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2 protocols using anti β mhc

1

Mitochondrial Dynamics in Metabolic Disorders

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Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco (Big Cabin, OK, USA). Collagenase and trypsin were purchased from Sigma (St. Louis, MO, USA). Iso and metformin were purchased from Sigma. The following antibodies were used in this study: anti-Mfn2, anti-PINK1 (Abcam, Cambridge, UK), anti-Beclin1, anti-P62, anti-LC3B, anti-PGC 1α, anti-TFAM, anti-NRF1, anti-ANP, anti-β-MHC, anti-Col-1, anti-TGF-β-1, anti-GAPDH and anti-β-actin (Proteintech, Chicago, IL, USA).
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2

Protein Extraction and Quantification

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Proteins were extracted, quantified and analyzed as reported previously (Xiong et al. 2018 (link); Liu et al. 2019 (link); An et al. 2021 (link)). The primary and secondary antibodies used included anti-β-MHC, anti-ANP, anti-HO1, anti-GPX4 (1:1000, Proteintech, USA), anti-PINK1 (1:200, Santa Cruz, USA), anti-SIRT1 (1:1000, Cell Signaling Technology, USA), goat anti-rabbit and anti-mouse IgG-HRP (1:5000, Fudebio, China) antibodies.
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