The mice were sacrificed on the 14th day after treatment. The tumor tissues and major organs (heart, liver, lung, kidney and spleen) were separated and collected. The tissue slices were stained at room temperature with hematoxylin (2 min) and eosin (1 min). (H&E), and the histological changes of tissues were observed via an Eclipse Ti light microscope (magnification, ×40; Nikon Corporation).
Eclipse ti light microscope
Manufactured by Nikon
Sourced in Japan
The Eclipse Ti is a high-performance light microscope designed for advanced microscopy applications. It features a modular design that allows for the integration of various imaging techniques, including brightfield, phase contrast, and fluorescence microscopy. The Eclipse Ti is equipped with a sensitive camera and advanced optics to deliver high-quality images for research and analysis purposes.
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Lab products found in correlation
4 protocols using eclipse ti light microscope
1
Histological Analysis of Tumor and Organ Tissues
2
Histological Analysis of Colon Tissue
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To perform a histological analysis of the tissue samples, 10% buffered formalin-fixed colon tissues were dehydrated and embedded in paraffin blocks. After sectioning, the slices were deparaffinized and rehydrated using a xylene-ethanol-water gradient system. The sections were stained with hematoxylin and eosin (H&E) to confirm iron accumulation. For the morphological analysis using the Perls’ Prussian blue staining, sliced samples were incubated in a working solution of 1:1 20% HCl and 10% potassium ferrocyanide. After washing with distilled water, the slices were stained with a nuclear fast red solution. All representative photomicrographs of the samples were obtained using an Eclipse Ti light microscope (Nikon, Tokyo, Japan).
3
Imaging Cardiac Action Potential Duration
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iPSC-CMs were cultured on 35-mm glass-bottom dishes (MatTek, Ashland, MA; P35G-1.5-10-C) that were precoated with Matrigel matrix at 37°C, 5% CO2. For imaging, cells were incubated at 37°C, 5% CO2 for 20 minutes in Tyrode solution containing a volage-sensitive fluorescent dye, FluoVolt, that responds to changes in membrane potential (Thermo Fisher Scientific; F10488). The cells were then washed 3 times in fresh Tyrode solution. During imaging, the dishes were kept in a heated 37°C stage-top environment chamber supplied with 5% CO2. Imaging of voltage-indicated cellular APD was taken under a ×40 water objective using a Nikon Eclipse Ti light microscope (Nikon, Tokyo, Japan). Time-lapse videos of multiple, individual beating iPSC-CMs, paced at 1 Hz were recorded at a speed of 20 ms per frame for 20 seconds at 10% LED power. Single regions of interest were selected for every beating iPSC-CM captured in the recordings. The raw data were exported to Excel software (Microsoft, Redmond, WA) and then analyzed with an “in-lab” developed Excel-based program.11
4
Immunohistochemical Analysis of HIF-1α Expression
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The dissected tissue was fixed in 4% polyoxymethylene for two days, decalcified in 10% EDTA at 48°C for 2 weeks and sliced into 5-µm sections. Sections were subjected to antigen retrieval in sodium citrate buffer (1 M, pH 6.0) at 99°C for 20 min and incubated with primary antibody against HIF-1α (1:1,000; cat. no. RM242; Boster Biological Technology) overnight at 4°C. The slides were then incubated with a biotin-conjugated goat anti-rabbit secondary antibody (1:1,000; cat. no. BA1003; Wuhan Boster Biological Engineering Technology Co., Ltd.) at room temperature for 45 min. The slides were examined with a Nikon Eclipse Ti light microscope under a ×40 objective (Nikon Instruments). A positive expression for HIF-1α was detected when brownish-yellow particles appeared in the nucleus, membrane or cytoplasm. Results of the IHC were analyzed using semi-quantitative scoring method as described by Lin et al (34 (link)). Briefly, staining was scored as negative (−), mild (+), moderate (++) or strong (+++) for <1, 1–10, 10–50 or >50% of cell nuclear stain, respectively. In the present study, moderate (++) and strong (+++) scores defined high HIF-1α expression, whereas negative (−) and mild (+) scores defined low HIF-1α expression.
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