Acetyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody (1:1000, Beyotime, AF5611) was used to detect Ac-H3 protein. HDAC11 (1:1000, Gene Tex, GTX1164031) was purchased from Gene Tex. Enterovirus 71 antibody (1:1000, Abcam, ab36367) and Enterovirus 71 antibody (1:1000, GeneTex, GTX132339) were used to detect VP1 protein, respectively. Antibody against GAPDH was obtained from Protein Tech Group (Cat. NO. 10,494–1-AP) (Wuhan, China). FT895 (HY-112,285) was obtained from MCE (Med Chem Express) and dissolved in DMSO. DRAQ5 is a far-red DNA stain for use in live or fixed cells and is purchased from ebioscience (San Diego, USA). The plasmids expressing human HDAC11 (pECMV-3xflag-HDAC11, p4170) were purchased from miaolingbio (Wuhan, Hubei, China). pLenti-CMV-HDAC11-GFP-puro lentiviral plasmid (Lenti-HDAC11-GFP, Cat#: PPLBC009676) and pPLK/GFP+Puro-HDAC11 shRNA (Cat#: PPL79895) were obtained from Public Protein/Plasmid Library (PPL). The lentiviral packaging plasmid pMD2.G (Cat#: 12,259) and psPAX2 (Cat#: 12,260) were obtained from AddGene.
Gtx132339
Manufactured by GeneTex
Sourced in United States
GTX132339 is a laboratory centrifuge designed for the separation of biological samples, such as cells, organelles, and macromolecules. It is capable of providing high-speed centrifugation to achieve efficient separation and purification of various samples.
Automatically generated - may contain errors
Lab products found in correlation
Draq5, by Thermo Fisher Scientific (1 mentions)
Pspax2, by Addgene (1 mentions)
Pmd2 g, by Addgene (1 mentions)
Ab179484, by Abcam (1 mentions)
Polyvinylidene difluoride membrane, by Merck Group (1 mentions)
Surepage bis tris gel, by GenScript (1 mentions)
Gtx132346, by GeneTex (1 mentions)
Pierce ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions)
Quantity one software, by Bio-Rad (1 mentions)
Ab9485, by Abcam (1 mentions)
2 protocols using gtx132339
1
Enterovirus 71 Protein Detection
2
Western Blotting of Stress Proteins
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Cells were treated as indicated and Western blotted as previously described (Liu et al., 2015 (link)). Briefly, the cells were lysed and assessed using 4–20% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SurePAGE, Bis-Tris gels, Genscript, Nanjing, China). Proteins were transferred to polyvinylidene difluoride membranes (Millipore, Bedford, MA, United States) and probed with primary antibodies against HSPA6 (sc-374589, Santa Cruz Biotechnology), HSPA1A (PA534772, Invitrogen), HSPA8 (PA5-27337, Invitrogen), HSPB1 (PA1017, Invitrogen), EV71 VP1 (GTX132339, GeneTex), CVA6 VP1 (GTX132346, GeneTex), GAPDH (ab9485, Abcam) or α-Tubulin (ab179484, Abcam). Protein bands were visualized with Pierce™ ECL Western blotting substrate (Thermo Scientific, Rockford, IL, United States). Protein levels were determined by scanning the bands’ intensities and analyzed using Quantity One software (Bio-Rad, Hercules, CA, United States).
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