Concentrations of IL-6 in the bronchoalveolar lavage fluid (BALF), serum and lung tissues were measured with murine cytokine-specific ELISA kits (R&D Systems, Minneapolis, MN, USA) according to the manufacturer's instructions. Protein contents in the BALF were assayed by bicinchoninic acid (BCA) kit (Beyotime, China). IL-6 levels in the BALF and lung tissues were normalized by corresponding protein concentrations.
Murine cytokine specific elisa kits
Manufactured by R&D Systems
Sourced in United States, United Kingdom
The Murine cytokine-specific ELISA kits are laboratory equipment designed to detect and quantify specific cytokines in murine (mouse) samples. These kits utilize the enzyme-linked immunosorbent assay (ELISA) technique to provide a quantitative measurement of the target cytokine. The core function of these kits is to enable researchers to analyze the presence and concentration of selected cytokines in their murine-based studies.
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Lab products found in correlation
2 protocols using murine cytokine specific elisa kits
1
Measuring IL-6 in Lung Samples
2
Measuring Lung Cytokines and Nox4 Levels
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Concentrations of TNF-α and IL-1β in the lung tissues were measured with murine cytokine-specific ELISA kits (R&D Systems, Abingdon, UK). In brief, the lung homogenate was centrifuged at 3000 rpm for 20 min at 4°C, and the supernatant was analyzed using ELISA kits according to the manufacturer's instructions.
The level of NADPH oxidase 4 (Nox4) in HPAECs was measured. The cells were treated as described above, and the ELISA was conducted according to the manufacturer's protocols (Yifeixue Biotechnology, Nanjing, China).
The level of NADPH oxidase 4 (Nox4) in HPAECs was measured. The cells were treated as described above, and the ELISA was conducted according to the manufacturer's protocols (Yifeixue Biotechnology, Nanjing, China).
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