Cholesterol and E2 were purchased from Sigma-Aldrich (St. Louis, MO), cholesterol sulfate from Avanti Polar Lipids (Birmingham, AL), and 17-AAG from Enzo Life Science (Farmingdale, NY). All chemicals except cholesterol (using ethanol) were prepared in dimethyl sulfoxide, stored as small aliquots at − 20 °C, and then diluted as needed in cell culture media. DMEM media were purchased from HyClone (Logan, UT). FBS and charcoal-stripped FBS were purchased from Tissue Culture Biologicals (Long Beach, CA), Neon transfection system from ThermoFisher Scientific (Waltham, MA), and D-Plus ECL solution from Dongin LS (Seoul, Korea). Anti-STS polyclonal antibody (ab62219), anti-Involucrin polyclonal antibody (ab53112), and anti-Loricrin polyclonal antibody (ab85679) were purchased from Abcam (Cambridge, MA). β-Actin (sc-4778), goat anti-rabbit IgG-Texas Red (sc-2780), and Ultra Cruz mounting medium (sc-24941) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-ERα polyclonal antibody (A0296) was purchased from Abclonal (Woburn, MA) and anti-YPEL3 polyclonal antibody (15403-1-AP) from Proteintech (Chicago, IL). All other chemicals were obtained from commercial sources.
17 aag
Manufactured by Enzo Life Sciences
Sourced in United States
17-AAG is a small molecule compound that functions as a heat shock protein 90 (Hsp90) inhibitor. Hsp90 is a molecular chaperone protein involved in the folding and stabilization of various client proteins. 17-AAG binds to and inhibits the Hsp90 protein, which can lead to the degradation of Hsp90 client proteins.
Automatically generated - may contain errors
Lab products found in correlation
Auy922, by Enzo Life Sciences (2 mentions)
Ex 527, by Abcam (2 mentions)
β actin sc 4778, by Santa Cruz Biotechnology (1 mentions)
Neon transfection system, by Thermo Fisher Scientific (1 mentions)
Fitc mouse anti human cd44, by BD (1 mentions)
Rpmi medium, by Thermo Fisher Scientific (1 mentions)
Heat inactivated fbs, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
3 protocols using 17 aag
1
Investigating Cholesterol and Steroid Signaling
2
Multidrug-Resistant Cancer Cell Lines
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CEM human lymphoblastic leukemia cell line and its MDR variants, CEM/VLB55–8, and CEM/VLB100 [33 (link)], HeyA8 human ovarian cancer cell line and its MDR subline HeyA8-MDR, MCF-7 human breast cancer cell line and its MDR variant MCF7-MDR (originally named MCF-7/Adr) cells [34 (link)] were kindly provided by Dr. Fiedler (MD Anderson, TX, USA). 17-AAG and AUY922 were purchased from Enzo Life Sciences Inc. (Farmingdale, New York, USA) and Selleck Chemicals (Houston, TX, USA), respectively. EX527 was purchased from BioVision Inc. (Milpitas, CA, USA). Amurensin G, a natural SIRT1 inhibitor, was supplied Dr. Oh (Seoul National University, Seoul, Korea) as described previously [35 (link)].
3
Isolation and Characterization of CD44-Expressing Cells
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Human K562 CML cell line was obtained from American Type Culture Collection (Manassas, VA). CD44high K562 cells were established during isolation of imatinib-resistant K562 cells after treatment with increasing concentrations of imatinib, and were stable in complete medium without imatinib. The profile of cell surface CD44 expression was carried out on both cells and gated using mouse anti-human CD44-FITC (BD Biosciences, San Jose, CA, USA).We also used CD44+ HCT-15 cells with high CD44 expression and CD44- HCT-15 cells with low CD44 expression isolated from human colon cancer cell line HCT-15 29 (link). Cells were maintained in RPMI medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% (v⁄v) heat-inactivated FBS (Gibco BRL, Life Technologies, Carlsbad, CA, USA), 100 U⁄ml penicillin and 100 mg⁄ml streptomycin (Sigma-Aldrich, St.Louis, MO, USA) in a 5% CO2 humidified incubator at 37°C. 17-AAG and AUY922 were purchased from Enzo Life Sciences Inc. (Farmingdale, New York, USA) and Selleck Chemicals (Houston, TX, USA), respectively. EX527 was purchased from BioVision Inc. (Milpitas, CA, USA). Amurensin G, a natural SIRT1 inhibitor, was supplied Dr. Oh (Seoul National University, Seoul, Korea) as described previously 30 (link).
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