Bis p nitrophenyl phosphate bnpp

Manufactured by Merck Group

Bis (p-nitrophenyl) phosphate (BNPP) is a chemical compound used as a substrate in various analytical and research applications. It is commonly employed in enzyme-based assays, particularly for the detection and quantification of alkaline phosphatase activity. BNPP is a colorless, crystalline solid that hydrolyzes in the presence of alkaline phosphatase, producing a yellow-colored product that can be measured spectrophotometrically.

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Lab products found in correlation

Horseradish peroxidase hrp, by Merck Group (1 mentions) Tetramethylbenzidine, by Merck Group (1 mentions) Thermoscript rt pcr kit, by Thermo Fisher Scientific (1 mentions) Streptavidin mutein matrix, by Roche (1 mentions) Fast track 2.0 kit, by Thermo Fisher Scientific (1 mentions) Freund s adjuvant, by Merck Group (1 mentions) T4 ligase, by New England Biolabs (1 mentions) Goat anti mouse igg alkaline phosphatase, by Merck Group (1 mentions) Bsteii, by New England Biolabs (1 mentions) Mouse anti ha tag antibody, by Fortrea (1 mentions) Ultima flo ap scintillation fluid, by PerkinElmer (1 mentions) Acetonitrile acn, by Merck Group (1 mentions)

Spelling variants of this product

P-nitrophenyl phosphate (490 citations) Bis(p-nitrophenyl) phosphate (4 citations)

2 protocols using bis p nitrophenyl phosphate bnpp

Camelid Antibody Phage Display

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PCT was purchased from Huibiao Biological Technology Co., Ltd. Freund’s adjuvant, horseradish peroxidase (HRP), goat anti-mouse IgG-alkaline phosphatase, Bis (p-nitrophenyl) phosphate (BNPP) and Tetramethylbenzidine (TMB) were purchased from Sigma-Aldrich. Mouse anti-HA tag antibody was obtained from Covance. Fast Track 2.0 Kit and ThermoScript RT-PCR Kit was obtained from InVitrogen. Pst I, Not I, Nco I, BstE II and T4 ligase were obtained from NEB (USA). Streptavidin Mutein Matrix was purchased from Roche. 96-well Maxisorp plates were purchased from Thermo Scientific NUNC. DNA markers were provided by Takara. Protein markers were obtained from Vazyme Biotech Co., Ltd and Thermo Scientific. Polyethylene glycol (PEG) 6000 and Biotin were obtained from Shanghai Sangon Biotech. BeaverNano™ Streptavidin Matrix Coated 96-Well Plates were provided by Beaver. Phagemid vector pMECS, VCSM13 helper phages, Escherichia coli (E. coli) TG1 and WK6 cells, plasmids pBAD17 and pBirA were from Prof. Serge Muyldermans’s lab (Laboratory of Cellular and Molecular Immunology, VUB-Vrije Universiteit, Brussel, Belgium). Bactrian camels were provided by “Joint Center for Nanobody Research & Development between SEU and Egens Bio”. Affinity analysis by surface plasmon resonance imaging (SPRi) binding assay was performed on PlexArray ® HT system (Plexera LLC).

Yan J., Wang P., Zhu M., Li G., Romão E., Xiong S, & Wan Y. (2015). Characterization and applications of Nanobodies against human procalcitonin selected from a novel naïve Nanobody phage display library. Journal of Nanobiotechnology, 13, 33.

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Radiolabelled Carboxylate Ester Hydrolysis

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[¹⁴C]-diethylene triamine pentaacetic acid pentaethyl ester ([¹⁴C]-C2E5; 50 mCi/mmol) was purchased from American Radiolabeled Chemicals, Inc. (St. Louis, MO). Sprague-Dawley rat intestinal S9 fractions, isolated from a single male were purchased from Celsis In Vitro Technologies (Baltimore, MD). Pooled, mixed gender human (intestinal, hepatic), single male Beagle dog (intestinal, hepatic), and single male Sprague-Dawley (hepatic) S9 fractions were purchased from Xenotech, LLC (Lenexa, KS). All S9 fractions were prepared in the absence of phenylmethanesulfonylfluoride. Human CES1 and CES2 supersomes were obtained from BD Biosciences (Woburn, MA). Human, Beagle dog, and Sprague-Dawley rat plasma was purchased from Lampire Biological Laboratories, Inc. (Pipersville, PA). Ultima-Flo AP scintillation fluid was obtained from PerkinElmer Life and Analytical Sciences (Waltham, MA). Acetonitrile (ACN) and bis-(p-nitrophenyl) phosphate (BNPP) were purchased from Sigma Aldrich (St. Louis, MO).

Fu J., Pacyniak E., Leed M.G., Sadgrove M.P., Marson L, & Jay M. (2016). Interspecies Differences in the Metabolism of a Multi-Ester Prodrug by Carboxylesterases. Journal of pharmaceutical sciences, 105(2), 989-995.

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