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4 protocols using cetyl palmitate

1

Formulation and Characterization of Tetracaine Delivery

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Tetracaine base (TTC) was purchased from AK Scientific, Inc. (Union City, USA). The surfactants Pluronic F68 (P68) and Tween 80 were supplied by Sigma (St. Louis, USA); cetyl palmitate (CP) and Dhaykol 6040 (DK)—or capric/caprylic triglycerides—by Dhaymers Quím. Fina (Taboão da Serra, Brazil); myristyl myristate (MM) by Croda (Campinas, Brazil) and diethylene glycol monoethyl ether or Transcutol (TRANS) by Gattefossé (Lyon, France). HPLC-grade acetonitrile and methanol were purchased from J.T. Baker (Allentown, USA). 3T3 cells were from American type culture collection (ATCC, Manassas, VA, USA). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), RPMI medium, fetal bovine serum, penicillin, streptomycin and uranyl acetate were purchased from Sigma-Aldrich (Missouri, USA). All other reagents were of analytical grade. Deionized water (18 MΩ) was obtained from an Elga USF Maxima ultra-pure Water purifier.
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2

Development of Nanoemulsion Formulations

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Rosmarinic acid (RA), polysorbate 20 (Tween 20®), polysorbate 80 (Tween 80®), steareth-10 (Brij S10®), polyoxyethylene mono (octylphenyl) ether (Triton X-114®), decyl glucoside (Plantacare 2000®), polyethylene glycol 400 (PEG-400), propylene glycol, glycerin, cholesterol, stearic acid, glyceryl monostearate, cetyl palmitate, and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tea seed (Camellia oleifera Abel.) oil, avocado (Persea americana Mill.) oil, and almond (Prunus dulcis Mill.) oil were cosmetic grade purchased from Namsiang (Chiang Mai, Thailand). HPLC grade acetonitrile was purchased from Merck (Darmstadt, Germany). Ethanol, ethyl acetate, n-hexane, and dimethyl sulfoxide (DMSO) were analytical grade purchased from Labscan (Dublin, Ireland).
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3

Formulation and Characterization of Sunscreen Nanoparticles

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Parsol® MCX (2-ethylhexyl 4-methoxycinnamate or octyl methoxycinnamate) was purchased from Acofarma (Barcelona, Spain). Tetraethyl orthosilicate (TEOS, 98%) was purchased from Merck (Darmstad, Germany). Cetyl palmitate (solid lipid) was purchased from Sigma-Aldrich (Portugal). Tego Care® 450 (surfactant) was obtained from Evonik Industries (Germany). Carbopol® 971P NF Polymer was purchased from Lubrizol (Wickliffe, USA). MilliQ Plus system (Millipore, Germany) was used as source of water of high purity used in the study.
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4

Preparation of Opuntia humifusa Cladodes

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Fresh O. humifusa cladodes, from plants grown for 3 years, were collected from Uiryeong-gun, Gyeongsangnam-do, Korea. Zinc chloride (ZnCl2) and ethanol were purchased from Daejung Chemicals & Metals Co. Ltd. (Siheung-si, Gyeonggi-do, Republic of Korea). APTES was purchased from Tokyo Chemical Industry (Nihonbashi-honcho, Chuo-ku, Tokyo, Japan). Folin-Denis reagent, sodium carbonate (Na2CO3), glycerol, and cetyl palmitate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate buffered saline (PBS) was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). A Milli-Q Millipore filter system (Millipore Co., Billerica, MA, USA) was used to obtain distilled (DI) water. Potassium bromide (KBr) was procured from PIKE Technologies (Fitchburg, WI, USA).
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