Single cell RNA-sequencing libraries were generated with the Chromium Single Cell 3′ v2 (Day 7) and v3 (Day 14) assay (10X Genomics). Libraries were sequenced using the HighSeq 4000 platform (Illumina) to a depth of approximately 300 million reads per library with 2x50 read length. Raw reads were aligned to mouse genome (mm10) and cells were called using cellranger count (v3.0.2).
Chromium single cell 3 v2 day 7 and v3 day 14 assay
Manufactured by 10x Genomics
The Chromium Single Cell 3' v2 (Day 7) and v3 (Day 14) assays are single-cell RNA sequencing solutions developed by 10x Genomics. These assays capture and barcode individual cells, enabling high-throughput transcriptome analysis of thousands of single cells simultaneously. The core function of these products is to provide a platform for analyzing gene expression patterns at the single-cell level.
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Lab products found in correlation
2 protocols using chromium single cell 3 v2 day 7 and v3 day 14 assay
1
Single Cell RNA-seq of Mouse Cells
2
Single Cell RNA-seq of Mouse Cells
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Single cell RNA-sequencing libraries were generated with the Chromium Single Cell 3′ v2 (Day 7) and v3 (Day 14) assay (10X Genomics). Libraries were sequenced using the HighSeq 4000 platform (Illumina) to a depth of approximately 300 million reads per library with 2x50 read length. Raw reads were aligned to mouse genome (mm10) and cells were called using cellranger count (v3.0.2).
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