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2 protocols using rabbit anti u2af65 ab37530

1

Antibody Characterization for DNA Damage Response

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Antibodies were as follows: rabbit anti-FANCD2 (NB100-182; Novus) at 1:10,000; rabbit anti-FANCI (A301-254A; Bethyl) at 1:1,000; rabbit anti-SF3B1 (A300-996A; Bethyl) at 1:2,000; rabbit anti-SF3B1 (Eurogentec) at 1:1,000; rabbit anti-SF3A3 (A302-507A; Bethyl) at 1:2,000; rabbit anti-FLAG (F7425; Sigma-Aldrich) at 1:1,000; rabbit anti-DHX15 (A300-389A; Bethyl) at 1:5,000, mouse anti-SC35 (556363; BD Pharmingen) at 1:500; rabbit anti-U2AF65 (ab37530; Abcam) at 1:250; rabbit anti-pSer345(Chk1) (2348L, rabbit; Cell Signaling Technology) at 1:1,000; mouse anti-Chk1 (Sc-8408; Santa Cruz Biotechnology) at 1:1,000; mouse anti-tubulin (T5168; Sigma-Aldrich) at 1:10,000; anti-rabbit HRP-linked (7074; Cell Signaling Technology) at 1:2,500; anti-mouse HRP-linked (7076; Cell Signaling Technology) at 1:2,500; rabbit anti-Nbs1 (NB100-143; Novus Biologicals) at 1:1,000; mouse anti-PCNA (P8825; Sigma-Aldrich) at 1:3,000; rabbit anti-TopBP1 (A300-111A; Bethyl) at 1:1,000; rabbit anti-RNA-PolII (N-20) (sc-899; Santa Cruz Biotechnology) at 1:1,000; rabbit anti-TFIP11 (A302-548A; Bethyl) at 1:1,000; rabbit anti-SPF45 (A302-548A; Bethyl) at 1:1,000; rabbit anti-FANCA (A301-980A; Bethyl) at 1:2,000; and rabbit anti-PRP8 (GTX108046; GeneTex) at 1:1,000.
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2

Comprehensive Antibody Panel for Cellular Analysis

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Goat anti-TIAR (C-18; sc-1749), goat anti-TIA1 (sc-1671), goat anti-eIF3B (eIF3eta; sc-16377), rabbit anti-p38 MAPK (sc-535), and mouse anti-G3BP (sc-81940) are from Santa Cruz; mouse anti-phospho-RPOL2A (Ser2; H5; ab24758), mouse anti-U1A (ab55751), rat anti-U1C (ab122901), rabbit anti-H3 (ab1791), rabbit anti-PRP19 (ab27692), rabbit anti-PRPF3 (ab187535), and rabbit anti-U2AF65 (ab37530) are from Abcam; rabbit anti-phospho-H3 (Ser10; #9701), rabbit anti-phospho-p38 MAPK (Thr180/Tyr182; #9211), and rabbit anti-phospho-SF3B1 (Thr313; #25009) are from Cell Signaling Technology; mouse anti-U1-70K (05-1588) and mouse anti-puromycin (MABE343) are from Millipore; mouse anti-SC35 (S4045), rabbit anti-SON (HPA031755), and rabbit anti-CASC3 (HPA050262) are from Sigma-Aldrich; and rabbit anti-Calnexin polyclonal antibody (ADI-SPA-860-F) is from Enzo.
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