Ca2 mg2 atpase assay kit

Treated mites were washed three times with normal saline (0.9% w/v of NaCl solution). Samples were homogenized in 900 μl of normal saline and centrifuged at 2500 r/min (4 °C) for 10 min. The supernatant was used to determine the water-soluble protein concentration, using a total protein quantification assay kit (A045-3, Nanjing Jiancheng Bioengineering Institute, China). Briefly, the proteins reduced cupric ion to cuprous ion, which was further adducted with bicinchoninic acid (BCA). The product has a maximum absorbance at 562 nm. The protein content was calculated based on the absorbance of the solution.
The enzyme bioactivities of GST, SOD and Ca²⁺-Mg²⁺-ATPase were evaluated using a GST assay kit (catalog no. A004, Nanjing Jiancheng Bioengineering Institute, China), an SOD assay kit (catalog no. A001-3, Nanjing Jiancheng Bioengineering Institute, China) and a Ca²⁺-Mg²⁺-ATPase assay kit (catalog no. A070-3, Nanjing Jiancheng Bioengineering Institute, China) according to the manufacturer’s instructions.

The serum lactate levels, FFA concentrations, sodium-potassium-ATPase (Na+-K+-ATPase) activities, calcium-magnesium-ATPase (Ca2+-Mg2+-ATPase) activities, and the erythrocyte membrane ATPase activities were detected by colorimetry. Lactic acid assay kit, Non-esterified free fatty acids assay kit, Na+-K+-ATPase assay kit, and Ca2+-Mg2+-ATPase assay kit were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The experimental steps were in strict accordance with the manufacturer's instructions.