Pancreases were harvested from mice at indicated ages and fixed in 10% formalin overnight at room temperature. Fixed tissue was processed into paraffin blocks and cut into 5 μM sections using a Leica RM2235 microtome. Tissue sections were deparaffinized in a xylene and ethanol series and then washed with water. Antigen retrieval was performed by incubating slides in Target Retrieval Buffer (DAKO, #S1699) in a pressure cooker. Immunohistochemical analysis was performed using the EnVision+ HRP Kit (DAKO, #401111) and DCLK1 (Cell Signaling, #62257) according the manufacturer’s instructions. Slides were counterstained with hematoxylin, dehydrated through an ethanol and xylene series, and coverslips were mounted using Permount (Fisher, #SP15–100). Images were acquired using an Olympus BX-UCB slide scanner.
Bx ucb slide scanner
Manufactured by Olympus
The BX-UCB slide scanner from Olympus is a high-performance digital slide imaging solution designed for a variety of laboratory applications. It captures high-resolution digital images of microscope slides, enabling efficient storage, analysis, and sharing of specimen data.
Automatically generated - may contain errors
Lab products found in correlation
Envision hrp kit, by Agilent Technologies (3 mentions)
Target retrieval buffer, by Agilent Technologies (2 mentions)
Rm2235 microtome, by Leica (2 mentions)
Dclk1, by Cell Signaling Technology (2 mentions)
Permount, by Thermo Fisher Scientific (2 mentions)
Antibody diluent, by Agilent Technologies (1 mentions)
3 protocols using bx ucb slide scanner
1
Immunohistochemical Analysis of DCLK1 in Murine Pancreas
2
Immunohistochemical Analysis of DCLK1 in Murine Pancreas
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Pancreases were harvested from mice at indicated ages and fixed in 10% formalin overnight at room temperature. Fixed tissue was processed into paraffin blocks and cut into 5 μM sections using a Leica RM2235 microtome. Tissue sections were deparaffinized in a xylene and ethanol series and then washed with water. Antigen retrieval was performed by incubating slides in Target Retrieval Buffer (DAKO, #S1699) in a pressure cooker. Immunohistochemical analysis was performed using the EnVision+ HRP Kit (DAKO, #401111) and DCLK1 (Cell Signaling, #62257) according the manufacturer’s instructions. Slides were counterstained with hematoxylin, dehydrated through an ethanol and xylene series, and coverslips were mounted using Permount (Fisher, #SP15–100). Images were acquired using an Olympus BX-UCB slide scanner.
3
Immunohistochemical Analysis of Ki67 Expression
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For immunohistochemistry, deparaffinized sections were subjected to antigen retrieval and processed with the EnVision + HRP kit (K401111-2, DAKO, Glostrup, Denmark) according to the manufacturer’s instructions. A primary polyclonal antibody against Ki67 (Cell Signaling #12202) at 1:400 dilution was diluted in Antibody Diluent (DAKO #S0809) and incubated overnight at 4°C. Next, sections were incubated in the provided anti-rabbit HRP-labeled polymer reagent, and detection was performed according to the manufacturer’s protocol. Images were acquired using an Olympus BX-UCB slide scanner.
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