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2 protocols using dspe peg

1

Nano Drug Delivery System Development

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Hydrogenated soybean phosphatidylcholine (HSPC) and N-(carbonyl-methoxypolyethyleneglycol2000)-1,2-distearoyl-snglycero-3-phosphoethanolamine (DSPE-PEG) were purchased from NOF Corporation (Tokyo, Japan). Cholesterol was obtained from Tokyo Chemical Industry Co., Ltd (Tokyo, Japan). 1,1′-Dioctadecyltetramethyl indotricarbocyanine iodide (XenoLight DiR) was supplied by Caliper Life Sciences (Hopkinton, MA, USA). l-Arginine hydrochloride, doxorubicin, lipopolysaccharides (LPS) from Escherichia coli were purchased from Sigma Chemical Co. (St. Louis, MO, USA). l-Arginine deficient SILAC Dulbecco's modified Eagle's medium (DMEM) was obtained from Thermo Scientific (Waltham, MA, USA).
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2

Lipid Nanoparticle Synthesis and Characterization

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1,2-Distearoryl-sn‑glycero-3-phosphoethanolamine-N-(methoxy[polyethyleneglycol-2000]) ammonium salt (DSPE-PEG) was purchased from NOF Corporation (Tokyo, Japan). DSPE-PEG-mannose was obtained from Xi'an Ruixi Biological Technology Co., Ltd (Shanxi, China). Dioleoylphosphatydic acid (DOPA) and 1,2-dioleoyl-3-trimethylammonium-propane chloride salt (DOTAP) were sourced from Avanti Polar Lipids (Alabaster, AL, USA). Cholesterol, cyclohexane, and IGEPAL-CO-520 were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals were purchased from Sigma-Aldrich if not specifically mentioned. Nucleotides (ATP and guanosine triphosphate [GTP]) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Modified nucleotides (5-methylcytidine-5′-triphosphate and pseudouridine-5′-triphosphate) were obtained from Trilink Biotechnologies (San Diego, CA, USA). 3′-O-Me-m7G (5′) ppp (5′) G RNA cap structure analog was purchased from NEB (Ipswich, MA, USA).
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