Qubit 1x dsdna hs assay kits

Manufactured by Agilent Technologies

Sourced in United States

The Qubit 1X dsDNA HS Assay kits are a highly sensitive fluorescence-based method for quantifying double-stranded DNA (dsDNA) in solution. The assay uses a proprietary fluorescent dye that binds selectively to dsDNA, providing a linear quantification range from 0.2 to 100 ng/mL.

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Lab products found in correlation

Qubit 4 fluorometer, by Agilent Technologies (2 mentions) Agilent dna 1000 chip, by Agilent Technologies (2 mentions) Agilent bioanalyzer 2100 system, by Agilent Technologies (2 mentions) Kapa hifi hotstart readymix, by Illumina (1 mentions) Nextera xt index kit, by Illumina (1 mentions) Agilent hs dna chip, by Agilent Technologies (1 mentions) Ampure xp bead, by Beckman Coulter (1 mentions)

Spelling variants of this product

DsDNA HS assay (7 citations) Qubit dsDNA HS Kit (5 citations) DsDNA HS (5 citations) Qubit dsDNA HS (2 citations) HS dsDNA kit (2 citations)

2 protocols using qubit 1x dsdna hs assay kits

16S DNA Library Preparation with Optimized Cleanup

Cited 1 time

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16S library preparation for DNAs from all groups was performed per our published protocol.23 (link) An optimised clean-up step was performed with Agencourt AMPure XP beads using modifications proposed by Quail et al,24 (link) and 5 µL of the final Amplicon PCR product was used for the Index PCR, which was performed using the Nextera XT Index kit and 2 x KAPA HiFi HotStart Ready Mix, following the Illumina protocol. After a second modified clean-up step, the final product was quantified using Qubit ds DNA BR Assay kits and Qubit 1X dsDNA HS Assay kits on a Qubit 4 Fluorometer, and analysed using Agilent DNA 1000 chips (Agilent Technologies, Santa Clara, California, USA) and Agilent HS DNA chips (Agilent) on an Agilent 2100 Bioanalyzer System.

Oh S.J., Pimentel M., Leite G.G., Celly S., Villanueva-Millan M.J., Lacsina I., Chuang B., Parodi G., Morales W., Weitsman S., Singer-Englar T., Barlow G.M., Zhai J., Pichestshote N., Rezaie A., Mathur R, & Pimentel M. (2020). Acute appendicitis is associated with appendiceal microbiome changes including elevated Campylobacter jejuni levels. BMJ Open Gastroenterology, 7(1), e000412.

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16S Library Preparation for Small Bowel and Stool

Cited 2 times

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16S library preparation for DNAs from small bowel aspirates and stool was performed according to the Illumina (Illumina, San Diego, CA, USA) protocol https://support.illumina.com/documents/documentation/chemistry_documentation/16s/16s-metagenomic-library-prep-guide-15044223-b.pdf as described previously [15 (link)], using the gene-specific primers S-D-Bact-0341-b-S-17 and S-D-Bact-0785-a-A-21 published and validated by Klindworth et al. [16 (link)] to amplify the V3 and V4 regions. The final libraries were quantified using Qubit 1X dsDNA HS Assay kits on a Qubit 4 Fluorometer and analyzed using Agilent DNA 1000 chips (Agilent Technologies, Santa Clara, CA) on an Agilent 2100 Bioanalyzer System (Agilent Technologies, Santa Clara, CA).

Leite G.G., Weitsman S., Parodi G., Celly S., Sedighi R., Sanchez M., Morales W., Villanueva-Millan M.J., Barlow G.M., Mathur R., Lo S.K., Jamil L.H., Paski S., Rezaie A, & Pimentel M. (2020). Mapping the Segmental Microbiomes in the Human Small Bowel in Comparison with Stool: A REIMAGINE Study. Digestive Diseases and Sciences, 65(9), 2595-2604.

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