The chemicals used in this work are the following: Pluronic P123 (Sigma Aldrich), tetraethyl orthosilicate (TEOS) (Sigma Aldrich, 99%), (3-aminopropyl)trimethoxysilane (APTMS) (Sigma Aldrich, ≥97%), (3-chloropropyl)trimethoxysilane (CPTMS) (Sigma Aldrich, ≥97%), sodium azide (Fisher Chemical, ≥99%), ferrocene (Sigma Aldrich, 98%), ferrocenecarboxyaldehyde (Sigma Aldrich, 98%), ethynylferrocene (Angene, 98%), toluene (Stanlab, pure p.a.), absolute ethanol (CHEMPUR, 99.8%), dimethylformamide (DMF) (Fisher Chemical, ≥99%), tert-butyl alcohol (tBuOH) (Alfa Aesar, 99%), 2-propanol (iPrOH) (Stanlab, pure p.a.), copper(ii ) sulfate pentahydrate (Sigma Aldrich, ≥99%), sodium ascorbate (Sigma Aldrich, ≥98%), zinc chloride (Alfa Aesar, ≥98%, anhydrous), glacial acetic acid (CHEMPUR, 99.9%), hydrogen peroxide (Sigma Aldrich, 25–35% w/w in H2O, for ultratrace analysis). Deionized water was used throughout the experiments.
Tert butyl alcohol tbuoh
Manufactured by Thermo Fisher Scientific
Sourced in United States
Tert-butyl alcohol (tBuOH) is a chemical compound commonly used as a laboratory reagent. It is a colorless, flammable liquid with a characteristic odor. Tert-butyl alcohol is miscible with water and many organic solvents. It serves as a versatile solvent and chemical intermediate in various laboratory and industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Sodium azide, by Thermo Fisher Scientific (1 mentions)
Absolute ethanol, by Thermo Fisher Scientific (1 mentions)
Sodium ascorbate, by Merck Group (1 mentions)
Toluene, by EKF Diagnostics (1 mentions)
Dimethylformamide dmf, by Thermo Fisher Scientific (1 mentions)
Pluronic p123, by Merck Group (1 mentions)
Hydrogen peroxide, by Merck Group (1 mentions)
Ferrocene, by Merck Group (1 mentions)
Copper 2 sulfate pentahydrate, by Merck Group (1 mentions)
Perchloric acid, by Avantor (1 mentions)
Taurine, by Merck Group (1 mentions)
Titrinorm, by Avantor (1 mentions)
2 aminoethyl methacrylate hydrochloride aema, by Thermo Fisher Scientific (1 mentions)
Glycine, by Merck Group (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Fluorescein, by Merck Group (1 mentions)
Rmpi 1640, by Thermo Fisher Scientific (1 mentions)
P formaldehyde, by Merck Group (1 mentions)
Annexin 5 fitc propidium iodide apoptosis detection kit, by Elabscience (1 mentions)
4 protocols using tert butyl alcohol tbuoh
1
Synthesis of Ferrocene-Derived Materials
2
PVDF Membrane Fabrication and Characterization
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A commercially available polyvinylidene fluoride flat sheet microfiltration membrane (PVDF, ROTI®, 0.45 µm) was purchased from Carl Roth (Karlsruhe, Germany). In addition, the following chemicals were used: NaOH (1 M), perchloric acid (70%), and buffer solution pH 10 (TITRINORM®) from VWR (Radnor, PA, USA); tert-butyl alcohol (tBuOH, 99.5%) from Alfa Aesar (Karlsruhe, Germany); and nitrous oxide (N2O, 99.95%) from Air Liquide (Düsseldorf, Germany). Glycine, taurine, ethyl sodium sulfate (C2SS), dodecyl sodium sulfate (C12SS, SDS), tetramethylammonium bromide (C1TAB), and octyltrimethylammonium bromide (C8TAB) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Furthermore, 2-aminoethyl methacrylate hydrochloride (AEMA, 90%, stabilized) from Acros Organics (Geel, Belgium) was used. Deionized water of Millipore® quality and EtOH (absolute; Merck, Darmstadt, Germany) were used for all steps. All chemicals were of analytical grade and used without further purification.
3
Cytotoxicity Evaluation of Zinc-based Nanomaterial
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Imidazole-2-carboxaldehyde (2-ICA; 99% purity), zinc nitrate hexahydrate (Zn(NO3)2∙6H2O; 99% purity), polyvinylpyrrolidone (PVP; average mol. wt. = 40000), p-formaldehyde (95% purity), phosphate buffered saline (PBS), fluorescein (95% purity), arginine-glycine-aspartic acid (RGD), cisplatin (Cis; 99% purity), hydrochloric acids, and dimethyl sulfoxide (DMSO; 99.5% purity) were obtained from Sigma Aldrich. Methanol (99.9% purity) and tert-butyl alcohol (t-BuOH; 99% purity) were purchased from Fisher Scientific. RMPI 1640, fetal bovine serum (FBS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and trypsin were acquired from Gibco (Grand Island, USA). Hoechst33342 was procured from Invitrogen. Annexin V-FITC/propidium iodide (PI) apoptosis detection kit was received from Elabscience. A Milli-Q gradient water system was used to produce ultra-pure water. Normal human lung fibroblast cells (MRC-5 cells) and adenocarcinoma human alveolar epithelial cells (A549 cells) were obtained from the American Type Culture Collection (ATCC). All materials detailed herein were used without further purification.
4
Antimicrobial Efficacy of Thymol-Hydrogen Peroxide
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Thymol (CAS: 89-83-8, ≥98.5 %), hydrogen peroxide solution (CAS: 7722-84-1, 30 wt./wt. % in H 2 O containing stabilizer) and methanol (CAS: 67-56-1, >99.9 %, for HPLC) were purchased from Sigma-Aldrich. Phosphate buffered saline (PBS) (10X) was purchased from Fisher Scientific and diluted 10 times before use. Ethanol absolute anhydrous (64-17-5, RSE for electronic use) was purchased from Carlo Erba reagents. Tert-butyl alcohol (tBuOH) (≥99.0 %, CAS number: 75-65-0) was purchased from Fisher Scientific and used without any further purification. Liquid CO 2 (CAS: 124-38-9) was supplied by Air Liquide. Mueller-Hinton broth prepared from powder, Mueller-Hinton agar plates and pharmacopoeia diluent (NaCl peptone broth at pH 7) were purchased from VWR Chemicals. The cell proliferation reagent WST-1 for the spectrophotometric quantification of cell proliferation and viability was supplied by Roche.
A suspension of cellulose nanofibrils (Exilva P) at 2 wt. % solid content with a hemicellulose content of 2.8 %.was provided by Borregaard. The suspension was diluted to a 1% solid content with an Ultra Turrax IKA T25 stirrer at 8 000 rpm during 5 minutes.
The microorganism strains Staphylococcus epidermidis ATCC 14990, Escherichia coli ATCC 25922 and Candida albicans ATCC 14053 were supplied as KWIK-STIK lyophilized strains (Microbiologics).
A suspension of cellulose nanofibrils (Exilva P) at 2 wt. % solid content with a hemicellulose content of 2.8 %.was provided by Borregaard. The suspension was diluted to a 1% solid content with an Ultra Turrax IKA T25 stirrer at 8 000 rpm during 5 minutes.
The microorganism strains Staphylococcus epidermidis ATCC 14990, Escherichia coli ATCC 25922 and Candida albicans ATCC 14053 were supplied as KWIK-STIK lyophilized strains (Microbiologics).
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