The antibodies anti-53BP1 (ab175933, 1:200 dilution), anti-RPA (ab2175, 1: 200 dilution), RAD51 (ab63801, 1:1000 dilution) and DNA2 (ab96488, 1:1000 dilution) were purchased from Abcam, USA. Antibodies anti-γH2AX (2577, 1:800 dilution), GAPDH (5174, 1:1000 dilution) were purchased from Cell Signaling, USA. BRCA1 (sc-6954) was purchased from Santa Cruz Biotechnology. Antibodies anti-SMARCAL1 (A301-616A, 1:1000 dilution), RPA2-pS4/S8 (A300-245A, 1:200 dilution), ZRANB3 (A303-033A, 1:1000 dilution) was purchased from Bethyl. BRCA2 (OP95, 1:500 dilution) was from Milipore. Antibodies specific for FEN1 (NB100-150, 1:1000 dilution), WRN (NBP1-31,895, 1:1000 dilution), HLTF (NB100-280, 1:2000 dilution) were purchased from Novus Biologicals (USA). Mouse anti-BrdU (347,580, 1:40) was purchased from BD Biosciences. AF647 (A-21247, 1:1000) and AF488 (A-11001, 1:1000) were purchased from ThermoFisher Scientific. DNA-PKcs inhibitor: NU-7441 (HY-11006) were purchased from MedChem Express (USA). MRE11 inhibitor: mirin (M9948) was purchased from Sigma-Aldrich.
Rad51 ab63801
Manufactured by Abcam
Sourced in United States
RAD51 (ab63801) is a recombinant protein that functions as a key component of the DNA repair pathway. It plays a crucial role in homologous recombination, a process that is essential for the maintenance of genomic stability. This protein is widely used in various research applications, including the study of DNA damage response and DNA repair mechanisms.
Automatically generated - may contain errors
Lab products found in correlation
Gapdh, by Cell Signaling Technology (1 mentions)
Af647 a 21247, by Thermo Fisher Scientific (1 mentions)
Af488 a 11001, by Thermo Fisher Scientific (1 mentions)
Anti γh2ax 2577, by Cell Signaling Technology (1 mentions)
Brca1 sc 6954, by Santa Cruz Biotechnology (1 mentions)
Anti 53bp1 ab175933, by Abcam (1 mentions)
Nb100 150, by Novus Biologicals (1 mentions)
Cleaved caspase 3 9661, by Cell Signaling Technology (1 mentions)
Flag tag (flag), by Merck Group (1 mentions)
Ku70 10723 1 ap, by Proteintech (1 mentions)
γh2ax 05 636, by Merck Group (1 mentions)
Ect2 07 1364, by Merck Group (1 mentions)
Olaparib s1060, by Selleck Chemicals (1 mentions)
3 protocols using rad51 ab63801
1
DNA Damage Response Protein Analysis
2
Antibody Immunodetection Protocol
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Antibodies to detect PMVK (HPA029900), MRE11(HPA002691) and RPA1 (HPA006914) were obtained from Atlas Antibodies. Alpha‐tubulin (#2125) and cleaved caspase‐3 (#9661) antibodies were purchased from Cell Signaling Technology. Anti‐beta‐actin (A5441), anti‐Flag (F1084), Hoechst 33342 stain (B2261), and cycloheximide (O1810) were purchased from Sigma‐Aldrich. NBS1 antibody (NB100‐143) was obtained from Novus Biologicals. Ku80 antibody (#556429) was purchased from BD Biosciences. Anti‐DNA‐PKCS (sc‐9051) was purchased from Santa Cruz Biotechnology. PARP (ab32071) and Rad51(ab63801) antibodies were obtained from Abcam. Anti‐gamma‐H2AX (A300‐081A) was obtained from Bethyl Laboratories, Inc. HA antibody (ADI‐MSA‐106) was purchased from Enzo. Alexa 555‐conjugated anti‐rabbit IgG (A21429) was obtained from Invitrogen. Anti‐rabbit IgG horseradish peroxidase (711‐036‐152) and mouse IgG (711‐036‐151) were purchased from Jackson ImmunoResearch Laboratories, Inc. MG132 (474790) was obtained from Calbiochem.
3
Antibody Sources and Research Chemicals for Cell Studies
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The sources of antibodies against the following proteins were as follows: ECT2 (07-1364, for Western blotting [WB], ChIP, and IF) and γH2AX (05-636, for IF and WB) were purchased from Millipore; poly(ADP-ribose) (ALX-840-220-R100, for IF) was purchased from ENZO; HA (sc-805, for WB) and PARP1 (sc-7150 for WB) were purchased from Santa Cruz Biotechnology; β-actin (AC026, for WB) was purchased from ABclonal; FLAG (F1804, for IP and WB) was purchased from Sigma; RAD51 (ab63801, for IF and WB) and RAD50 (ab89, for WB) were purchased from Abcam; BRCA1 (22362-1-AP, for IF and WB), KU70 (10723-1-AP, for WB), and KU80 (16389-1-AP, for WB) were purchased from Proteintech; H3 (YM3038, for WB), H2AX (YT2155, for WB), and LaminA/C (YT2521, for WB) were purchased from ImmunoWay. Anti-FLAG M2 affinity gel (A2220), 3× FLAG peptide (F4799), puromycin (P8833), 4-OHT (H6278), KU55933 (SML1109), and NU7026 (N1537) were purchased from Sigma. Olaparib (S1060) was purchased from Selleck. MNNG (70-25-7) was purchased from Tokyo Chemical Industry.
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