Arcobacter enrichment broth

The meat was sliced with a sterile scalpel in a petri dish and incubated for 18-24 h at 30°C in 10 mL Arcobacter enrichment broth (Oxoid, Basingstoke, England) under microaerophilic conditions (CampyGen sachets, Oxoid). Bacterial cells from the Arcobacter enrichment broth were subsequently cultured on Mueller Hinton agar (Oxoid) with 5% (vol/vol) sheep blood using a filter technique as described by Atabay et al. (Atabay et al., 2003 (link)). The plates were incubated at 35–37°C for 18–24 h under microaerophilic conditions. Plates showing no sign of bacterial growth were incubated for four additional days. The identity of oxidase-positive isolates was assessed by Matrix Assisted Laser Desorption Ionization Time of Flight mass spectrometry (MALDI-TOF MS; Bruker Daltonics GmbH & Co. KG, Bremen, Germany).