Cd8 bv711 clone rpa t8

Manufactured by BioLegend

CD8-BV711 (clone RPA-T8) is a fluorochrome-conjugated monoclonal antibody that binds to the CD8 antigen. CD8 is a cell surface glycoprotein expressed on a subset of T cells and Natural Killer cells.

Automatically generated - may contain errors

Lab products found in correlation

Cd28 ecd clone cd28 2, by Beckman Coulter (3 mentions) Ccr7 clone 150503, by R&D Systems (2 mentions) Cd3 clone sp34 2 pe cf594, by BioLegend (2 mentions) Cd20 clone 2h7 alexa700, by BioLegend (2 mentions) Pd 1 clone eh12.2h7 bv605, by Beckman Coulter (2 mentions) Nkg2a clone z199 pe cy7, by Thermo Fisher Scientific (2 mentions) Near infrared live dead discriminator, by Thermo Fisher Scientific (2 mentions) Lsr 2, by BD (2 mentions) Fluorescein isothiocyanate (fitc), by R&D Systems (2 mentions) Facsdiva software package, by BD (2 mentions) Pd 1 bv421 clone eh12.2h7, by BioLegend (2 mentions) Lsrii flow cytometer, by BD (2 mentions) Cd4 bv650 clone okt4, by BioLegend (2 mentions) Cd3 apc cy7 clone sp34 2, by BD (1 mentions) Cd95 pe cy5 clone dx2, by BD (1 mentions) Hla dr percp cy5.5 clone g46 6, by BD (1 mentions) Cd45ra fitc clone l48, by BD (1 mentions) Streptavidin pe, by BioLegend (1 mentions) Ccr7 pe cy7 clone 3d12, by BD (1 mentions) Brilliant stain buffer, by BD (1 mentions)

Spelling variants of this product

RPA-T8 (46 citations) CD8 (clone RPA-T8; (17 citations) CD8-BV711 (15 citations) Clone RPA-T8 (13 citations)

6 protocols using cd8 bv711 clone rpa t8

Multiparameter Flow Cytometry Phenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

We pre-incubated 1 × 10⁶–3 × 10⁶ cells with APC-conjugated VY9, or NP8 tetramer at 37°C, 5% CO₂ for one hour. (The tetramers were produced by the NIH Tetramer Core Facility at Emory University, Atlanta, GA.) Next, we added the antibodies recognizing the surface phenotypic markers. We used antibodies from BD Biosciences: CCR7 (clone 150503, R&D Systems) FITC, CD3 (clone SP34-2) PE-CF594, CD8 (clone RPA-T8) BV711, CD20 (clone 2H7) Alexa700, CD28 (clone CD28.2,) PE, CD45 (clone D058-1283) BV786; from BioLegend: PD-1 (clone EH12.2H7) BV605; from Beckman Coulter: NKG2a (clone Z199) PE-Cy7, and Near-Infrared Live/Dead Discriminator from Life Technologies. We incubated the cells for 15 min at room temperature, removed any unbound reagents with two washing steps then fixed them with 2% PFA. We acquired the data on a special-order BD LSR II (BD Biosciences, San Jose, CA) equipped with a 50 mW 405 nm violet, a 100 mW 488 nm blue, and a 50 mW 640 nm red laser, 16 detectors, and FACSDiva software version 8.0.1. We collected up to 250,000 events in the lymphocyte gate defined by the forward and side scatter parameters. We analyzed the data using FlowJo version 10.4.2.

Pomplun N.L., Vosler L., Weisgrau K.L., Furlott J., Weiler A.M., Abdelaal H.M., Evans D.T., Watkins D.I., Matano T., Skinner P.J., Friedrich T.C, & Rakasz E.G. (2020). Immunophenotyping of Rhesus CMV‐Specific CD8 T‐Cell Populations. Cytometry, 99(3), 278-288.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry of T-cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

We pre‐incubated 1 × 10⁶–3 × 10⁶ cells with APC‐conjugated VY9, or NP8 tetramer at 37°C, 5% CO₂ for one hour. (The tetramers were produced by the NIH Tetramer Core Facility at Emory University, Atlanta, GA.) Next, we added the antibodies recognizing the surface phenotypic markers. We used antibodies from BD Biosciences: CCR7 (clone 150503, R&D Systems) FITC, CD3 (clone SP34‐2) PE‐CF594, CD8 (clone RPA‐T8) BV711, CD20 (clone 2H7) Alexa700, CD28 (clone CD28.2,) PE, CD45 (clone D058‐1283) BV786; from BioLegend: PD‐1 (clone EH12.2H7) BV605; from Beckman Coulter: NKG2a (clone Z199) PE‐Cy7, and Near‐Infrared Live/Dead Discriminator from Life Technologies. We incubated the cells for 15 min at room temperature, removed any unbound reagents with two washing steps then fixed them with 2% PFA. We acquired the data on a special‐order BD LSR II (BD Biosciences, San Jose, CA) equipped with a 50 mW 405 nm violet, a 100 mW 488 nm blue, and a 50 mW 640 nm red laser, 16 detectors, and FACSDiva software version 8.0.1. We collected up to 250,000 events in the lymphocyte gate defined by the forward and side scatter parameters. We analyzed the data using FlowJo version 10.4.2.

+ Open protocol

+ Expand

Multicolor Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Multicolor flow cytometric analysis was performed on whole blood or a cell suspension using predetermined optimal concentrations of the following fluorescently conjugated monoclonal antibodies (MAbs): CD3-APC-Cy7 (clone SP34-2), CD95-PE-Cy5 (clone DX2), K_i-67-AF700 (clone B56), HLA-DR-PerCP-Cy5.5 (clone G46-6), CCR7-FITC (clone 150503), CCR5-APC (clone 3A9), and CD45RA-PECy7 (clone L48) from BD Biosciences; CD8-BV711 (clone RPA-T8), CD4-BV650 (clone OKT4), and PD-1-BV421 (clone EH12.2H7) from BioLegend, and CD28-ECD (clone CD28-2) from Beckman Coulter. Flow cytometric acquisition and analysis of samples were performed on at least 100,000 events on an LSR II flow cytometer driven by the FACSDiva software package (BD Biosciences). Analyses of the acquired data were performed using FlowJo version 10.0.4 software (TreeStar).

Mavigner M., Zanoni M., Tharp G.K., Habib J., Mattingly C.R., Lichterfeld M., Nega M.T., Vanderford T.H., Bosinger S.E, & Chahroudi A. (2019). Pharmacological Modulation of the Wnt/β-Catenin Pathway Inhibits Proliferation and Promotes Differentiation of Long-Lived Memory CD4+ T Cells in Antiretroviral Therapy-Suppressed Simian Immunodeficiency Virus-Infected Macaques. Journal of Virology, 94(1), e01094-19.

+ Open protocol

+ Expand

Multicolor Flow Cytometry of PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Multicolor flow cytometric analysis was performed on whole blood or frozen PBMCs using predetermined optimal concentrations of the following fluorescently conjugated mAbs: CD3-PacBlue or -APC-Cy7 (clone SP34-2), CD95-PE-Cy5 (clone DX2), Ki-67-AF700 (clone B56), HLA-DR-PerCP-Cy5.5 (clone G46-6), CCR7-PE-Cy7 (clone 3D12), CCR5-PE or -APC (clone 3A9), CD45RA-FITC (clone L48), Biotin-CD122 (clone Mik-β3) from BD Biosciences; CD8-BV711 (clone RPA-T8), CD4-APC-Cy7 or -BV650 (clone OKT4), Streptavidin-PE from Biolegend, and CD28-ECD (clone CD28-2) from Beckman-Coulter. Flow cytometric acquisition and analysis of samples was performed on at least 100,000 events on an LSRII flow cytometer driven by the FACSDiva software package (BD Biosciences). Analyses of the acquired data were performed using FlowJo Version 10.0.4 software (TreeStar).

Mavigner M., Watkins B., Lawson B., Lee S.T., Chahroudi A., Kean L, & Silvestri G. (2014). Persistence of Virus Reservoirs in ART-Treated SHIV-Infected Rhesus Macaques after Autologous Hematopoietic Stem Cell Transplant. PLoS Pathogens, 10(9), e1004406.

+ Open protocol

+ Expand

Comprehensive Flow Cytometry Immune Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flow cytometry staining was performed as previously described (57 (link)–59 (link)). In brief, 1 × 10⁶ PBMCs were stained with Zombie (BioLegend) fixable viability dye for 30 min at 4°C, then labeled with antibodies to surface markers in Brilliant Stain buffer (BD Biosciences) for 30 min at 4°C. Subsequently samples were stained with 25 μg/mL cholera toxin B subunit FITC conjugate (CTB-FITC) (Sigma-Aldrich), fixed for 1 h in 2% paraformaldehyde, and stained for 2 h with 50 μg/mL filipin complex from Streptomyces filipinensis (Sigma-Aldrich) before reading the samples on a BD LSRFortessa X-20 cytometer using BD FACSDiva software. Compensation was performed using anti-mouse IgGκ/negative control compensation particles set (BD Biosciences) or OneComp eBeads (ThermoFisher Scientific), with the exception of viability dyes and filipin which were performed with single stained and unstained cells. Data was analyzed using FlowJo (Tree Star).
Antibodies for surface markers: CD45RA-BUV737 (clone HI100, BD Biosciences, 584442) CD27–APC (clone M-T271, BioLegend, 356409), CD4-AF700 (clone OKT4, eBioscience, 56-0048-82), CCR7-BV421 (clone G043H7, BioLegend, 353207), CD69-BV510 (clone FN50, BioLegend, 310936), CD8-BV711 (clone RPA-T8, BioLegend, 301044), CD3-BV785 (clone OKT3, BioLegend, 317330), CD25-PE (clone M-A251, BioLegend, 356104), CD127-PE-Cy7 (clone A019D5, BioLegend, 351320).

Waddington K.E., Papadaki A., Coelewij L., Adriani M., Nytrova P., Kubala Havrdova E., Fogdell-Hahn A., Farrell R., Dönnes P., Pineda-Torra I, & Jury E.C. (2020). Using Serum Metabolomics to Predict Development of Anti-drug Antibodies in Multiple Sclerosis Patients Treated With IFNβ. Frontiers in Immunology, 11, 1527.

+ Open protocol

+ Expand

Multicolor Flow Cytometric Profiling of T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Multicolor flow cytometric analysis was performed on whole blood (WB) or cell suspensions using predetermined optimal concentrations of the following fluorescently conjugated monoclonal antibodies (MAbs). For WB T cell analysis the following MAbs were used: CD3-allophycocyanin (APC)-Cy7 (clone SP34-2), CD95-phycoerythrin (PE)-Cy5 (clone DX2), Ki67-AF700 (clone B56), HLA-DR-peridinin chlorophyll protein (PerCP)-Cy5.5 (clone G46-6), CCR7-fluorescein isothiocyanate (FITC) (clone 150503), CCR5-APC (clone 3A9), CD62L (clone SK11), and CD45-RA-PE-Cy7 (clone L45) from BD Biosciences; CD8-BV711 (clone RPA-T8), CD4-BV650 (clone OKT4), and PD-1-BV421 (clone EH12.2H7) from BioLegend; and CD28-ECD (clone CD28-2) from Beckman-Coulter. Flow cytometric acquisition and analysis of samples were performed on at least 100,000 events on an AURORA flow cytometer driven by the SpectroFlo software package (Cytek). Analyses of the acquired data were performed using FlowJo version 10.0.4 software (TreeStar).

Bricker K.M., Obregon-Perko V., Williams B., Oliver D., Uddin F., Neja M., Hopkins L., Dashti A., Jean S., Wood J.S., Ehnert S., Liang S., Vanderford T., Tharp G.K., Bosinger S.E., Schauer A.P., Mavigner M., Cottrell M.L., Margolis D., Dunham R.M, & Chahroudi A. (2022). Altered Response Pattern following AZD5582 Treatment of SIV-Infected, ART-Suppressed Rhesus Macaque Infants. Journal of Virology, 96(7), e01699-21.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!