UV absorbance was measured on a Shimadzu UV-1800 spectrophotometer. Size exclusion chromatography (SEC) was run on Shimadzu UFLC system. Agarose gel was run on the Bio-Rad horizontal electrophoresis system (Wide Mini-Sub Cell GT Cell). AFM images were obtained on a Bruker Multimode 8 SPM equipped with a liquid cell. DLS were undertaken on Malvern Zetasizer Nano ZSP instrument. Real time fluorescent spectrum was obtained on Shimadzu RF-5301 Spectrofluorophotometer with the software Labsolutions RF.
Wide mini sub cell gt cell
Manufactured by Bio-Rad
Sourced in United States
The Wide Mini-Sub Cell GT Cell is a piece of laboratory equipment designed for agarose gel electrophoresis. It provides a platform for the separation and analysis of DNA, RNA, or protein samples based on their molecular size and charge. The core function of this device is to facilitate the electrophoretic separation of macromolecules within an agarose gel matrix.
Automatically generated - may contain errors
Lab products found in correlation
Uflc system, by Shimadzu (1 mentions)
Multimode 8 spm, by Bruker (1 mentions)
Zetasizer nano zsp instrument, by Malvern Panalytical (1 mentions)
Uv 1800 spectrophotometer, by Shimadzu (1 mentions)
Rf 5301 spectrofluorophotometer, by Shimadzu (1 mentions)
Sd0041, by Thermo Fisher Scientific (1 mentions)
3 protocols using wide mini sub cell gt cell
1
Characterization of Biomolecular Structures
2
Chrysosplenetin's DNA Cleavage Activity
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The supercoiled pBR322 plasmid DNA (Thermo Scientific, SD0041) hydrolytic cleavage activity of chrysosplenetin was examined using agarose gel electrophoresis (Bio-Rad, Wide Mini-Sub Cell GT Cell). In this work, the stock solution of chrysosplenetin at 10 mM was prepared in DMSO and diluted to working solutions (12.5, 25, 50, 100 and 200 µM) with buffer (50 mM Tris-HCl (pH 7.0)). Control experiments were done in the presence of DMSO (2%). Chrysosplenetin was treated with supercoiled pBR322 plasmid DNA at 37 °C for 1 h in the buffer. After incubation, loading buffer (bromophenol blue, xylene cyanol, glycerol, EDTA, SDS) was added and the reaction mixtures were loaded on agarose gel (0.8%) with EB staining for 90 min at 100 V in TAE (Tris-acetic acid-EDTA) buffer. After electrophoresis, band intensities were photographed and calculated using BioRad Gel Doc XR system and Image Lab Version 4.0.1 Software program [19] .
3
Topological Isoform Separation of pLENSO/Zeo
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We isolated topological isoforms of pLENSO/ Zeo by loading 300 ng of undigested plasmid beside 150 ng of NotI digested plasmid onto a 1.0% agarose slab gel. Gel electrophoresis was performed in TAE buffer (20 mM Tris, 10 mM acetic acid and 0.5 mM EDTA, pH=8.0) at a constant voltage of 60 V at room temperature using the gel electrophoretic unit, Wide Mini-Sub Cell GT Cell (Bio-Rad Laboratories, USA). The gel was stained with ethidium bromide and visualized by UV light. The gel image was captured using a transilluminator, UVITEC ESSENTIAL V2 (UVItec, UK) that contained a charge-coupled device (CCD) grade camera. The intensity of the resultant DNA bands was quantified by optical densitometry using NIH ImageJ software version 1.48 (http://rsb.info.nih.gov/ij/).
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