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4 protocols using myricitrin

1

Isolation and Characterization of Phenolic Compounds

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Gallic (3), ellagic (38), protocatechuic (45), neochlorogenic (48), caffeic (49), chlorogenic (50), syringic acids (53), myricitrin (59), isoquercitrin (60), hyperoside (64), kaempferol 3-O-glucoside (67), quercitrin (68), isorhamnetin 3-O-glucoside (72), quercetin (77), myricetin (78), kaempferol (80), (+)-catechin (82), and prunin (87) were obtained from Extrasynthese (Genay, France). All reagents and solvents were of analytical grade.
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2

Comprehensive Phytochemical Profiling Protocol

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The solvents used for extraction (methanol, acetonitrile and formic acid) were purchased from Sigma-Aldrich Chemical Company (St Louis, MO, USA). A Milli-Q purification system (Millipore, Bedford, MA, USA) was used to prepare high-performance liquid chromatography (HPLC) grade water. Standards of gallic acid, caffeic acid, p-coumaric acid, ellagic acid, ferulic acid, sinapic acid, quinic acid, syringic acid, chlorogenic acid, phloridzin, kaempferol, luteolin, quercetin, isorhamnetin, myricetin, apigenin, epicatechin and catechin were purchased from Sigma-Aldrich. Standards of quercetin rhamnoside, isoquercetin, rutin, robinin, isorhamnetin rutinoside, neohesperidin, quercetin galactoside, myricitrin, myricetin galactoside, epigallocatechin, epigallocatechin gallate, procyanidin B1, procyanidin B2, cyanidin-3-O-glucoside, cyanidin-3-O-arabinoside, cyanin, delphinidin-3-O-glucoside, malvidin-3-O-glucoside, and pelargonidin-3-O-glucoside chloride were purchased from Extrasynthese (Genay, France).
The reference standard mixture of 37 FAME (FAME mix 37) was acquired from Supelco (Sigma-Aldrich, Bellefonte, PA, USA); other reference standards were purchased from Matreya Inc. (Pleasant Gap, PA, USA): PUFA-2, a nonconjugated 18:2 isomer mixture of individual PUFA, eicosapentaenoic acid, (EPA), docosahexaenoic acid (DHA), arachidonic acid (ARA), C18:3 cis-6,9,12, and C18:3 cis-9,12,15.
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3

HPLC Analysis of Phenolic Compounds

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Formic acid, ethanol, HPLC grade water, and acetonitrile were supplied by Merk Life Science S.r.l. (Milano, Italy). Chlorogenic acid was purchased from Phytolab (Aprilia, Italy). Gallic acid, ferulic acid, 4-coumaric acid, caffeic acid, procyanidin B1, procyanidin B2, quercitrin, myricitrin, and quercetin were purchased from Extrasynthese (Genay, France) and used as HPLC reference standards.
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4

Quantitative Analysis of Polyphenols

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Technical
grade acetone for
extraction was purchased from VWR (Haasrode, Belgium). Formic acid
(HCOOH) and LC-MS Chromasolv acetonitrile for the UHPLC-ESI-QqQ-MS
were obtained from Sigma-Aldrich (Seelze, Germany), and catechin for
the catechin stock solution was acquired from Sigma-Aldrich (St. Louis,
MO, USA). Kaempferol-3-O-rutinoside, myricitrin,
caffeoylquinic acid, arbutin, and quercetin-O-rutinoside
for the calibration curves, as well as kaempferol-7-O-glucoside, kaempferol-7-O-neohesperoside, kaempferol-3-O-glucoside, hyperoside, and quercetin glucopyranoside for
the stock solutions were obtained from Extrasynthese (Genay, France).
Digalloylglucose (98% purity, as determined by UPLC-DAD) for the calibration
of galloylated compounds was purified by J.-P. Salminen (University
of Turku, Turku, Finland) from a Betula pubescens leaf extract by a combination of Sephadex LH-20 gel chromatography
and semipreparative HPLC. Water was purified with a Millipore Synergy
water purification system from Merck KGaA (Darmstadt, Germany). Sephadex
LH-20 was obtained from GE Healthcare (Uppsala, Sweden).
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