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2 protocols using fas mouse monoclonal antibody m01

1

Quantitative Analysis of Immune Cell Subsets

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Primary antibodies used: APC/Cy7 anti-human CD3 (Clone SK7, BioLegend), FITC anti-human CD4 (Clone RPA-T4, BioLegend), anti-human CD8a PE-Cy5 (Clone RPA-T8, eBioscience), PE-Cy™7 Mouse Anti-Human CD45RA (Clone HI100, BD Biosciences), APC anti-human CD45RA (Clone HI100, BioLegend), APC Mouse Anti-Human CD45RO (Clone UCHL1, BD Biosciences), Brilliant Violet 711™ anti-human CD45 (HI30, BioLegend), cortactin antibody (PA5-29799) (ThermoFisher Scientific), FAS mouse monoclonal antibody (M01) (Clone 3F2-1F3, Abnova), Acetyl-CoA Carboxylase (C83B10) Rabbit mAb (Cell Signaling Technology), SCD1 (C12H5) Rabbit mAb (Cell Signaling Technology), RANKL/CD254 Antibody (12A668) (ThermoFisher Scientific), Monoclonal Mouse Anti-Human CD3 (Clone F7.2.38, Dako), Polyclonal Rabbit Anti-Human CD3 (#A045201-2, Dako), β-Actin (8H10D10) Mouse mAb (#3700, Cell Signaling), Anti-rabbit IgG, HRP-linked Antibody (#7074, Cell Signaling), WesternSure HRP Goat anti-Mouse IgG (H+L) (LI-COR), Anti-Interferon gamma antibody (ab25101, Abcam), Anti-PPAR gamma antibody (ab178860, Abcam), PPARγ (81B8) Rabbit mAb (Cell Signaling), Monoclonal anti-flag (HRP) antibody (A8592-.2MG, Sigma-Aldrich).
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2

Quantitative Analysis of Immune Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies used: APC/Cy7 anti-human CD3 (Clone SK7, BioLegend), FITC anti-human CD4 (Clone RPA-T4, BioLegend), anti-human CD8a PE-Cy5 (Clone RPA-T8, eBioscience), PE-Cy™7 Mouse Anti-Human CD45RA (Clone HI100, BD Biosciences), APC anti-human CD45RA (Clone HI100, BioLegend), APC Mouse Anti-Human CD45RO (Clone UCHL1, BD Biosciences), Brilliant Violet 711™ anti-human CD45 (HI30, BioLegend), cortactin antibody (PA5-29799) (ThermoFisher Scientific), FAS mouse monoclonal antibody (M01) (Clone 3F2-1F3, Abnova), Acetyl-CoA Carboxylase (C83B10) Rabbit mAb (Cell Signaling Technology), SCD1 (C12H5) Rabbit mAb (Cell Signaling Technology), RANKL/CD254 Antibody (12A668) (ThermoFisher Scientific), Monoclonal Mouse Anti-Human CD3 (Clone F7.2.38, Dako), Polyclonal Rabbit Anti-Human CD3 (#A045201-2, Dako), β-Actin (8H10D10) Mouse mAb (#3700, Cell Signaling), Anti-rabbit IgG, HRP-linked Antibody (#7074, Cell Signaling), WesternSure HRP Goat anti-Mouse IgG (H+L) (LI-COR), Anti-Interferon gamma antibody (ab25101, Abcam), Anti-PPAR gamma antibody (ab178860, Abcam), PPARγ (81B8) Rabbit mAb (Cell Signaling), Monoclonal anti-flag (HRP) antibody (A8592-.2MG, Sigma-Aldrich).
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