Curcumin was supplied from LKT Laboratories (St. Paul, MN, USA). TetrahydroCurcumin was prepared by conventional catalytic hydrogenation of Curcumin in the presence of palladium on carbon. DSS (molecular weight, 36,000–50,000) was obtained from ICN Biochemicals, Inc. (Aurora, OH, USA) and MP Biomedical Inc. (Solon, OH, USA). Rabbit polyclonal COX-2 antibody was purchased from Cayman Chemical Co. (Ann Arbor, MI, USA), and iNOS antibody from BD Bioscience (Franklin Lakes, NJ, USA). Primary antibody for β-catenin was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-rabbit and anti-mouse horseradish peroxidase-conjugated secondary antibodies were products of Zymed Laboratories (San Francisco, CA, USA). An oligonucleotide probe containing the NF-κB sequence in the mouse COX-2 promoter region was obtained from Bionics (Seoul, Korea). An oligonucleotide probes harboring the STAT3 consensus sequence was obtained from Santa Cruz Biotechnology.
Primary antibody for β catenin
Manufactured by Santa Cruz Biotechnology
Sourced in United States
This primary antibody for β-catenin is a specific and sensitive tool for the detection of this key signaling protein. β-catenin is a critical component of the Wnt signaling pathway and plays a role in cell-cell adhesion. This antibody can be used in various applications such as Western blotting, immunohistochemistry, and immunofluorescence to study the expression and localization of β-catenin.
Automatically generated - may contain errors
Lab products found in correlation
Inos antibody, by BD (1 mentions)
Rabbit polyclonal cox 2 antibody, by Cayman Chemical (1 mentions)
Anti rabbit and anti mouse horseradish peroxidase conjugated secondary antibodies, by Zymo Research (1 mentions)
Curcumin, by LKT Laboratories (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
N cadherin, by Santa Cruz Biotechnology (1 mentions)
2 protocols using primary antibody for β catenin
1
Curcumin and Tetrahydrocurcumin Regulation of Inflammatory Pathways
2
Immunofluorescence Imaging of EMT Markers
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A375 cells (2.5 x 10 5 cells/well) were seeded on glass cover-slips. After 24 hours cells were fixed with formalin for 10 min and permeabilized with PBS 0.25% Triton x100 for 10 min, incubated with 1% BSA for 30 min and stained overnight at 4 °C with primary antibody for β-catenin, Vimentin and N-cadherin (Santa Cruz). Slips were washed three times with PBS and then incubated 1 hour at room temperature with Alexa Fluor 568 or 488 secondary antibodies (ThermoFisher Scientific, Waltham, MA, USA). Nuclei were stained with 1 μg/ml DAPI (D1306 Invitrogen, USA) for 1 min after removal of secondary antibody. Microscopy imaging was performed on Olympus IX71/X51 (Olympus Life science) inverted microscope using a 60X objective [43] (link).
The Corrected Total Cell Fluorescence (CTCF) was measured in at least 15 view fields at 600X magnification. The images were taken at the same exposition time and light intensity. Then, the data were evaluated using the ImageJ software measuring total cell area and a region next to
The Corrected Total Cell Fluorescence (CTCF) was measured in at least 15 view fields at 600X magnification. The images were taken at the same exposition time and light intensity. Then, the data were evaluated using the ImageJ software measuring total cell area and a region next to
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