purchased from Hybio Pharmaceuticals (Shenzhen, China) and Novo Nordisk (Bagsværd, Denmark). Dexamethasone was purchased from Mylan (Paris, France). Primary rabbit polyclonal immunoglobulin G (IgG) anti-GLP-1R antibody was purchased from Novus Biologicals (NBP1-97308, Centennial, Colo., USA). ImmPRESS^® horseradish peroxidase (HRP) goat anti-rabbit IgG polymer detection kit, BLOXALL^®, VectaFluor^® kit with anti-rabbit IgG Dylight 488 antibody, and VECTASHIELD^® Antifade mounting medium (4′,6-diamidino-2-phenylindole—DAPI) were purchased from Vector Laboratories (Burlingame, CA, USA). The 3,3′-diaminobenzidine (DAB) substrate kit was purchased from Cell Signaling Technology (Leiden, Netherlands). IL-1β was obtained from PeproTech (Neuilly-sur-Seine, France). Collagenase D was purchased from Roche (Basel, Switzerland), Griess reagent system was purchased from Promega (Charbonnière-les-Bains, France), mouse PGE2, IL-6, MMP-3 enzyme linked immunosorbent assay (ELISA), and LDH assay kits were purchased from Abcam (Cambridge, United Kingdom). Mouse ELISA kit for MMP-13 was purchased from Cloud-Clone Corp. (Katy, TX, USA), and glycosaminoglycans (GAG) assay kit was purchased from Chondrex (Redmond, Washington, USA). All culture media and chemicals, including exendin 9–39, were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise stated.

Meurot C., Martin C., Sudre L., Breton J., Bougault C., Rattenbach R., Bismuth K., Jacques C, & Berenbaum F. (2022). Liraglutide, a glucagon-like peptide 1 receptor agonist, exerts analgesic, anti-inflammatory and anti-degradative actions in osteoarthritis. Scientific Reports, 12, 1567.

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