Sera mag speedbead carboxylate modified magnetic particles hydrophobic

Manufactured by GE Healthcare

Sera‐Mag SpeedBead Carboxylate‐Modified Magnetic Particles (Hydrophobic) are uniform superparamagnetic beads with a carboxylate modified surface. They can be used for various biomolecule separation and purification applications.

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Lab products found in correlation

Superscript 2 reverse transcriptase, by Thermo Fisher Scientific (2 mentions) Rnase inhibitor, by Thermo Fisher Scientific (2 mentions) Oligo d t 18 primer, by New England Biolabs (2 mentions) Kingfisher duo instrument, by Thermo Fisher Scientific (2 mentions)

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Sera-Mag SpeedBeads (13 citations) Sera-Mag SpeedBead Carboxylate-Modified Magnetic Particles (5 citations) Sera-Mag Magnetic SpeedBeads (4 citations) Sera-Mag Carboxylate-Modified Magnetic SpeedBeads (3 citations) SpeedBead Magnetic Carboxylate Modified Particles (3 citations) SpeedBeads (2 citations)

2 protocols using sera mag speedbead carboxylate modified magnetic particles hydrophobic

Pro-B Cell RNA Isolation and qPCR

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Total RNA was prepared from ex vivo sorted pro‐B cells by using a semi‐automated RNA bead isolation method with Sera‐Mag SpeedBead Carboxylate‐Modified Magnetic Particles (Hydrophobic; GE Healthcare) run on the magnetic particle processor KingFisher Duo instrument (Thermo Fisher Scientific). The cDNA was synthesized using Oligo d(T)₁₈ primer (NEB) and SuperScript® II Reverse Transcriptase (Thermo Fisher Scientific) in the presence of RNase inhibitor (Thermo Fisher Scientific). Transcripts of the Gfp gene were amplified by qPCR using primers located in different exons (Dataset EV2) and normalized against the Tbp mRNA.

Hill L., Jaritz M., Tagoh H., Schindler K., Kostanova‐Poliakova D., Sun Q., Schwickert T.A., Leeb M, & Busslinger M. (2023). Enhancers of the PAIR4 regulatory module promote distal VH gene recombination at the Igh locus. The EMBO Journal, 42(15), e112741.

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Multiplex Transcriptome Analysis from B and T Cells

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Total RNA was prepared from distinct B and T cell types sorted from the bone marrow, thymus and spleen or from in vitro cultured v-Abl pro-B cell lines, using a semi-automated RNA bead isolation method with Sera-Mag SpeedBead Carboxylate-Modified Magnetic Particles (Hydrophobic; GE Healthcare) run on the magnetic particle processor KingFisher Duo instrument (Thermo Fisher Scientific). The cDNA was synthesized using Oligo d(T)₁₈ primer (NEB) and SuperScript II Reverse Transcriptase (Thermo Fisher Scientific) in the presence of RNase inhibitor (Thermo Fisher Scientific). The transcripts of selected genes were amplified by qPCR using primers located in different exons (Supplementary Table 4) and were normalized against the Tbp mRNA.

Hill L., Ebert A., Jaritz M., Wutz G., Nagasaka K., Tagoh H., Kostanova-Poliakova D., Schindler K., Sun Q., Bönelt P., Fischer M., Peters J.M, & Busslinger M. (2020). Wapl repression by Pax5 promotes V gene recombination by Igh loop extrusion. Nature, 584(7819), 142-147.

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