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Jsm 7001f sem

Manufactured by JEOL
Sourced in Japan

The JEOL JSM-7001F is a scanning electron microscope (SEM) that offers high-resolution imaging capabilities. The SEM utilizes an electron beam to scan the surface of a sample, providing detailed information about its topography and composition. The JSM-7001F is designed to deliver reliable and accurate results for a wide range of applications.

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7 protocols using jsm 7001f sem

1

Multimodal Characterization of Materials

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Scanning electron microscope (SEM) images were taken from JEOL JSM-7001F SEM instruments, Tokyo, Japan at 3 kV. Transmission electron microscope (TEM) images were obtained from FEI Tecnai g2t20 at 200 kV. Raman spectra were recorded on an INVIA Raman microprobe (Renishaw Instruments, London, UK) with a 514 nm laser excitation. X-ray photoelectron (XPS) characterizations were carried out on a Thermo ESCALAB 250i X-ray photoelectron spectrometer. X-ray diffraction (XRD) data were measured on a Rigaku D-MAX 2500/PC using Cu Kα radiation (λ = 1.54056 Å).
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2

Multimodal Structural Characterization

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A JEOL-JSM-7001F SEM and JEOL 2100 field emission gun TEM were used to examine the morphology. AC HAADF-STEM images and EDS elemental mappings were carried out by a Cs-corrected FEI Titan G2 60-300 equipped with a Super-X EDS detector and operated at 300 kV. Chemical compositions and elemental oxidation states were checked by XPS spectra (PHI-5702). X-ray absorption spectra (XANES and EXAFS) of W L3- and Mo K-edges were measured on VESPERS beamline at the Canadian Light Source, by scanning a double-crystal Si(111) monochromator and collecting emitted x-ray fluorescence.
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3

Ultrastructural Analysis of Trophozoites Treated with Ciliate Sinensis

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Trophozoites treated with 100% C. sinensis were harvested, fixed, and dehydrated using a graded series of ethanol as described below for TEM. After dehydration, critical point drying was performed by infiltrating the samples in hexamethyldisilazane (HMDS) (Acros, Germany) for 5 min twice to further enhance drying of the samples. The samples were mounted on aluminium SEM stubs with a double-sided carbon sticker and sputter coated with gold prior to SEM imaging. Images were obtained using a JEOL JSM 7001F SEM (JEOL, Ltd., Tokyo, Japan).
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4

Nanowire LED Structural Characterization

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The starting sample used in this study was a typical MQW LED structure consisting of layers in the order from top to bottom: a 0.2 μm p-type GaN:Mg top layer, then 5 periods of In0.2Ga0.8N/GaN MQWs with barrier’s (well’s) thickness of 30 nm (3 nm), and a 3.0 μm n-type GaN:Si buffer bottom layer, which was grown on c-plane sapphire with metalorganic chemical vapor deposition. The nanowires were prepared with the “top-down” dry etching process using Ni self-assembled nanoislands as the etching mask. Other preparation details of the NWs can be referred to a publication24 .
A JEOL (JSM-7001F) SEM microscopy was employed to characterize the dimensions of the nanopillars. Micro-Raman scattering spectra were measured on an integrated WITec Alpha scanning confocal micro-Raman system with the back-scattering geometric configuration14 . In the Raman scattering measurements, the 514.5 nm line of an argon ion gas laser was used as the excitation source. Variable-temperature PL spectral measurements were carried out on a home-made PL system using a Kimmon 325 nm He-Cd laser as the excitation light source. The maximum output power of the He-Cd laser was 38 mW. In the PL measurements, the samples were mounted on the cold figure of a Janis closed cycle cryostat proving a varying temperature range of 3.6–300 K. A detailed description of the PL setup can be referred to a previous publication25 .
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5

Pollen Morphology of Pelargonium Species

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While Pelargoniumendlicherianum was collected from Hakkari in the C9 grid of Flora of Turkey, Pelargoniumquercetorum was collected from Tunceli in the B7 grid of Flora of Turkey. The pollen characteristics of these 2 species were examined in preparations by the method of Woodhouse (1935) for the LM. The polar and equatorial axis, colpus length and width, and exine and intine thickness of the pollen were measured 30 times. The morphological characteristics under the LM were measured using an Olympus BX41 microscope. Microphotographs were taken from this microscope’s camera. During SEM, selected dry samples of pollens were placed on the aluminum stabs with the help of double-sided adhesive tape and coated in gold with a vacuum. The images of the pollen were taken with a Jeol JSM 7001-F SEM in the SEM laboratory of the Department of Biology, Fırat University.
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6

Characterization of Adsorbent Materials

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The prepared adsorbents were characterized
by various techniques
as follows. The distribution of MS particle diameters before and after
ball milling was measured by a SALD-7101 LDPAZ (Japan). The crystalline
structure was characterized using a Bruker D8-Focus X-ray diffractometer
by applying monochromated Cu Kα radiation (λ = 0.15418
nm) in the 2θ range of 20–80 ° at 2°·min–1. The morphology and elemental composition were analyzed
by a JSM-7001F SEM (JEOL, Japan) equipped with and EDS (Oxford, Britain)
operating at 20 kV. XPS (ESCALAB 250Xi, Thermo Fisher Scientific,
USA) was used to measure the chemical composition and the binding
energy of Zr 3d before and after P adsorption. A VSM (Lake Shore 7400,
USA) was used to measure the room-temperature magnetism of the magnetic
adsorbents. BET surface areas and pore size distributions were determined
through N2 adsorption/desorption over a Micromeritics ASAP
2010 system (Micromeritics, USA) at 77 K. A ZetaProbe 7020 Zeta-Potential
meter was used to analyze the zeta potential. A FTIR (Thermo Scientific
Nicolet iS50, USA) was used to analyze functional groups on the adsorbents
in the region of 4000–400 cm–1.
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7

Visualizing Bacteriocin's Action on E. coli

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The mode of action of purified bacteriocin on E. coli cells was visualized by scanning electron microscopy (SEM). The exponentially grown cells with OD 0.2 at 600nm were incubated with the purified bacteriocin at 37°C for 20 hr. The treated bacterial cells were centrifuged and washed with 1mM phosphate buffer saline PBS at pH 7. The samples were fixed in 8% glutaraldehyde followed by gradual dehydration. The morphological changes were visualized under JEOL JSM-7001F SEM (Chopra, 2015) (link).
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