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X vivo 15 serum free cell medium

Manufactured by Lonza
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X-VIVO 15 is a serum-free cell culture medium formulated for the growth and maintenance of a variety of cell types. It provides the necessary nutrients and growth factors to support cell proliferation and viability in an animal component-free environment.

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Lab products found in correlation

Cellbind surface, by Corning (2 mentions) Dulbecco s phosphate buffered saline dpbs, by Merck Group (2 mentions)

Close spelling variants of this product

X-VIVO 15 serum-free cell-culture medium X-VIVO 15 Chemically Defined Serum-free Hematopoietic Cell Medium X-VIVO 15 Serum-free Hematopoietic Cell Medium X-VIVO 15 serum-free medium X-VIVO serum-free medium X-Vivo 15 cell medium X-vivo 15 serum X-VIVO 15 X-VIVO medium Serum-free medium

2 protocols using x vivo 15 serum free cell medium

1

CTL Enrichment via Plastic Adherence

Cited 1 time
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In order to minimize the presence of non-specific cells in the cellular product, the sample was enriched for CTLs by a plastic adherence process [16 (link)]. In brief, 2.25 × 108 cells were plated in sterile 225 cm2 A/N flasks with CellBIND Surface (Corning, Corning, NY) at 0.5 × 106 cells/ml in X-VIVO 15 Serum-free cell medium w/o supplements (Lonza). Cells were incubated for 1 h at 37 °C and 5 % CO2. Non-adherent cells were carefully collected by aspiration to avoid the disruption of adherent myeloid cellular populations and were washed with Dulbecco’s phosphate buffered saline (dPBS; Sigma-Aldrich) before quantification.
Beloki L., Ciaurriz M., Mansilla C., Zabalza A., Perez-Valderrama E., Samuel E.R., Lowdell M.W., Ramirez N, & Olavarria E. (2015). Assessment of the effector function of CMV-specific CTLs isolated using MHC-multimers from granulocyte-colony stimulating factor mobilized peripheral blood. Journal of Translational Medicine, 13, 165.
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2

Adherent Cell Enrichment Protocol

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2.25 × 107 cells were suspended in 45 ml of X-VIVO 15 Serum-free cell medium w/o supplements (Lonza, Basel, Switzerland) in a sterile 225 cm2 A/N flask with CellBIND Surface (Corning, Corning, NY) for 1 hour at 37°C and 5% CO2. Non-adherent cells were carefully collected by aspiration to avoid the disruption of the adherent cellular populations. Obtained cells were washed with Dulbecco’s phosphate buffered saline (dPBS, Sigma-Aldrich, St. Louis, MO) before quantification and cytometric analysis.
Beloki L., Ciaurriz M., Mansilla C., Zabalza A., Perez-Valderrama E., Samuel E.R., Lowdell M.W., Ramirez N, & Olavarria E. (2014). CMV-specific T cell isolation from G-CSF mobilized peripheral blood: depletion of myeloid progenitors eliminates non-specific binding of MHC-multimers. Journal of Translational Medicine, 12, 317.
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