Dapi 4 6 diamidino 2 phenylindole dihydrochloride acridine orange

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is the product of SRL, N-acetyl cysteine (NAC) was purchased from Sigma Aldrich, and HCT116 and HEK293 cell lines were obtained from the National Centre For Cell Science (NCCS), Pune, Govt. of India. HCT 116 cell lines were cultured in DMEM Dulbecco's Modified Eagle's Medium (DMEM), supplemented with 10% fetal bovine serum (FBS) and 1% penicillin and streptomycin antibiotics, where trypsin and ethylenediaminetetraacetic acid (EDTA) were all purchased from Gibco BRL. Tissue culture plastic wares were obtained from NUNC (Roskidle, Denmark). DAPI (4′,6-diamidino-2-phenylindole dihydrochloride), acridine orange (AO), and ethidium bromide (EtBr) were obtained from Invitrogen (California) and BCA protein assay reagent from Fermentus (EU). The primary antibodies for Bcl2, Bax, caspase 3, cleaved caspase-3, caspase-9, cleaved caspase-9, cytochrome c, P53, cleaved PARP, and β-actin were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). DCF-DA was obtained from Sigma-Aldrich (MO, USA). An Annexin-V FITC apoptosis detection kit was obtained from Calbiochem (CA, USA). Doxorubicin was purchased from Sigma Aldrich.

MTT and DMSO were purchased from Sigma Chemical Co. (St. Louis, MO, USA); p-NBT-BCIP systems were from Amresco (Solon, OH, USA); RPMI 1640, FBS (Fetal Bovine Serum), and penicillin—streptomycin—neomycin were from Gibco-BRL (Grand Island, NY, USA); tissue-culture plasticware was from Nunc (Roskilde, Denmark); Bradford protein assay reagent was from Fermentas (Pittsburgh, PA, USA); DAPI (4',6-diamidino-2-phenylindole dihydrochloride), acridine orange (AO), and ethidium bromide (EtBr) were from Invitrogen (Carlsbad, CA, USA); rabbit and goat anti-BAX, BAD, BCL-2, p38, p-P38, ERK, p-ERK, JNK polyclonal and secondary antibodies in alkaline phosphatase, and FITC and PE-conjugated were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).